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Literature summary extracted from

  • Barthomeuf, C.; Regerat, F.; Pourrat, H.
    Production, purification and characterization of a tannase from Aspergillus niger LCF 8 (1994), J. Ferment. Bioeng., 77, 320-323.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.20 diisopropylfluorophosphate 83% inhibition Aspergillus niger
3.1.1.20 additional information not inhibited by o-phenanthroline, PMSF, EDTA, 2-mercaptomethanol, sodium thioglycolate Aspergillus niger

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.20 Ca2+ CaCl2, no effect on the activity Aspergillus niger
3.1.1.20 Co2+ CoCl2 no effect on activity Aspergillus niger
3.1.1.20 Cu2+ CuSO4: 68% inhibition Aspergillus niger
3.1.1.20 FeCl3 13% inhibition Aspergillus niger
3.1.1.20 Mg2+ MgSO4 no effect on activity Aspergillus niger
3.1.1.20 Mn2+ MnCl2 no effect on activity Aspergillus niger
3.1.1.20 Zn2+ ZnCl2 39% inhibition Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.20 53000
-
 2 * 53000, SDS-PAGE Aspergillus niger
3.1.1.20 186000
-
 glycoprotein containing 43% sugars, nondenaturing PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.20 tannic acid + H2O Aspergillus niger
-
 ?
-
 ?
3.1.1.20 tannic acid + H2O Aspergillus niger LCF 8
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Aspergillus niger
-
 LCF 8
-
3.1.1.20 Aspergillus niger LCF 8
-
 LCF 8
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.20 side-chain modification glycoprotein, containg 43% sugars Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 29.1fold Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.20 118.8
-
 3 step purification Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 tannic acid + H2O
-
 Aspergillus niger gallic acid + ?
-
 ?
3.1.1.20 tannic acid + H2O
-
 Aspergillus niger LCF 8 gallic acid + ?
-
 ?
3.1.1.20 tannic acid + H2O
-
 Aspergillus niger ?
-
 ?
3.1.1.20 tannic acid + H2O
-
 Aspergillus niger LCF 8 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.20 dimer 2 * 53000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.20 35
-
-
 Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.20 20 45 active between these temperatures Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.20 4 45 stable between Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 6
-
 pI 4.3 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.20 3.5 8
-
 Aspergillus niger