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Literature summary extracted from

  • Tsuru, D.; Sakabe, K.; Yoshimoto, T.; Fujiwara, K.
    Pyroglutamyl peptidase from chicken liver: purification and some properties (1982), J. Pharmacobio-Dyn., 5, 859-868.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.19.3 1,10-phenanthroline weak activation Gallus gallus
3.4.19.3 DFP weak activation Gallus gallus
3.4.19.3 EDTA weak activation Gallus gallus
3.4.19.3 PMSF weak activation Gallus gallus

General Stability

EC Number General Stability Organism
3.4.19.3 2-mercaptoethanol and EDTA stabilize Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.19.3 Cd2+
-
 Gallus gallus
3.4.19.3 Hg2+
-
 Gallus gallus
3.4.19.3 iodoacetate
-
 Gallus gallus
3.4.19.3 L-trans-epoxysuccinyl-leucylamide-(4-guanido)-butane i.e. E-64, partial Gallus gallus
3.4.19.3 Ni2+
-
 Gallus gallus
3.4.19.3 PCMB
-
 Gallus gallus
3.4.19.3 peptidic calpain inhibitor i.e. EP-475, partial Gallus gallus
3.4.19.3 pyroglutamyl-D-Ala
-
 Gallus gallus
3.4.19.3 pyroglutamyl-Pro
-
 Gallus gallus
3.4.19.3 Sodium tetrathionate
-
 Gallus gallus
3.4.19.3 Zn2+
-
 Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.19.3 0.04
-
 pyroglutamate-4-methylcoumarin 7-amide
-
 Gallus gallus
3.4.19.3 0.7
-
 pyroglutamyl-2-naphthylamide
-
 Gallus gallus
3.4.19.3 0.73
-
 pyroglutamyl-p-nitroanilide
-
 Gallus gallus
3.4.19.3 0.89
-
 L-pyroglutamyl-L-Ala
-
 Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.19.3 86000
-
 gel filtration Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.3 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.19.3
-
 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.19.3 liver
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.19.3 3.45
-
-
 Gallus gallus

Storage Stability

EC Number Storage Stability Organism
3.4.19.3 -20°C, rapid inactivation in presence of EDTA and 2-mercaptoethanol Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.3 bradykinin potentiator + H2O
-
 Gallus gallus pyroglutamate + ?
-
 ?
3.4.19.3 L-pyroglutamyl-L-Ala + H2O
-
 Gallus gallus L-pyroglutamate + L-Ala
-
 ?
3.4.19.3 litorin + H2O frog litorin Gallus gallus pyroglutamate + Gln-Trp-Ala-Val-Gly-His-Phe-Met-NH2
-
 ?
3.4.19.3 luteinizing-hormone-releasing hormone + H2O
-
 Gallus gallus ?
-
 ?
3.4.19.3 additional information the enzyme is inactive towards uperolein and piperidone carbonyl His-Pro-NH2 Gallus gallus ?
-
 ?
3.4.19.3 N-(4-methyl-2-oxo-2H-chromen-7-yl)-5-oxo-L-prolinamide + H2O
-
 Gallus gallus 5-oxo-L-proline + 7-amino-4-methylcoumarin
-
 ?
3.4.19.3 neurotensin + H2O
-
 Gallus gallus pyroglutamate + Leu-Tyr-Glu-Asn-Lys-Pro-Ag-Arg-Pro-Tyr-Ile-Leu
-
 ?
3.4.19.3 physalaemin + H2O
-
 Gallus gallus pyroglutamate + Ala-Asp-Pro-Asn-Lys-Phe-Tyr-Gly-Leu-Met-NH2
-
 ?
3.4.19.3 pyroglutamyl-beta-naphthylamide + H2O
-
 Gallus gallus pyroglutamate + beta-naphthylamine
-
 ?
3.4.19.3 pyroglutamyl-D-Ala + H2O slight activity Gallus gallus pyroglutamate + D-Ala
-
 ?
3.4.19.3 pyroglutamyl-p-nitroanilide + H2O
-
 Gallus gallus pyroglutamate + p-nitroaniline
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.19.3 50
-
 pH 7.8, 15 min, stable Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.19.3 7 8 hydrolysis of pyroglutamyl-2-naphthylamide or pyroglutamyl-Ala Gallus gallus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.19.3 7 8 30°C, 30 min, stable in presence of 5 mM each of EDTA and 2-mercaptoethanol Gallus gallus
3.4.19.3 7 8.2 stable Gallus gallus