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Literature summary extracted from

  • Sutardy; Buckle, K.A.
    The characteristics of soybean phytase (1986), J. Food Biochem., 10, 197-216.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.1.3.26 the enzyme can withstand repeated freezing and thawing without appreciable loss of acticity Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.26 2-mercaptoethanol
-
 Glycine max
3.1.3.26 Al3+ 1 mM, slight inhibition Glycine max
3.1.3.26 Ca2+ 1 mM, slight inhibition Glycine max
3.1.3.26 citrate
-
 Glycine max
3.1.3.26 Co2+ 1 mM, slight inhibition Glycine max
3.1.3.26 Cu2+ 1 mM, 23% inhibition Glycine max
3.1.3.26 EDTA
-
 Glycine max
3.1.3.26 F- NaF Glycine max
3.1.3.26 glutathione
-
 Glycine max
3.1.3.26 Hg2+ 1 mM, 22% inhibition Glycine max
3.1.3.26 iodoacetamide
-
 Glycine max
3.1.3.26 L-ascorbic acid
-
 Glycine max
3.1.3.26 L-Cys
-
 Glycine max
3.1.3.26 Mg2+ 1 mM, slight inhibition Glycine max
3.1.3.26 Mn2+ 1 mM, slight inhibition Glycine max
3.1.3.26 myo-inositol hexakisphosphate at high concentrations Glycine max
3.1.3.26 NEM
-
 Glycine max
3.1.3.26 Ni2+ 1 mM, slight inhibition Glycine max
3.1.3.26 oxalate
-
 Glycine max
3.1.3.26 Tartrate
-
 Glycine max
3.1.3.26 Zn2+ 1 mM, 25% inhibition Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.26 2.4
-
 myo-inositol hexakisphosphate
-
 Glycine max

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.26 Glycine max
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.26 partial Glycine max

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.26 0.53
-
-
 Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Glycine max myo-inositol pentakisphosphate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.26 60
-
-
 Glycine max

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.26 40 70 about 50% of maximal activity at 40°C and at 70°C Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.26 50
-
 10 min, stable up to Glycine max
3.1.3.26 60
-
 10 min, 5% loss of activity Glycine max
3.1.3.26 70
-
 10 min, about 50% loss of activity Glycine max
3.1.3.26 90
-
 10 min, complete inactivation Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.26 4.8
-
-
 Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.26 4.2 5.3 pH 4.2: about 70% of maximal activity, pH 5.3: about 50% of maximal activity Glycine max