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Literature summary extracted from

  • Scott, J.J.; Loewus, F.A.
    A calcium-activated phytase from pollen of Lilium longiflorum (1986), Plant Physiol., 82, 333-335.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.26 Ca2+ at concentrations higher than substrate concentration, pH 8 phytase Lilium longiflorum
3.1.3.26 Cu2+ pH 5 phytase Lilium longiflorum
3.1.3.26 EDTA
-
 Lilium longiflorum
3.1.3.26 Mg2+ pH 8 phytase Lilium longiflorum
3.1.3.26 Mn2+ pH 8 phytase Lilium longiflorum
3.1.3.72 Ca2+ at concentrations equimolar to the substrate: 313% activation, but inhibitory at higher concentrations Lilium longiflorum
3.1.3.72 EDTA 0.5 mM: 35% inhibition, 5 mM: 94% inhibition Lilium longiflorum
3.1.3.72 Mg2+
-
 Lilium longiflorum
3.1.3.72 Mn2+
-
 Lilium longiflorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.26 0.0072
-
 myo-inositol hexakisphosphate
-
 Lilium longiflorum
3.1.3.72 0.0072
-
 myo-inositol-1,2,3,4,5,6-hexakisphosphate in presence of Ca2+ Lilium longiflorum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.26 Ca2+ 2 mM, pH 8 phytase, more than 300% activation at concentrations equimolar to the substrate, pH 8 Lilium longiflorum
3.1.3.26 Sr2+ activates Lilium longiflorum
3.1.3.26 Sr2+ 2 mM, pH 8 phytase, about 250% activation at pH 8 Lilium longiflorum
3.1.3.72 Ca2+ at concentrations equimolar to the substrate: 313% activation, but inhibitory at higher concentrations Lilium longiflorum
3.1.3.72 additional information not activated by Mg2+, Mn2+, Cu2+ and Co2+ Lilium longiflorum
3.1.3.72 Sr2+ activates Lilium longiflorum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.26 88000
-
 pH 8 phytase, gel filtration Lilium longiflorum
3.1.3.72 88000
-
 gel filtration Lilium longiflorum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.72 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Lilium longiflorum enzyme activity may be regulated by the intracellular Ca2+ concentration myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.26 Lilium longiflorum
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.26 Lilium longiflorum
-
 Thunb. Cv. Nellie White, 2 enzyme forms pH 5 phytase and pH 8 phytase
-
3.1.3.72 Lilium longiflorum
-
 cv. Nellie White
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.26 partial Lilium longiflorum
3.1.3.72 partial purification, extraction with 0.1% Triton X-100 increases recovery by nearly 4fold Lilium longiflorum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.26 pollen
-
 Lilium longiflorum
-
3.1.3.72 pollen
-
 Lilium longiflorum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.26 0.066
-
-
 Lilium longiflorum
3.1.3.72 0.066
-
-
 Lilium longiflorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Lilium longiflorum myo-inositol pentakisphosphate + phosphate
-
 ?
3.1.3.72 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
 Lilium longiflorum myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate
-
 ?
3.1.3.72 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O enzyme activity may be regulated by the intracellular Ca2+ concentration Lilium longiflorum myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.26 55 60
-
 Lilium longiflorum
3.1.3.72 55 60
-
 Lilium longiflorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.26 8
-
-
 Lilium longiflorum
3.1.3.72 8
-
 most active in Tris-maleate, rather than in Bicine buffer Lilium longiflorum