EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.45 | protoanemonin | competitive inhibitor of the transformation of cis-dienelactone | Pseudomonas knackmussii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.45 | 0.381 | - |
cis-4-carboxymethylenebut-2-en-4-olide | - |
Pseudomonas knackmussii | |
3.1.1.45 | 0.415 | - |
protoanemonin | - |
Pseudomonas knackmussii | |
3.1.1.45 | 1.8 | - |
trans-4-Carboxymethylenebut-2-en-4-olide | - |
Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.45 | additional information | Burkholderia cepacia | - |
? | - |
? | |
3.1.1.45 | additional information | Pseudomonas knackmussii | it seems likely, that dienelactone hydrolase has the additional function of detoxification of minor amounts of protoanemonin that may be formed during chloroaromatic degradation | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.45 | Burkholderia cepacia | - |
Pseudomonas cepacia | - |
3.1.1.45 | Pseudomonas knackmussii | - |
- |
- |
3.1.1.45 | Pseudomonas putida | - |
RW10, trans-dienelactone hydrolase | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.45 | 3-chlorobenzoate-grown cells | Pseudomonas knackmussii |
3.1.1.45 | partial | Burkholderia cepacia |
3.1.1.45 | RW10 partial | Pseudomonas putida |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.1.1.45 | 4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate | the active-site Cys123 residue is part of a triad of residues consisting of Cys123, His202 and Asp171, and is reminiscent of the serine/cysteine proteases | Pseudomonas knackmussii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.45 | 0.58 | - |
substrate protoanemonin | Pseudomonas knackmussii |
3.1.1.45 | 58.8 | - |
substrate cis-4-carboxymethylenebut-2-en-4-olide | Pseudomonas knackmussii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.45 | cis-4-carboxymethylenebut-2-en-4-olide + H2O | - |
Burkholderia cepacia | 4-oxohex-2-enedioate | - |
? | |
3.1.1.45 | cis-4-carboxymethylenebut-2-en-4-olide + H2O | - |
Pseudomonas knackmussii | 4-oxohex-2-enedioate | - |
? | |
3.1.1.45 | cis-4-carboxymethylenebut-2-en-4-olide + H2O | Pseudomonas putida RW10 not a substrate to a significant extent | Pseudomonas putida | 4-oxohex-2-enedioate | - |
? | |
3.1.1.45 | additional information | - |
Burkholderia cepacia | ? | - |
? | |
3.1.1.45 | additional information | it seems likely, that dienelactone hydrolase has the additional function of detoxification of minor amounts of protoanemonin that may be formed during chloroaromatic degradation | Pseudomonas knackmussii | ? | - |
? | |
3.1.1.45 | protoanemonin + H2O | - |
Pseudomonas knackmussii | cis-acetylacrylate | - |
? | |
3.1.1.45 | protoanemonin + H2O | activity of cis-dienelactone hydrolase with 50 microM protoanemonin is 0.08% of that observed with 50 microM cis-dienelactone, cis-4-carboxymethylenebut-2-en-4-olide | Burkholderia cepacia | cis-acetylacrylate | - |
? | |
3.1.1.45 | trans-4-carboxymethylenebut-2-en-4-olide + H2O | - |
Pseudomonas putida | 4-oxohex-2-enedioate | - |
? | |
3.1.1.45 | trans-4-carboxymethylenebut-2-en-4-olide + H2O | - |
Pseudomonas knackmussii | 4-oxohex-2-enedioate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.45 | monomer | - |
Pseudomonas putida |
3.1.1.45 | monomer | - |
Burkholderia cepacia |
3.1.1.45 | monomer | 1 * 28000-29000, SDS-PAGE | Pseudomonas knackmussii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.45 | 2.08 | - |
protoanemonin | - |
Pseudomonas knackmussii | |
3.1.1.45 | 260 | - |
cis-4-carboxymethylenebut-2-en-4-olide | - |
Pseudomonas knackmussii |