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Literature summary extracted from

  • Kosuge, T.; Conn, E.
    The metabolism of aromatic compounds in higher plants (1962), J. Biol. Chem., 237, 1653-1656.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.35 CaCl2 no effect at a concentration of 3.3 mM Melilotus albus
3.1.1.35 CoCl2 23% inhibition at a concentration of 1.6 mM Melilotus albus
3.1.1.35 CuSO4 100% inhibition at a concentration of 3.3 mM Melilotus albus
3.1.1.35 delta-D-gluconolactone no effect at a concentration of 3.3 mM Melilotus albus
3.1.1.35 iodoacetamide no effect at a concentration of 3.3 mM Melilotus albus
3.1.1.35 MgCl2 no effect at a concentration of 3.3 mM Melilotus albus
3.1.1.35 MnCl2 no effect at a concentration of 3.3 mM Melilotus albus
3.1.1.35 NaF 20% inhibition at a concentration of 3.3 mM Melilotus albus
3.1.1.35 NiCl2 54% inhibition at a concentration of 3.3 mM Melilotus albus
3.1.1.35 ZnCl2 85% inhibition at a concentration of 3.3 mM Melilotus albus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.35 0.24
-
 dihydrocoumarin
-
 Melilotus albus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.35 3,4-dihydrocoumarin + H2O Melilotus albus coumarin is converted to dihydrocoumarin by hydrogenation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.35 Anthoxanthum occidentale
-
 Buckl.
-
3.1.1.35 Anthoxanthum occidentale Buckl.
-
 Buckl.
-
3.1.1.35 Medicago sativa
-
 L., alfalfa
-
3.1.1.35 Melilotus albus
-
 Desr., white sweet clover
-
3.1.1.35 Melilotus officinalis
-
 Lam., yellow sweet clover
-
3.1.1.35 no activity in Hordeum vulgare
-
 barley
-
3.1.1.35 Trifolium pratense
-
 L., red clover
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.35 89fold Melilotus albus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.35 root 22 micromole/min activity per g fresh weight Melilotus albus
-
3.1.1.35 seedling
-
 Melilotus albus
-
3.1.1.35 shoot 64 micromole/min activity per g fresh weight Melilotus albus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.35 1636
-
 dihydrocoumarin as substrate, after a 4-step purification Melilotus albus

Storage Stability

EC Number Storage Stability Organism
3.1.1.35 -10°C, acetone powder from seedlings, over 6 months, less than 10% loss of activity Melilotus albus
3.1.1.35 -10°C, at pH 7, preparations containing more than 5 mg per ml of protein, 12 months, less than 10% loss of activity Melilotus albus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.35 3,4-dihydrocoumarin + H2O
-
 Medicago sativa 3-(2-hydroxyphenyl)propionic acid
-
 ?
3.1.1.35 3,4-dihydrocoumarin + H2O
-
 Melilotus officinalis 3-(2-hydroxyphenyl)propionic acid
-
 ?
3.1.1.35 3,4-dihydrocoumarin + H2O
-
 Trifolium pratense 3-(2-hydroxyphenyl)propionic acid
-
 ?
3.1.1.35 3,4-dihydrocoumarin + H2O
-
 Anthoxanthum occidentale 3-(2-hydroxyphenyl)propionic acid
-
 ?
3.1.1.35 3,4-dihydrocoumarin + H2O 60% of the activity compared to 7-hydroxydihydrocoumarin Melilotus albus 3-(2-hydroxyphenyl)propionic acid
-
 ?
3.1.1.35 3,4-dihydrocoumarin + H2O
-
 Anthoxanthum occidentale Buckl. 3-(2-hydroxyphenyl)propionic acid
-
 ?
3.1.1.35 3,4-dihydrocoumarin + H2O coumarin is converted to dihydrocoumarin by hydrogenation Melilotus albus ?
-
 ?
3.1.1.35 3H-benzofuran-2-one + H2O 16% of the activity compared to 7-hydroxydihydrocoumarin Melilotus albus 2-hydroxyphenylacetic acid
-
 ?
3.1.1.35 5-hydroxy-3H-benzofuran-2-one + H2O 82% of the activity compared to 7-hydroxydihydrocoumarin Melilotus albus homogentisic acid
-
 ?
3.1.1.35 6,7-dihydroxydihydrocoumarin + H2O 6% of the activity compared to 7-hydroxydihydrocoumarin Melilotus albus 3-(2,3,6-trihydroxyphenyl)propionic acid
-
 ?
3.1.1.35 6,7-dihydroxydihydrocoumarin + H2O i.e. escelutin Melilotus albus 3-(2,3,6-trihydroxyphenyl)propionic acid
-
 ?
3.1.1.35 7,8-dihydroxydihydrocoumarin + H2O 8% of the activity compared to 7-hydroxydihydrocoumarin Melilotus albus 3-(3,4,6-trihydroxyphenyl)propionic acid
-
 ?
3.1.1.35 7-hydroxy-6-methoxydihydrocoumarin + H2O 43% of the activity compared to 7-hydroxydihydrocoumarin Melilotus albus 3-(2,3-dihydroxy-3-methoxyphenyl)propionic acid
-
 ?
3.1.1.35 7-hydroxydihydrocoumarin + H2O most rapidly hydrolyzed Melilotus albus ?
-
 ?
3.1.1.35 delta-D-gluconolactone + H2O 0.8% of the activity compared to 7-hydroxydihydrocoumarin Melilotus albus D-gluconate
-
 ?
3.1.1.35 delta-valerolactone + H2O 0.3% of the activity compared to 7-hydroxydihydrocoumarin Melilotus albus 5-hydroxypentanoate
-
 ?
3.1.1.35 additional information no hydrolysis with gamma-butyrolactone as substrate Melilotus albus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.35 55
-
 stable to heating for 10 min Melilotus albus
3.1.1.35 60
-
 after heating for 10 min, 40% loss of activity Melilotus albus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.35 1670
-
 dihydrocoumarin
-
 Melilotus albus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.35 7.8 8.2
-
 Melilotus albus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.35 7 9 pH 7: 70% of the maximal activity, pH 9: 40% of the maximal activity Melilotus albus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.35 5.8 9 no loss of activity after 2 h in buffers at 0°C Melilotus albus