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Literature summary extracted from

  • Oishi, H.; Tsuda, S.; Watanabe, Y.; Tamai, Y.
    Purification and some properties of phospholipase B from Schizosaccharomyces pombe (1996), Biosci. Biotechnol. Biochem., 60, 1087-1092.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.5 sodium deoxycholate 0.1%, stimulates Schizosaccharomyces pombe

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.5 cetyltrimethylammonium bromide
-
 Schizosaccharomyces pombe
3.1.1.5 EDTA
-
 Schizosaccharomyces pombe
3.1.1.5 Fe2+
-
 Schizosaccharomyces pombe
3.1.1.5 Fe3+
-
 Schizosaccharomyces pombe
3.1.1.5 SDS
-
 Schizosaccharomyces pombe

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.5 Ca2+ CaCl2, 10 mM, stimulates Schizosaccharomyces pombe
3.1.1.5 CoCl2 10 mM, stimulates Schizosaccharomyces pombe
3.1.1.5 CuSO4 10 mM, stimulates Schizosaccharomyces pombe
3.1.1.5 HgCl2 10 mM, stimulates Schizosaccharomyces pombe
3.1.1.5 MgCl2 10 mM, stimulates Schizosaccharomyces pombe
3.1.1.5 MnCl2 10 mM, stimulates Schizosaccharomyces pombe
3.1.1.5 NiSO4 10 mM, stimulates Schizosaccharomyces pombe
3.1.1.5 ZnCl2 10 mM, stimulates Schizosaccharomyces pombe

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.5 300000
-
 gel filtration Schizosaccharomyces pombe

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.5 Schizosaccharomyces pombe
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.5 side-chain modification glycoprotein Schizosaccharomyces pombe

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.5
-
 Schizosaccharomyces pombe

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.5 2.71
-
-
 Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.5 lysophosphatidylcholine + H2O 249% of the activity with phosphatidylcholine Schizosaccharomyces pombe glycerophosphorylcholine + unesterified fatty acid
-
 ?
3.1.1.5 lysophosphatidylethanolamine + H2O 27% of the activity with phosphatidylcholine Schizosaccharomyces pombe fatty acid + glycerylphosphoethanolamine
-
 ?
3.1.1.5 phosphatidic acid + H2O 62% of the activity with phosphatidylcholine Schizosaccharomyces pombe ?
-
 ?
3.1.1.5 phosphatidylcholine + H2O
-
 Schizosaccharomyces pombe lysophosphatidylcholine + glycerophosphorylcholine + a carboxylate
-
 ?
3.1.1.5 phosphatidylinositol + H2O 819% of the activity with phosphatidylcholine Schizosaccharomyces pombe lysophosphatidylinositol + glycerophosphorylinositol + a carboxylate
-
 ?
3.1.1.5 phosphatidylserine + H2O 22% of the activity with phosphatidylcholine Schizosaccharomyces pombe lysophosphatidylserine + glycerophosphorylserine + a carboxylate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.5 dimer 2 * or 3 x * 100000-150000, SDS-PAGE Schizosaccharomyces pombe
3.1.1.5 trimer 2 * or 3 x * 100000-150000, SDS-PAGE Schizosaccharomyces pombe

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.5 35
-
-
 Schizosaccharomyces pombe

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.5 55
-
 30 min, 95% loss of activity Schizosaccharomyces pombe

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.5 2.5
-
-
 Schizosaccharomyces pombe

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.5 2.2 3 pH 2.2: about 70% of maximal activity, pH 3.0: about 65% of maximal activity Schizosaccharomyces pombe

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.5 2.2 6.5 stable between pH 2.2 and pH 6.5 Schizosaccharomyces pombe