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Literature summary extracted from

  • Sahsah, Y.; Thi, A.T.P.; Roy-Macauley, H.; D'Arcy-Lameta, A.; Repellin, A.; Zuily-Fodil, Y.
    Purification and characterization of a soluble lipolytic acylhydrolase from cowpea (Vigna unguiculata L.) leaves (1994), Biochim. Biophys. Acta, 1215, 66-73.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.26 0.119
-
 monogalactosyldiacylglycerol
-
 Vigna unguiculata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.26 CaCl2 increase of activity with 50-500 mM Vigna unguiculata
3.1.1.26 additional information no effect of 1 mM and 5 mM Ca2+ Vigna unguiculata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.26 40000
-
 2 * 40000, SDS-PAGE Vigna unguiculata
3.1.1.26 80000
-
 gel filtration Vigna unguiculata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.26 digalactosyldiacylglycerol + H2O Vigna unguiculata
-
 ?
-
 ?
3.1.1.26 monogalactosyldiacylglycerol + H2O Vigna unguiculata
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.26 Vigna unguiculata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.26 263fold Vigna unguiculata

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.26 0.00021
-
-
 Vigna unguiculata
3.1.1.26 0.00059
-
-
 Vigna unguiculata
3.1.1.26 0.00066
-
-
 Vigna unguiculata
3.1.1.26 0.0117
-
-
 Vigna unguiculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.26 digalactosyldiacylglycerol + H2O fatty acid with chain lengths of C16:0, C16:1, C18:1, C18:2, C18:3, the polyunsaturated substrates are preferentially hydrolyzed Vigna unguiculata digalactosylglycerol + fatty acid
-
 ?
3.1.1.26 digalactosyldiacylglycerol + H2O
-
 Vigna unguiculata ?
-
 ?
3.1.1.26 lysophosphatidylcholine + H2O
-
 Vigna unguiculata ?
-
 ?
3.1.1.26 monogalactosyldiacylglycerol + H2O substrates with chain lengths of C16:0, C16:1, C18:1, C18:2, C18:3, the polyunsaturated substrates are preferentially hydrolyzed Vigna unguiculata galactosylglycerol + fatty acid
-
 ?
3.1.1.26 monogalactosyldiacylglycerol + H2O
-
 Vigna unguiculata ?
-
 ?
3.1.1.26 phosphatidylcholine + H2O fatty acid with chain lengths of C16:0, C16:1, C18:1, C18:2, C18:3, the polyunsaturated substrates are preferentially hydrolyzed Vigna unguiculata ?
-
 ?
3.1.1.26 phosphatidylglycerol + H2O fatty acid with chain lengths of C16:0, C16:1, C18:1, C18:2, C18:3, the polyunsaturated substrates are preferentially hydrolyzed Vigna unguiculata ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.26 dimer 2 * 40000, SDS-PAGE Vigna unguiculata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.26 50
-
 maximally active after 10 min incubation Vigna unguiculata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.26 5
-
-
 Vigna unguiculata