EC Number | Crystallization (Comment) | Organism |
---|---|---|
6.1.1.6 | - |
Geobacillus stearothermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.11 | additional information | - |
additional information | a temperature-dependent inhibition by conformational change of the enzyme (50°C-70°C) lowers both the catalytic activity and the substrate affinity of the ATP and amino acid sites as indicated by a sharp rise in Km with temperature | Geobacillus stearothermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.1.1.6 | 58000 | - |
2 * 58000, SDS-PAGE | Geobacillus stearothermophilus |
6.1.1.6 | 109000 | - |
gel filtration | Geobacillus stearothermophilus |
6.1.1.6 | 112000 | - |
equilibrium sedimentation measurement | Geobacillus stearothermophilus |
6.1.1.11 | 49000 | - |
2 * 49000, SDS-PAGE | Geobacillus stearothermophilus |
6.1.1.11 | 81000 | - |
gel filtration, sedimentation velocity | Geobacillus stearothermophilus |
6.1.1.11 | 88000 | - |
sedimentation equilibrium measurement | Geobacillus stearothermophilus |
6.1.1.22 | 51000 | - |
2 * 51000, sedimentation equilibrium measurement under denaturing conditions | Geobacillus stearothermophilus |
6.1.1.22 | 120000 | - |
calculation from gel filtration and sedimentation velocity | Geobacillus stearothermophilus |
6.1.1.22 | 127000 | - |
sedimentation equilibrium measurement | Geobacillus stearothermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.6 | Geobacillus stearothermophilus | - |
- |
- |
6.1.1.11 | Geobacillus stearothermophilus | - |
- |
- |
6.1.1.22 | Geobacillus stearothermophilus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.1.1.6 | - |
Geobacillus stearothermophilus |
6.1.1.11 | - |
Geobacillus stearothermophilus |
6.1.1.22 | - |
Geobacillus stearothermophilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.1.1.6 | 0.345 | - |
- |
Geobacillus stearothermophilus |
6.1.1.11 | 0.106 | - |
- |
Geobacillus stearothermophilus |
6.1.1.22 | 0.113 | - |
- |
Geobacillus stearothermophilus |
EC Number | Storage Stability | Organism |
---|---|---|
6.1.1.6 | -80°C, 0.1 M potassium phosphate buffer, pH 7.0 | Geobacillus stearothermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.6 | ATP + lysine + tRNALys | - |
Geobacillus stearothermophilus | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
6.1.1.11 | ATP + L-serine + tRNASer | - |
Geobacillus stearothermophilus | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
6.1.1.22 | ATP + L-asparagine + tRNAAsn | - |
Geobacillus stearothermophilus | AMP + diphosphate + L-asparaginyl-tRNAAsn | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.1.1.6 | dimer | 2 * 58000, SDS-PAGE | Geobacillus stearothermophilus |
6.1.1.11 | dimer | 2 * 49000, SDS-PAGE | Geobacillus stearothermophilus |
6.1.1.22 | dimer | 2 * 51000, sedimentation equilibrium measurement under denaturing conditions | Geobacillus stearothermophilus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.1.1.22 | additional information | - |
temperature-induced conformation change of the enzymes affecting primarily the binding sites for ATP and amino acid | Geobacillus stearothermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.1.1.6 | 60 | - |
after 4 h complete loss of aminoacylation activity, 3 min, stable in presence of all substrates except lysine | Geobacillus stearothermophilus |
6.1.1.6 | 70 | - |
0.5 min, stable in presence of all substrates except lysine | Geobacillus stearothermophilus |
6.1.1.22 | 60 | - |
3 min, stable, in presence of all substrates except amino acid | Geobacillus stearothermophilus |
6.1.1.22 | 70 | - |
0.5 min, stable, in presence of all substrates except amino acid | Geobacillus stearothermophilus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
6.1.1.11 | 6 | 8 | 25°C, acylation rate increases when pH is raised from 6.0 to 8.0 | Geobacillus stearothermophilus |
6.1.1.22 | 6 | 8 | 25 C, the aminoacylation rate increases as the pH is raised from 6.0 to 8.0 | Geobacillus stearothermophilus |