Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Francois, J.; Van Schaftingen, E.; Hers, H.G.
    Characterization of phosphofructokinase 2 and of enzymes involved in the degradation of fructose 2,6-bisphosphate in yeast (1988), Eur. J. Biochem., 171, 599-608.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.46 benzoate
-
 Saccharomyces cerevisiae
3.1.3.46 salicylate
-
 Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.105 citrate
-
 Saccharomyces cerevisiae
2.7.1.105 Glycerol 2-phosphate
-
 Saccharomyces cerevisiae
2.7.1.105 phosphoenolpyruvate
-
 Saccharomyces cerevisiae
3.1.3.46 fructose 6-phosphate
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.105 additional information
-
 additional information kinetic data of native and phosphorylated enzyme Saccharomyces cerevisiae
3.1.3.46 0.0001
-
 fructose 2,6-bisphosphate
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.105 Mg2+
-
 Saccharomyces cerevisiae
2.7.1.105 phosphate requirement Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.105 600000
-
 gel filtration Saccharomyces cerevisiae
3.1.3.46 56000
-
 2 * 56000 Saccharomyces cerevisiae
3.1.3.46 110000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.46 fructose 2,6-bisphosphate + H2O Saccharomyces cerevisiae
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Saccharomyces cerevisiae
-
 baker's yeast, haploid strain X2180
-
3.1.3.46 Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.105 phosphoprotein phophorylation by cAMP-dependent protein kinase causes activation Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.105
-
 Saccharomyces cerevisiae
3.1.3.46 300fold Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.46 beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.105 0.55
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
3.1.3.46 -80°C, 10% glycerol, more than 1 week Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Saccharomyces cerevisiae ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
 Saccharomyces cerevisiae fructose 6-phosphate + phosphate
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
 Saccharomyces cerevisiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.46 dimer 2 * 56000 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.105 30
-
 assay at Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.105 2
-
 phosphoenolpyruvate 30°C Saccharomyces cerevisiae
2.7.1.105 6
-
 citrate 30°C Saccharomyces cerevisiae