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Literature summary extracted from

  • Ollis, D.L.; Ngai, K.L.
    Crystallization and preliminary x-ray crystallographic data of dienelactone hydrolase from Pseudomonas sp. B13 (1985), J. Biol. Chem., 260, 9818-9819.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.45
-
Pseudomonas knackmussii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.45 p-chloromercuribenzoate
-
Cupriavidus necator
3.1.1.45 p-chloromercuribenzoate
-
Pseudomonas knackmussii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.45 4-carboxymethylenebut-2-en-4-olide + H2O Pseudomonas knackmussii the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.45 Burkholderia cepacia
-
Pseudomonas cepacia
-
3.1.1.45 Cupriavidus necator
-
Alcaligenes eutrophus
-
3.1.1.45 Pseudomonas knackmussii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.45 3-chlorobenzoate-grown cells Pseudomonas knackmussii

Storage Stability

EC Number Storage Stability Organism
3.1.1.45 4°C, converted to microcrystals, ammonium sulfate to 30% saturation, stable for more than 1 year Pseudomonas knackmussii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.45 4-carboxymethylenebut-2-en-4-olide + H2O the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds Pseudomonas knackmussii ?
-
?
3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O
-
Cupriavidus necator 4-oxohex-2-enedioate
-
?
3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O
-
Burkholderia cepacia 4-oxohex-2-enedioate
-
?
3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O
-
Pseudomonas knackmussii 4-oxohex-2-enedioate
-
ir
3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O
-
Cupriavidus necator 4-oxohex-2-enedioate
-
?
3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O
-
Pseudomonas knackmussii 4-oxohex-2-enedioate
-
ir

Subunits

EC Number Subunits Comment Organism
3.1.1.45 monomer
-
Burkholderia cepacia
3.1.1.45 monomer 1* 28000, SDS-PAGE with mercaptoethanol Pseudomonas knackmussii