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Literature summary extracted from
- Purification and characterization of an N-acylaminoacyl-peptide hydrolase from rabbit muscle (1989), J. Biol. Chem., 264, 11076-11081.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.19.1 | DTNB | - |
Oryctolagus cuniculus |  |
| 3.4.19.1 | NaCl | 1 mM, 78% loss of activity | Oryctolagus cuniculus | |
| 3.4.19.1 | NEM | - |
Oryctolagus cuniculus | |
| 3.4.19.1 | PCMB | - |
Oryctolagus cuniculus | |
| 3.4.19.1 | SDS | - |
Oryctolagus cuniculus | |
| 3.4.19.1 | thimerosal | - |
Oryctolagus cuniculus | |
| 3.4.19.1 | Urea | - |
Oryctolagus cuniculus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.19.1 | cytosol | - |
Oryctolagus cuniculus | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.1 | 230000 | 245000 | gel filtration | Oryctolagus cuniculus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.19.1 | Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.19.1 | - |
Oryctolagus cuniculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.19.1 | muscle | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.1 | additional information | - |
- |
Oryctolagus cuniculus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.19.1 | -20°C, 0.05 mM sodium phosphate, pH 6.9, 2 mM MgCl2, 1 mM EDTA, stable over several months | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.19.1 | additional information | rapid removal of acetyl-Thr, acetyl-Ala, acetyl-Met, acetyl-Ser and more slowly acetyl-Gly from peptides of different lengths. Other N-acetylated amino acids, Cys, Tyr, Asp, Val, Phe, Ile, Leu, may be removed at 1% or less of the rate of the good substrates | Oryctolagus cuniculus | ? | - |
? | |
| 3.4.19.1 | N-acetyl-Met-Leu-Gly + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.19.1 | trimer | 3 * 76000-80000, SDS-PAGE | Oryctolagus cuniculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.1 | 6.9 | - |
- |
Oryctolagus cuniculus |
| 3.4.19.1 | 7 | - |
hydrolysis of N-acetyl-Met-Leu-Gly | Oryctolagus cuniculus |
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Release 2023.1 (January 2023)
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