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Literature summary extracted from

  • Frantz, B.; Ngai, K.L.; Chatterjee, D.K.; Ornston, L.N.; Chakrabarty, A.M.
    Nucleotide sequence and expression of clcD, a plasmid-borne dienelactone hydrolase gene from Pseudomonas sp. strain B13 (1987), J. Bacteriol., 169, 704-709.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.45 AC866 expression in Escherichia coli and Pseudomonas putida Pseudomonas putida
3.1.1.45 expression in Escherichia coli and Pseudomonas putida Pseudomonas knackmussii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.45 25490
-
 calculated from nucleotide sequence of the clcD gene product Pseudomonas putida
3.1.1.45 25490
-
 calculated from nucleotide sequence of the clcD gene product Pseudomonas knackmussii
3.1.1.45 28000
-
 AC866, 1 * 28000, SDS-PAGE Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.45 4-carboxymethylenebut-2-en-4-olide + H2O Pseudomonas knackmussii the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.45 Pseudomonas knackmussii
-
 dienelactone hydrolases purified from Pseudomonas sp. B13 and from a clone with pAC27, encoding the 3-chlorobenzoate degradative enzymes in Pseudomonas putida AC866 are identical in every discernible respect. The DLH of Pseudomonas sp. B13 and Pseudomonas putida AC866, pAC27, are therefore not differentiated
-
3.1.1.45 Pseudomonas putida
-
 dienelactone hydrolases purified from Pseudomonas sp. B13 and from a clone with pAC27, encoding the 3-chlorobenzoate degradative enzymes in Pseudomonas putida AC866 are identical in every discernible respect. The DLH of Pseudomonas sp. B13 and Pseudomonas putida AC866, pAC27, are therefore not differentiated
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.45
-
 Pseudomonas putida
3.1.1.45
-
 Pseudomonas knackmussii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.45 4-carboxymethylenebut-2-en-4-olide + H2O the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds Pseudomonas knackmussii ?
-
 ?
3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas putida 4-oxohex-2-enedioate
-
 ?
3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas knackmussii 4-oxohex-2-enedioate
-
 ?
3.1.1.45 additional information muconolactone is not hydrolyzed to a significant extent Pseudomonas knackmussii ?
-
 ?
3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas putida 4-oxohex-2-enedioate
-
 ?
3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas knackmussii 4-oxohex-2-enedioate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.45 monomer 1* 28000, SDS-PAGE with mercaptoethanol Pseudomonas knackmussii
3.1.1.45 monomer AC866, 1 * 28000, SDS-PAGE Pseudomonas putida