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Literature summary extracted from

  • Asano, Y.; Yamaguchi, K.; Kondo, K.
    A new NAD+-dependent opine dehydrogenase from Arthrobacter sp. strain 1C (1989), J. Bacteriol., 171, 4466-4471.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.28 NADH
-
 Arthrobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.28 5,5'-dithiobis(2-nitrobenzoic acid) 0.13 mM, complete inhibition Arthrobacter sp.
1.5.1.28 Ag+ 0.13 mM, complete inhibition Arthrobacter sp.
1.5.1.28 Cd2+ 0.13 mM, complete inhibition Arthrobacter sp.
1.5.1.28 Co2+
-
 Arthrobacter sp.
1.5.1.28 Cu2+ 0.13 mM, complete inhibition Arthrobacter sp.
1.5.1.28 Fe2+
-
 Arthrobacter sp.
1.5.1.28 Hg2+ 0.13 mM, complete inhibition Arthrobacter sp.
1.5.1.28 Mn2+
-
 Arthrobacter sp.
1.5.1.28 N-[1-S-(Carboxy)ethyl]-(S)-Phe
-
 Arthrobacter sp.
1.5.1.28 NEM 0.13 mM, complete inhibition Arthrobacter sp.
1.5.1.28 Ni2+ 0.13 mM, complete inhibition Arthrobacter sp.
1.5.1.28 Pb2+
-
 Arthrobacter sp.
1.5.1.28 PCMB
-
 Arthrobacter sp.
1.5.1.28 Zn2+
-
 Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.28 2.5
-
 L-Cys pyruvate and NADH as cosubstrates Arthrobacter sp.
1.5.1.28 2.9
-
 L-Leu
-
 Arthrobacter sp.
1.5.1.28 3
-
 pyruvate L-Met and NADH as cosubstrate Arthrobacter sp.
1.5.1.28 3.5
-
 oxaloacetate L-Met and NADH as cosubstrate Arthrobacter sp.
1.5.1.28 3.6
-
 L-Ser pyruvate and NADH as cosubstrates Arthrobacter sp.
1.5.1.28 4.1
-
 L-Met pyruvate and NADH as cosubstrates Arthrobacter sp.
1.5.1.28 5.1
-
 L-Ala pyruvate and NADH as cosubstrates Arthrobacter sp.
1.5.1.28 6.2
-
 L-Ile pyruvate and NADH as cosubstrates Arthrobacter sp.
1.5.1.28 7.4
-
 L-Thr pyruvate and NADH as cosubstrates Arthrobacter sp.
1.5.1.28 7.5
-
 L-Phe pyruvate and NADH as cosubstrates Arthrobacter sp.
1.5.1.28 8.8
-
 glyoxylate L-Met and NADH as cosubstrate Arthrobacter sp.
1.5.1.28 28
-
 2-Oxohexanoate L-Met and NADH as cosubstrate Arthrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.28 70000
-
 gel filtration Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.28 Arthrobacter sp.
-
-
-
1.5.1.28 Arthrobacter sp. 1C
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.28
-
 Arthrobacter sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.28 additional information
-
-
 Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.28 Gly + pyruvate + NADH 5.3% of the activity with L-Met Arthrobacter sp. N-(carboxymethyl)-D-alanine + NAD+
-
 r
1.5.1.28 L-Ala + pyruvate + NADH 67% of the activity with L-Met Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Ala + NAD+
-
 r
1.5.1.28 L-Asp + pyruvate + NADH 1.2% of the activity with L-Met Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Asp + NAD+
-
 r
1.5.1.28 L-Cys + pyruvate + NADH 39% of the activity with L-Met Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Cys + NAD+
-
 r
1.5.1.28 L-Ile + pyruvate + NADH 94% of the activity with L-Met Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Ile + NAD+
-
 r
1.5.1.28 L-Ile + pyruvate + NADH 94% of the activity with L-Met Arthrobacter sp. 1C N-[1-(R)-(Carboxy)ethyl]-(S)-Ile + NAD+
-
 r
1.5.1.28 L-Leu + pyruvate + NADH 89% of the activity with L-Met Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Leu + NAD+
-
 r
1.5.1.28 L-Leu + pyruvate + NADH 89% of the activity with L-Met Arthrobacter sp. 1C N-[1-(R)-(Carboxy)ethyl]-(S)-Leu + NAD+
-
 r
1.5.1.28 L-Met + 2-oxobutanoate + NADH 11% of the activity with pyruvate Arthrobacter sp. ?
-
 r
1.5.1.28 L-Met + 2-oxohexanoate + NADH 2.1% of the activity with pyruvate Arthrobacter sp. ?
-
 r
1.5.1.28 L-Met + glyoxylate + NADH 11% of the activity with pyruvate Arthrobacter sp. ?
-
 r
1.5.1.28 L-Met + hydroxypyruvate + NADH 6.1% of the activity with pyruvate Arthrobacter sp. ?
-
 r
1.5.1.28 L-Met + oxaloacetate + NADH 30% of the activity with pyruvate Arthrobacter sp. ?
-
 r
1.5.1.28 L-Met + pyruvate + NADH
-
 Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Met + NAD+
-
 r
1.5.1.28 L-Met + pyruvate + NADH
-
 Arthrobacter sp. 1C N-[1-(R)-(Carboxy)ethyl]-(S)-Met + NAD+
-
 r
1.5.1.28 L-Phe + pyruvate + NADH 91% of the activity with L-Met Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Phe + NAD+
-
 ?
1.5.1.28 L-Phe + pyruvate + NADH 91% of the activity with L-Met Arthrobacter sp. 1C N-[1-(R)-(Carboxy)ethyl]-(S)-Phe + NAD+
-
 ?
1.5.1.28 L-Ser + pyruvate + NADH 39% of the activity with L-Met Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Ser + NAD+
-
 r
1.5.1.28 L-Thr + pyruvate + NADH 50% of the activity with L-Met Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Thr + NAD+
-
 r
1.5.1.28 L-Val + pyruvate + NADH 92% of the activity with L-Met Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Val + NAD+
-
 r
1.5.1.28 L-Val + pyruvate + NADH 92% of the activity with L-Met Arthrobacter sp. 1C N-[1-(R)-(Carboxy)ethyl]-(S)-Val + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.5.1.28 dimer
-
 Arthrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.28 55
-
-
 Arthrobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.28 30
-
 pH 5.0-9.5, 1 h, less than 15% loss of activity Arthrobacter sp.
1.5.1.28 37
-
 pH 8.0, 10 min, stable Arthrobacter sp.
1.5.1.28 50
-
 pH 8.0, 10 min, 20% loss of activity Arthrobacter sp.
1.5.1.28 52
-
 pH 8.0, 10 min, 50% loss of activity Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.28 8
-
 amine-forming reaction Arthrobacter sp.
1.5.1.28 10
-
 oxidative deamination Arthrobacter sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.5.1.28 5 9.5 30°C, 1 h less than 15% loss of activity Arthrobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.28 NADH
-
 Arthrobacter sp.