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Literature summary extracted from

  • Kato, Y.; Yamada, H.; Asano, Y.
    Stereoselective synthesis of opine-type secondary amine carboxylic acids by a new enzyme opine dehydrogenase. Use of recombinant enzymes (1996), J. Mol. Catal. , B Enzym., 1, 151-160.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.28 NADH
-
 Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.28 2.17
-
 (S)-norvaline pyruvate and NADH as cosubstrates Arthrobacter sp.
1.5.1.28 2.9
-
 L-Leu pyruvate and NADH as cosubstrates Arthrobacter sp.
1.5.1.28 3
-
 L-Val pyruvate and NADH as cosubstrates Arthrobacter sp.
1.5.1.28 3.19
-
 beta-chloro-(S)-alanine pyruvate and NADH as cosubstrates Arthrobacter sp.
1.5.1.28 3.72
-
 (S)-norleucine pyruvate and NADH as cosubstrates Arthrobacter sp.
1.5.1.28 5.1
-
 L-Ala pyruvate and NADH as cosubstrates Arthrobacter sp.
1.5.1.28 5.94
-
 O-acetyl-(S)-Ser pyruvate and NADH as cosubstrates Arthrobacter sp.
1.5.1.28 8.7
-
 L-Phe pyruvate and NADH as cosubstrates Arthrobacter sp.
1.5.1.28 8.7
-
 O-phospho-(S)-Ser pyruvate and NADH as cosubstrates Arthrobacter sp.
1.5.1.28 11.9
-
 (R,S)-2,3-diaminopropionic acid pyruvate and NADH as cosubstrates Arthrobacter sp.
1.5.1.28 20
-
 (S)-2-aminobutanoate pyruvate and NADH as cosubstrates Arthrobacter sp.
1.5.1.28 28.3
-
 L-phenylglycine pyruvate and NADH as cosubstrates Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.28 Arthrobacter sp.
-
-
-
1.5.1.28 Arthrobacter sp. 1C
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.28 (R,S)-2,3-Diaminopropionic acid + pyruvate + NADH 9.67% of the activity with S: (S)-norvaline Arthrobacter sp. ?
-
 ?
1.5.1.28 (R,S)-3-Aminobutanoate + pyruvate + NADH 0.68% of the activity with (S)-norvaline Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(R,S)-3-aminobutanoate + NAD+
-
 ?
1.5.1.28 (S)-2-aminobutyric acid + pyruvate + NADH 83.7% of the activity with (S)-norvaline Arthrobacter sp. N-[1-(R)-(carboxy)ethyl]-(S)-2-aminobutyric acid + NAD+
-
 ?
1.5.1.28 (S)-2-aminobutyric acid + pyruvate + NADH 83.7% of the activity with (S)-norvaline Arthrobacter sp. 1C N-[1-(R)-(carboxy)ethyl]-(S)-2-aminobutyric acid + NAD+
-
 ?
1.5.1.28 (S)-Norleucine + pyruvate + NADH 72.5% of the activity with (S)-norvaline Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-norleucine + NAD+
-
 ?
1.5.1.28 (S)-Norleucine + pyruvate + NADH 72.5% of the activity with (S)-norvaline Arthrobacter sp. 1C N-[1-(R)-(Carboxy)ethyl]-norleucine + NAD+
-
 ?
1.5.1.28 (S)-Norvaline + pyruvate + NADH
-
 Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-norvaline + NAD+
-
 ?
1.5.1.28 (S)-Norvaline + pyruvate + NADH
-
 Arthrobacter sp. 1C N-[1-(R)-(Carboxy)ethyl]-(S)-norvaline + NAD+
-
 ?
1.5.1.28 (S)-Phenylalaninol + pyruvate + NADH 0.63% of the activity with (S)-norvaline Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-phenylalaninol + NAD+
-
 ?
1.5.1.28 (S)-Phenylglycine + pyruvate + NADH 4.4% of the activity with (S)-norvaline Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-phenylglycine + NAD+
-
 ?
1.5.1.28 beta-Chloro-(S)-Ala + pyruvate + NADH 53.1% of the activity with (S)-norvaline Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-beta-chloroalanine + NAD+
-
 ?
1.5.1.28 beta-Chloro-(S)-Ala + pyruvate + NADH 53.1% of the activity with (S)-norvaline Arthrobacter sp. 1C N-[1-(R)-(Carboxy)ethyl]-(S)-beta-chloroalanine + NAD+
-
 ?
1.5.1.28 L-Ala + pyruvate + NADH 16.0% of the activity with (S)-norvaline Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Ala + NAD+
-
 ?
1.5.1.28 L-Ile + pyruvate + NADH 22.3% of the activity with (S)-norvaline Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Ile + NAD+
-
 ?
1.5.1.28 L-Leu + pyruvate + NADH 21.2% of the activity with (S)-norvaline Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Leu + NAD+
-
 ?
1.5.1.28 L-Met + pyruvate + NADH 23.9% of the activity with (S)-norvaline Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Met + NAD+
-
 ?
1.5.1.28 L-Phe + pyruvate + NADH 21.7% of the activity with (S)-norvaline Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Phe + NAD+
-
 ?
1.5.1.28 L-Val + pyruvate + NADH 21.9% of the activity with (S)-norvaline Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Val + NAD+
-
 ?
1.5.1.28 O-Acetyl-(S)-Ser + pyruvate + NADH 35.8% of the activity with (S)-norvaline Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-O-acetyl-Ser + NAD+
-
 ?
1.5.1.28 O-Acetyl-(S)-Ser + pyruvate + NADH 35.8% of the activity with (S)-norvaline Arthrobacter sp. 1C N-[1-(R)-(Carboxy)ethyl]-(S)-O-acetyl-Ser + NAD+
-
 ?
1.5.1.28 O-phospho-(S)-Ser + pyruvate + NADH + H+
-
 Arthrobacter sp. N-[1-(R)-(carboxy)ethyl]-(S)-O-phospho-Ser + NAD+
-
 r
1.5.1.28 p-Phospho-(S)-Tyr + pyruvate + NADH 0.64% of the activity with (S)-norvaline Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(R,S)-p-phospho-(S)-Tyr + NAD+
-
 ?
1.5.1.28 phospho-(S)-Ser + pyruvate + NADH 15.8% of the activity with (S)-norvaline Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-phospho-Ser + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.28 N-[1-(R)-(Carboxy)ethyl]-(S)-norvaline: NAD+ oxidoreductase (L-norvaline forming)
-
 Arthrobacter sp.
1.5.1.28 ODH
-
 Arthrobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.28 NADH
-
 Arthrobacter sp.