Any feedback?
Literature summary extracted from
- Immunoaffininty purification and characterization of leucine aminopeptidase from human liver (1986), J. Biol. Chem., 61, 10744-10748.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.1 | 1,10-phenanthroline | - |
Homo sapiens |  |
| 3.4.11.1 | amastatin | - |
Homo sapiens | |
| 3.4.11.1 | bestatin | - |
Homo sapiens | |
| 3.4.11.1 | EDTA | reactivation by divalent cations | Homo sapiens | |
| 3.4.11.1 | iodoacetic acid | - |
Homo sapiens | |
| 3.4.11.1 | N-ethylmaleimide | - |
Homo sapiens | |
| 3.4.11.1 | p-chloromercuribenzoate | - |
Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.1 | 4.02 | - |
leucinamide | - |
Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.11.1 | cytoplasm | - |
Homo sapiens | 5737 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.1 | Mg2+ | activation | Homo sapiens | |
| 3.4.11.1 | Mn2+ | activation | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | 326000 | 360000 | non-denaturing PAGE, gel filtration | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.1 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.11.1 | - |
Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.11.1 | liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | 58 | - |
substrate L-Leu-amide | Homo sapiens |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.11.1 | -70°C, 100 mM Tris-HCl buffer, pH 8.0, 6 weeks | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.1 | amino acid amides + H2O | - |
Homo sapiens | amino acid + NH3 | - |
? | |
| 3.4.11.1 | L-Leu-p-nitroanilide + H2O | no hydrolysis | Homo sapiens | L-leucine + p-nitroaniline | - |
? | |
| 3.4.11.1 | L-leucine amide + H2O | - |
Homo sapiens | L-leucine + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.11.1 | hexamer | 3 * ab, a: 53000, b: 65000, SDS-PAGE | Homo sapiens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | 4 | - |
2 weeks | Homo sapiens |
| 3.4.11.1 | 37 | - |
1 day | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | 10.5 | - |
- |
Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | 10.4 | 10.7 | - |
Homo sapiens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | 7.5 | 8.5 | - |
Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
