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Literature summary extracted from

  • Abdel-Naby, M.A.; Sherif, A.A.; El-Tanash, A.B.; Mankarios, A.T.
    Immobilization of Aspergillus oryzae tannase and properties of the immobilized enzyme (1999), J. Appl. Microbiol., 87, 108-114.
No PubMed abstract available

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.20 Ca2+ CaCl2, 5 mM, no effect on the activity, both the free enzyme and the immoblized enzyme Aspergillus oryzae
3.1.1.20 Co2+ CoCl2 at 5 mM, free enzyme: 49.3% inhibition, immobilized enzyme: 24% inhibition Aspergillus oryzae
3.1.1.20 Cu2+ CuSO4 at 5 mM, free enzyme: 59% inhibition, immobilized enzyme: 44% inhibition Aspergillus oryzae
3.1.1.20 FeSO4 at 5 mM, free enzyme: 61.3% inhibition, immobilized enzyme: 28% inhibition Aspergillus oryzae
3.1.1.20 HgCl2 5 mM, free enzyme: 13.7% inhibition, immobilized enzyme: 30% inhibition Aspergillus oryzae
3.1.1.20 Mg2+ MgCl2 at 5 mM, free enzyme: 28.4% inhibition, immobilized enzyme: 14% inhibition Aspergillus oryzae
3.1.1.20 Zn2+ ZnCl2 at 5 mM, free enzyme: 55.4% inhibition, immobilized enzyme: 29.3% inhibition Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.20 tannic acid + H2O Aspergillus oryzae
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Aspergillus oryzae
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.20 14.65
-
immobilized enzyme Aspergillus oryzae
3.1.1.20 72.2
-
free enzyme Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 tannic acid + H2O
-
Aspergillus oryzae gallic acid + ?
-
?
3.1.1.20 tannic acid + H2O
-
Aspergillus oryzae 10 gallate + D-glucose
-
?
3.1.1.20 tannic acid + H2O
-
Aspergillus oryzae ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.20 40
-
free enzyme, activation energy 6.75 kcal per mol Aspergillus oryzae
3.1.1.20 55
-
immobilized enzyme, activation energy 5.77 kcal per mol Aspergillus oryzae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.20 25 75 immobilized enzyme, 25°C: 40% of the maximal activity, 75°C: 18% of the maximal activity. Free enzyme, 25°C: 57% of the maximal activity, 75°C: 10% of the maximal activity Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.20 50
-
half-life of free enzyme: 90 min, half-life of immobilized enzyme: 120 min Aspergillus oryzae
3.1.1.20 60
-
half-life of free enzyme: 18 min, half-life of immobilized enzyme: 40 min Aspergillus oryzae
3.1.1.20 70
-
half-life of free enzyme: 8 min at, half-life of immobilized enzyme: 25 min Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 4.5
-
immobilized enzyme Aspergillus oryzae
3.1.1.20 5.5
-
free enzyme Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.20 3 8 immobilized enzyme, pH 3: 40% of the maximal activity, pH 8: 20% of the maximal activity. Free enzyme, pH 3: 5% of the maximal activity, pH 8: 20% of the maximal activity Aspergillus oryzae