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Literature summary extracted from

  • Tsai, S.C.; Gaylor, J.L.
    Testicular sterols. V. Preparation and partial purification of a microsomal prenol pyrophosphate pyrophophohydrolase (1966), J. Biol. Chem., 241, 4043-4050.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.7.1 ATP 0.93 mM stimulates rate of hydrolysis Rattus norvegicus
3.1.7.6 ATP 0.93 mM, stimulates Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.7.1 1-butanol
-
 Rattus norvegicus
3.1.7.1 3',5'-cAMP
-
 Rattus norvegicus
3.1.7.1 arsenite
-
 Rattus norvegicus
3.1.7.1 Co2+
-
 Rattus norvegicus
3.1.7.1 Cu2+
-
 Rattus norvegicus
3.1.7.1 dimethylallyl diphosphate
-
 Rattus norvegicus
3.1.7.1 farnesoic acid
-
 Rattus norvegicus
3.1.7.1 isopentenyl diphosphate
-
 Rattus norvegicus
3.1.7.1 Mg2+ loss of about 88% of activity at 10 mM Rattus norvegicus
3.1.7.1 Mn2+
-
 Rattus norvegicus
3.1.7.1 additional information
-
 Rattus norvegicus
3.1.7.1 NaF
-
 Rattus norvegicus
3.1.7.1 Plasma albumin
-
 Rattus norvegicus
3.1.7.1 Zn2+
-
 Rattus norvegicus
3.1.7.6 (2E,6E)-3,7,11-trimethyldodeca-2,6,10-trienoic acid
-
 Rattus norvegicus
3.1.7.6 3',5'-cAMP
-
 Rattus norvegicus
3.1.7.6 arsenite 1 mM Rattus norvegicus
3.1.7.6 isopentenyl diphosphate competitive Rattus norvegicus
3.1.7.6 additional information no inhibition by 1 mM p-mercuribenzoate Rattus norvegicus
3.1.7.6 Plasma albumin
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.7.1 0.7 1.2 farnesol-diphosphate
-
 Rattus norvegicus
3.1.7.1 4 10 dimethylallyl diphosphate
-
 Rattus norvegicus
3.1.7.6 0.7 1.2 (2E,6E)-farnesyl diphosphate pH 8.8, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.7.1 microsome
-
 Rattus norvegicus
-
-
3.1.7.6 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.7.1 Mg2+ 3 mM stimulates the testicular enzyme 4-10fold Rattus norvegicus
3.1.7.6 Mg2+ slight stimulation Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.7.1 prenyl diphosphate + H2O Rattus norvegicus
-
 prenol + diphosphate
-
 ?
3.1.7.6 (2E,6E)-farnesyl diphosphate + H2O Rattus norvegicus biosynthesis of acyclic sesquiterpenoids (2E,6E)-farnesol + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.7.1 Rattus norvegicus
-
-
-
3.1.7.6 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.7.1 125fold, partially purified Rattus norvegicus
3.1.7.6 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.7.1 liver
-
 Rattus norvegicus
-
3.1.7.1 testis
-
 Rattus norvegicus
-
3.1.7.6 testis
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
3.1.7.1 frozen preperations retained activity for 1 month Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.7.1 farnesyl diphosphate + H2O
-
 Rattus norvegicus farnesol + diphosphate
-
 ?
3.1.7.1 glucose-6-phosphate + H2O
-
 Rattus norvegicus glucose + phosphate
-
 ?
3.1.7.1 isopentenyl diphosphate + H2O
-
 Rattus norvegicus isopentenol + diphosphate
-
 ?
3.1.7.1 mevalonate-5-phosphate + H2O
-
 Rattus norvegicus mevalonate + phosphate
-
 ?
3.1.7.1 additional information
-
 Rattus norvegicus ?
-
 ?
3.1.7.1 prenyl diphosphate + H2O
-
 Rattus norvegicus prenol + diphosphate
-
 ?
3.1.7.6 (2E,6E)-farnesyl diphosphate + H2O biosynthesis of acyclic sesquiterpenoids Rattus norvegicus (2E,6E)-farnesol + diphosphate
-
 ?
3.1.7.6 (2E,6E)-farnesyl diphosphate + H2O diphosphate is the major product, traces of phosphate. The amount of release of diphosphate is approximately equal to the amount of release of farnesol Rattus norvegicus (2E,6E)-farnesol + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.7.1 prenol pyrophosphate pyrophosphohydrolase
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.7.1 7.8
-
 enzyme in crude acetone powder Rattus norvegicus
3.1.7.1 8.8
-
 purified enzyme Rattus norvegicus
3.1.7.6 8.8
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.7.6 8.5 9.1 pH 8.5: about 40% of maximal activity, pH 9.1: about 65% of maximal activity Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.7.6 5.2
-
 isopentenyl diphosphate pH 8.8, 37°C Rattus norvegicus