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Literature summary extracted from

  • Yamada, H.; Okamoto, K.; Kodama, K.; Noguchi, F.; Tanaka, S.
    Enzymatic studies on mannitol formation by Piricularia oryzae (1961), J. Biochem., 49, 404-410.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.3.22 rather unstable in presence of ammonium sulfate Pyricularia oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.22 ammonium sulfate complete inactivation with higher concentrations Pyricularia oryzae
3.1.3.22 CaCl2
-
Pyricularia oryzae
3.1.3.22 CuSO4
-
Pyricularia oryzae
3.1.3.22 EDTA complete inhibition at 5 mM Pyricularia oryzae
3.1.3.22 NaCN slightly inhibitory Pyricularia oryzae
3.1.3.22 NaF slightly inhibitory Pyricularia oryzae
3.1.3.22 NiCl2
-
Pyricularia oryzae
3.1.3.22 PCMB partially restored by addition of cysteine Pyricularia oryzae
3.1.3.22 ZnSO4
-
Pyricularia oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.22 3
-
mannitol 1-phosphate
-
Pyricularia oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.22 Fe2+ 2.5 fold activation Pyricularia oryzae
3.1.3.22 Mg2+
-
Pyricularia oryzae
3.1.3.22 Mn2+ 2 fold activation Pyricularia oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.22 D-mannitol 1-phosphate + H2O Neurospora crassa
-
D-mannitol + phosphate
-
?
3.1.3.22 D-mannitol 1-phosphate + H2O Aspergillus niger
-
D-mannitol + phosphate
-
?
3.1.3.22 D-mannitol 1-phosphate + H2O Pyricularia oryzae
-
D-mannitol + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.22 Aspergillus niger
-
-
-
3.1.3.22 Aspergillus oryzae
-
-
-
3.1.3.22 Neurospora crassa
-
-
-
3.1.3.22 Neurospora sitophila
-
-
-
3.1.3.22 Pyricularia oryzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.22 MnCl2 fractionation, ammonium sulfate precipitation Pyricularia oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.22 D-mannitol 1-phosphate + H2O
-
Neurospora crassa D-mannitol + phosphate
-
?
3.1.3.22 D-mannitol 1-phosphate + H2O
-
Aspergillus niger D-mannitol + phosphate
-
?
3.1.3.22 D-mannitol 1-phosphate + H2O
-
Pyricularia oryzae D-mannitol + phosphate
-
?
3.1.3.22 mannitol 1-phosphate + H2O
-
Neurospora crassa mannitol + phosphate
-
?
3.1.3.22 mannitol 1-phosphate + H2O
-
Aspergillus niger mannitol + phosphate
-
?
3.1.3.22 mannitol 1-phosphate + H2O
-
Aspergillus oryzae mannitol + phosphate
-
?
3.1.3.22 mannitol 1-phosphate + H2O
-
Neurospora sitophila mannitol + phosphate
-
?
3.1.3.22 mannitol 1-phosphate + H2O other phosphate compounds than mannitol 1-phosphate not hydrolysed Pyricularia oryzae mannitol + phosphate
-
ir

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.22 7
-
-
Pyricularia oryzae