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Literature summary extracted from

  • Lolas, G.M.; Markakis, P.
    The phytase of navy beans (Phaseolus aureus) (1977), J. Food Sci., 42, 1094-1097.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.1.3.26 the enzyme can withstand repeated freezing and thawing without appreciable loss of acticity Phaseolus vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.26 F- 10 mM, 80% loss of activity Phaseolus vulgaris
3.1.3.26 iodoacetamide no inhibition up to 10 mM Phaseolus vulgaris
3.1.3.26 myo-inositol hexakisphosphate above 1-2 mM Phaseolus vulgaris
3.1.3.26 NaCl 0.1 M, 10% loss of activity, 0.5 M, 50% loss of activity Phaseolus vulgaris
3.1.3.26 NEM no inhibition up to 10 mM Phaseolus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.26 0.018
-
 myo-inositol hexakisphosphate
-
 Phaseolus vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.26 Co2+ 1 mM, 35% increase in activity Phaseolus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.26 Phaseolus vulgaris
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.26
-
 Phaseolus vulgaris

Storage Stability

EC Number Storage Stability Organism
3.1.3.26 2°C, 0.01 M Tris-maleate buffer, pH 6.5, stable for at least 6 weeks Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.26 AMP + H2O 9.7fold higher activity than with myo-inositol hexakisphosphate Phaseolus vulgaris adenosine + phosphate
-
 ?
3.1.3.26 diphosphate + H2O 735fold higher activity than with myo-inositol hexakisphosphate Phaseolus vulgaris 2 phosphate
-
 ?
3.1.3.26 glycerol 2-phosphate + H2O 19fold higher activity than with myo-inositol hexakisphosphate Phaseolus vulgaris glycerol + phosphate
-
 ?
3.1.3.26 glycerol 3-phosphate + H2O 15fold higher activity than with myo-inositol hexakisphosphate Phaseolus vulgaris glycerol + phosphate
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Phaseolus vulgaris myo-inositol pentakisphosphate + phosphate
-
 ?
3.1.3.26 phenyl phosphate + H2O 61.7fold higher activity than with myo-inositol hexakisphosphate Phaseolus vulgaris phenol + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.26 50
-
-
 Phaseolus vulgaris

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.26 additional information
-
-
 Phaseolus vulgaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.26 50
-
 10 min, stable Phaseolus vulgaris
3.1.3.26 65
-
 10 min, 55% loss of activity Phaseolus vulgaris
3.1.3.26 80
-
 10 min, complete inactivation Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.26 5.3
-
-
 Phaseolus vulgaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.26 4.5 6 pH 4.5: about 45% of maximal activity, pH 6.0: about 60% of maximal activity Phaseolus vulgaris