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Literature summary extracted from

  • Mahajan, A.; Dua, S.
    Nonchemical approach for reducing antinutritional factors in rapeseed (Brassica campestris var. Toria) and characterization of enzyme phytase (1997), J. Agric. Food Chem., 45, 2504-2508.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.26 EDTA promotes activity Brassica rapa subsp. oleifera

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.26 arsenate
-
 Brassica rapa subsp. oleifera
3.1.3.26 Cu2+
-
 Brassica rapa subsp. oleifera
3.1.3.26 F-
-
 Brassica rapa subsp. oleifera
3.1.3.26 Fe2+
-
 Brassica rapa subsp. oleifera
3.1.3.26 molybdate
-
 Brassica rapa subsp. oleifera
3.1.3.26 phosphate 4.0 mM, 75.5% of phytase 1 activity, 80.2% inhibition of phytase 2 activity Brassica rapa subsp. oleifera
3.1.3.26 Zn2+
-
 Brassica rapa subsp. oleifera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.26 0.27
-
 myo-inositol hexakisphosphate phytase 2 Brassica rapa subsp. oleifera
3.1.3.26 0.37
-
 myo-inositol hexakisphosphate phytase 1 Brassica rapa subsp. oleifera

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.26 Co2+ activates Brassica rapa subsp. oleifera
3.1.3.26 Mn2+ activates Brassica rapa subsp. oleifera

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.26 Brassica rapa subsp. oleifera
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.26 Brassica rapa subsp. oleifera
-
 var. toria
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.26 2 phytase fractions: phytase 1 and phytase 2 Brassica rapa subsp. oleifera

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.26 additional information
-
-
 Brassica rapa subsp. oleifera

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Brassica rapa subsp. oleifera myo-inositol pentakisphosphate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.26 50
-
-
 Brassica rapa subsp. oleifera

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.26 50
-
 stable up to Brassica rapa subsp. oleifera
3.1.3.26 70
-
 complete inactivation after 50 min Brassica rapa subsp. oleifera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.26 5.2
-
 phytase 1 and 2 Brassica rapa subsp. oleifera