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Literature summary extracted from

  • Chikuma, T.; Kishii, M.; Taguchi, K.; Yajima, R.; Kato, T.; Loh, Y.P.; Ishii, Y.; Tanaka, A.
    High-performance liquid chromatographic-colorimetric assay for glycine carboxypeptidase activity (1997), J. Chromatogr. B, 703, 45-51.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.17.4 additional information no effect by addition of FAD, FMN, NADPH, NADP+, NADH, NAD+ Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.4 1,10-phenanthroline 0.1 mM and 1 mM, 95.6% and 74.7% compared to a control without additives Bos taurus
3.4.17.4 Ag+ AgNO3, 0.1 mM and 1 mM, complete inhibition Bos taurus
3.4.17.4 CuSO4 complete inhibition at 1 mM, 39% inhibition at 0.1 mM Bos taurus
3.4.17.4 diisopropylphosphorofluoridate 0.1 mM and 1 mM, 32.3% and 24.3% compared to a control without additives Bos taurus
3.4.17.4 EDTA 0.1 mM and 1 mM, 105% and 96.1% compared to a control without additives Bos taurus
3.4.17.4 iodoacetate 0.1 mM and 1 mM, 110.5% and 94.8% compared to a control without additives Bos taurus
3.4.17.4 p-chloromercuriphenylsulfonic acid 0.1 mM and 1 mM, complete inhibition Bos taurus
3.4.17.4 PMSF 0.1 mM and 1 mM, 78.1% and 21.4% compared to a control without additives Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.17.4 0.0211
-
 4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-Gly
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.17.4 CaCl2 0.1 mM and 1 mM, 108.9% and 105.5% compared to a control without additives Bos taurus
3.4.17.4 MgCl2 0.1 mM and 1 mM, 109.8% and 103.4% compared to a control without additives Bos taurus
3.4.17.4 Mn2+ MnCl2, 0.1 mM and 1 mM, 106.3% and 104.2% compared to a control without additives Bos taurus
3.4.17.4 Zn2+ ZnSO4, 0.1 mM and 1 mM, 97.6% and 69.6% compared to a control without additives Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.4 Bos taurus
-
-
-
3.4.17.4 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.17.4 brain
-
 Mus musculus
-
3.4.17.4 hypophysis
-
 Bos taurus
-
3.4.17.4 kidney
-
 Mus musculus
-
3.4.17.4 liver
-
 Mus musculus
-
3.4.17.4 lung
-
 Mus musculus
-
3.4.17.4 spleen
-
 Mus musculus
-
3.4.17.4 testis
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.17.4 0.000000113
-
 six-week-old animal Mus musculus
3.4.17.4 0.000000115
-
 six-week-old animal Mus musculus
3.4.17.4 0.000000116
-
 six-week-old animal Mus musculus
3.4.17.4 0.000001917
-
 six-week-old animal Mus musculus
3.4.17.4 0.00000415
-
 six-week-old animal Mus musculus
3.4.17.4 0.00000845
-
 six-week-old animal Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.4 4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-Gly + H2O
-
 Mus musculus 4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe enzyme assay is based on the detection of the product using HPLC to separate it from the substrate ?
3.4.17.4 4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-Gly + H2O
-
 Bos taurus 4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe enzyme assay is based on the detection of the product using HPLC to separate it from the substrate ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.17.4 4.8 5.4 4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-Gly as substrate, 50 mM sodium acetate buffer Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.17.4 4.2 6.5 pH 4.2: 28% of the maximal activity, pH 6.5: 43% of the maximal activity Bos taurus