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Literature summary extracted from

  • Schlömann, M.; Schmidt, E.; Knackmuss, H.J.
    Different types of dienelactone hydrolase in 4-fluorobenzoate-utilizing bacteria (1990), J. Bacteriol., 172, 5112-5118.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.45 Alcaligenes sp.
-
 type I DLH: trans-dienelactone hydrolyse
-
3.1.1.45 Alcaligenes sp. A7
-
 type I DLH: trans-dienelactone hydrolyse
-
3.1.1.45 Burkholderia cepacia
-
 type II DLH: cis-dienelactone hydrolase
-
3.1.1.45 Burkholderia cepacia
-
 Pseudomonas cepacia
-
3.1.1.45 Cupriavidus necator
-
 Alcaligenes eutrophus JMP134, type III DLH: hydrolyzes both dienelactone isomers
-
3.1.1.45 Cupriavidus necator
-
 Alcaligenes eutrophus H16, type I DLH: trans-dienelactone hydrolyse
-
3.1.1.45 Cupriavidus necator
-
 Alcaligenes eutrophus 335, type I DLH: trans-dienelactone hydrolyse
-
3.1.1.45 Cupriavidus necator
-
 Alcaligenes eutrophus JMP222, type I DLH: trans-dienelactone hydrolyse
-
3.1.1.45 Pseudomonas knackmussii
-
 type III DLH: hydrolyzes both dienelactone isomers
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.45 additional information
-
-
 Cupriavidus necator
3.1.1.45 additional information
-
-
 Alcaligenes sp.
3.1.1.45 additional information
-
-
 Burkholderia cepacia
3.1.1.45 0.01
-
 after growth with benzoate, substrate trans-4-carboxymethylenebut-2-en-4-olide Alcaligenes sp.
3.1.1.45 0.021
-
 Alcaligenes eutrophus 335 after growth with 4-hydroxy-benzoate, substrate trans-4-carboxymethylenebut-2-en-4-olide Cupriavidus necator
3.1.1.45 0.025
-
 Alcaligenes eutrophus 335 after growth with benzoate, substrate trans-4-carboxymethylenebut-2-en-4-olide Cupriavidus necator
3.1.1.45 0.031
-
 Alcaligenes eutrophus 335 after growth with succinate, substrate trans-4-carboxymethylenebut-2-en-4-olide Cupriavidus necator
3.1.1.45 0.047
-
 after growth with succinate, substrate trans-4-carboxymethylenebut-2-en-4-olide Alcaligenes sp.
3.1.1.45 0.055
-
 after growth with 4-fluorobenzoate, substrate trans-4-carboxymethylenebut-2-en-4-olide Alcaligenes sp.
3.1.1.45 0.065
-
 after growth with 4-fluorobenzoate, substrate cis-4-carboxymethylenebut-2-en-4-olide Burkholderia cepacia
3.1.1.45 0.18
-
 Alcaligenes eutrophus JMP222 after growth with 4-fluorobenzoate, substrate trans-4-carboxymethylenebut-2-en-4-olide Cupriavidus necator
3.1.1.45 0.29
-
 Alcaligenes eutrophus JMP134 after growth with 4-fluorobenzoate, substrate cis-4-carboxymethylenebut-2-en-4-olide Cupriavidus necator
3.1.1.45 0.53
-
 Alcaligenes eutrophus JMP134 after growth with 4-fluorobenzoate, substrate trans-4-carboxymethylenebut-2-en-4-olide Cupriavidus necator
3.1.1.45 1.4
-
 Alcaligenes eutrophus JMP134 after growth with 2,4-dichlorophenoxy-acetate, substrate cis-4-carboxymethylenebut-2-en-4-olide Cupriavidus necator
3.1.1.45 2
-
 Alcaligenes eutrophus JMP134 after growth with 2,4-dichlorophenoxy-acetate, substrate trans-4-carboxymethylenebut-2-en-4-olide Cupriavidus necator
3.1.1.45 4.6
-
 Alcaligenes eutrophus 335 after growth with 4-fluorobenzoate, substrate trans-4-carboxymethylenebut-2-en-4-olide Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Burkholderia cepacia 4-oxohex-2-enedioate
-
 ?
3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas knackmussii 4-oxohex-2-enedioate
-
 ?
3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O Alcaligenes eutrophus JPM134 Cupriavidus necator 4-oxohex-2-enedioate
-
 ?
3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O Alcaligenes eutrophus 335, Alcaligenes eutrophus H16, Alcaligenes eutrophus JMP222: not a substrate to a significant extent Cupriavidus necator 4-oxohex-2-enedioate
-
 ?
3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O not a substrate to a significant extent Alcaligenes sp. 4-oxohex-2-enedioate
-
 ?
3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O not a substrate to a significant extent Alcaligenes sp. A7 4-oxohex-2-enedioate
-
 ?
3.1.1.45 additional information substrate specificities differentiate among three types of this activity. Type I: extracts of Alcaligenes eutrophus 335, Alcaligenes eutrophus H16, Alcaligenes eutrophus JMP222 and Alcaligenes strain A7 convert trans-4-carboxymethylenebut-2-en-4-olide, trans-dienelactone, much faster than cis-isomer, type II: enzyme of Pseudomonas cepacia converts cis-dienelactone much faster, type III: enzyme of Alcaligenes eutrophus JMP134 and Pseudomonas strain B13 hydrolyze both dienelactones Cupriavidus necator ?
-
 ?
3.1.1.45 additional information substrate specificities differentiate among three types of this activity. Type I: extracts of Alcaligenes eutrophus 335, Alcaligenes eutrophus H16, Alcaligenes eutrophus JMP222 and Alcaligenes strain A7 convert trans-4-carboxymethylenebut-2-en-4-olide, trans-dienelactone, much faster than cis-isomer, type II: enzyme of Pseudomonas cepacia converts cis-dienelactone much faster, type III: enzyme of Alcaligenes eutrophus JMP134 and Pseudomonas strain B13 hydrolyze both dienelactones Alcaligenes sp. ?
-
 ?
3.1.1.45 additional information substrate specificities differentiate among three types of this activity. Type I: extracts of Alcaligenes eutrophus 335, Alcaligenes eutrophus H16, Alcaligenes eutrophus JMP222 and Alcaligenes strain A7 convert trans-4-carboxymethylenebut-2-en-4-olide, trans-dienelactone, much faster than cis-isomer, type II: enzyme of Pseudomonas cepacia converts cis-dienelactone much faster, type III: enzyme of Alcaligenes eutrophus JMP134 and Pseudomonas strain B13 hydrolyze both dienelactones Burkholderia cepacia ?
-
 ?
3.1.1.45 additional information substrate specificities differentiate among three types of this activity. Type I: extracts of Alcaligenes eutrophus 335, Alcaligenes eutrophus H16, Alcaligenes eutrophus JMP222 and Alcaligenes strain A7 convert trans-4-carboxymethylenebut-2-en-4-olide, trans-dienelactone, much faster than cis-isomer, type II: enzyme of Pseudomonas cepacia converts cis-dienelactone much faster, type III: enzyme of Alcaligenes eutrophus JMP134 and Pseudomonas strain B13 hydrolyze both dienelactones Pseudomonas knackmussii ?
-
 ?
3.1.1.45 additional information substrate specificities differentiate among three types of this activity. Type I: extracts of Alcaligenes eutrophus 335, Alcaligenes eutrophus H16, Alcaligenes eutrophus JMP222 and Alcaligenes strain A7 convert trans-4-carboxymethylenebut-2-en-4-olide, trans-dienelactone, much faster than cis-isomer, type II: enzyme of Pseudomonas cepacia converts cis-dienelactone much faster, type III: enzyme of Alcaligenes eutrophus JMP134 and Pseudomonas strain B13 hydrolyze both dienelactones Alcaligenes sp. A7 ?
-
 ?
3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Cupriavidus necator 4-oxohex-2-enedioate
-
 ?
3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Alcaligenes sp. 4-oxohex-2-enedioate
-
 ?
3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas knackmussii 4-oxohex-2-enedioate
-
 ?
3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Alcaligenes sp. A7 4-oxohex-2-enedioate
-
 ?