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Literature summary extracted from

  • Ikura, Y.; Horikoshi, K.
    Manganese-dependent production, and properties, of 5'-nucleotidase by alkalophilic Bacillus No. A-59 (1989), Agric. Biol. Chem., 53, 645-649.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 B4O72-
-
Bacillus sp. (in: Bacteria)
3.1.3.5 NaAsO2
-
Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 periplasm
-
Bacillus sp. (in: Bacteria)
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.5 78000
-
gel filtration Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Bacillus sp. (in: Bacteria)
-
No. A-59
-
3.1.3.5 Bacillus sp. (in: Bacteria) No. A-59
-
No. A-59
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5
-
Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.5 57.3
-
-
Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O
-
Bacillus sp. (in: Bacteria) adenosine + phosphate
-
?
3.1.3.5 5'-AMP + H2O
-
Bacillus sp. (in: Bacteria) No. A-59 adenosine + phosphate
-
?
3.1.3.5 5'-CMP + H2O 23.1% of the activity with 5'-AMP Bacillus sp. (in: Bacteria) cytidine + phosphate
-
?
3.1.3.5 5'-dAMP + H2O i.e. 2'-deoxyadenosine 5'-phosphate Bacillus sp. (in: Bacteria) deoxyadenosine + phosphate
-
?
3.1.3.5 5'-dAMP + H2O 19.7% of the activity with 5'-AMP Bacillus sp. (in: Bacteria) deoxyadenosine + phosphate
-
?
3.1.3.5 5'-dAMP + H2O i.e. 2'-deoxyadenosine 5'-phosphate Bacillus sp. (in: Bacteria) No. A-59 deoxyadenosine + phosphate
-
?
3.1.3.5 5'-dAMP + H2O 19.7% of the activity with 5'-AMP Bacillus sp. (in: Bacteria) No. A-59 deoxyadenosine + phosphate
-
?
3.1.3.5 5'-GMP + H2O 16.7% of the activity with 5'-AMP Bacillus sp. (in: Bacteria) guanosine + phosphate
-
?
3.1.3.5 5'-GMP + H2O 16.7% of the activity with 5'-AMP Bacillus sp. (in: Bacteria) No. A-59 guanosine + phosphate
-
?
3.1.3.5 5'-TMP + H2O 7.4% of the activity with 5'-AMP Bacillus sp. (in: Bacteria) thymidine + phosphate
-
?
3.1.3.5 5'-UMP + H2O 10.6% of the activity with 5'-AMP Bacillus sp. (in: Bacteria) uridine + phosphate
-
?
3.1.3.5 5'-UMP + H2O 10.6% of the activity with 5'-AMP Bacillus sp. (in: Bacteria) No. A-59 uridine + phosphate
-
?
3.1.3.5 ADP + H2O 9.6% of the activity with 5'-AMP Bacillus sp. (in: Bacteria) adenosine + diphosphate
-
?
3.1.3.5 cyclic 3',5'-AMP + H2O 8.8% of the activity with 5'-AMP Bacillus sp. (in: Bacteria) ?
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.5 40
-
15 min, 2% loss of activity Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.5 9 9.5
-
Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.5 8.5 9.5
-
Bacillus sp. (in: Bacteria)