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Literature summary extracted from

  • Baldi, B.G.; Scott, J.J.; Everard, J.D.; Loewus, F.A.
    Localization of constitutive phytases in lily pollen and properties of the pH 8 form (1988), Plant Sci., 56, 137-147.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.26 EDTA 5 mM, inhibits pH 8 phytase completely, no effect on pH 5 phytase Lilium longiflorum
3.1.3.26 F- 10 mM NaF, pH 5 phytase is inhibited by 80%, no effect on pH 8 phytase Lilium longiflorum
3.1.3.72 EDTA 5 mM, complete inhibition due to a dependence on Ca2+ Lilium longiflorum
3.1.3.72 additional information not inhibited by flouride Lilium longiflorum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.26 membrane storage body membrane Lilium longiflorum 16020
-
3.1.3.72 membrane integral component of the storage body membrane Lilium longiflorum 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.72 Ca2+ activates Lilium longiflorum
3.1.3.72 Ca2+ dependence on Ca2+ Lilium longiflorum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.26 additional information Lilium longiflorum constitutive enzyme ?
-
 ?
3.1.3.72 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Lilium longiflorum important component of phytic acid metabolism, constitutive enzyme myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.26 Lilium longiflorum
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.26 Lilium longiflorum
-
 Thunb. Cv. Nellie White, 2 enzyme forms pH 5 phytase and pH 8 phytase
-
3.1.3.72 Lilium longiflorum
-
 cv. Nellie White
-
3.1.3.72 Pinus ponderosa
-
-
-
3.1.3.72 Typha latifolia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.72 recovery is most complete when detergent or phospholipase C is included in extracting medium Lilium longiflorum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.26 pollen
-
 Lilium longiflorum
-
3.1.3.72 pollen
-
 Pinus ponderosa
-
3.1.3.72 pollen
-
 Typha latifolia
-
3.1.3.72 pollen localized in mature pollen, germination results in a rapid increase of enzyme that persists at the higher level for at least 6 h Lilium longiflorum
-
3.1.3.72 pollen at the inner surfaces of storage bodies, the phytin-containing organelles in pollen, integral component of the membrane Lilium longiflorum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.26 glucose 1-phosphate + H2O
-
 Lilium longiflorum glucose + phosphate
-
 ?
3.1.3.26 additional information constitutive enzyme Lilium longiflorum ?
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Lilium longiflorum myo-inositol pentakisphosphate + phosphate
-
 ?
3.1.3.26 myo-inositol-1,2,3,4,5 pentakisphosphate + H2O 12% of the activity with myo-inositol 1,2,3,4,5,6-hexakisphosphate Lilium longiflorum ?
-
 ?
3.1.3.26 myo-inositol-1,2,3,4,6 pentakisphosphate + H2O 22% of the activity with myo-inositol 1,2,3,4,5,6-hexakisphosphate Lilium longiflorum ?
-
 ?
3.1.3.26 myo-inositol-1,2,4,5,6 pentakisphosphate + H2O 16% of the activity with myo-inositol 1,2,3,4,5,6-hexakisphosphate Lilium longiflorum ?
-
 ?
3.1.3.26 myo-inositol-1,3,4,5,6 pentakisphosphate + H2O 27% of the activity with myo-inositol 1,2,3,4,5,6-hexakisphosphate Lilium longiflorum ?
-
 ?
3.1.3.72 additional information myo-inositol pentakisphosphates are no good substrates, terminal hydrolysis to myo-inositol trisphosphate Lilium longiflorum ?
-
 ?
3.1.3.72 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O high substrate specificity for phytic acid Lilium longiflorum myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate
-
 ?
3.1.3.72 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O important component of phytic acid metabolism, constitutive enzyme Lilium longiflorum myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.72 pH 8 phytase
-
 Lilium longiflorum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.72 37
-
 assay at Lilium longiflorum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.26 55
-
 half-life: 80 min Lilium longiflorum
3.1.3.72 additional information
-
 heat stable Lilium longiflorum
3.1.3.72 55
-
 40 min: no loss of activity, 80 min: 50% loss of activity Lilium longiflorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.26 8
-
-
 Lilium longiflorum
3.1.3.72 8
-
-
 Lilium longiflorum