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Literature summary extracted from

  • Brockbank, W.J.; Lynn, K.R.
    Purification and preliminary characterization of two asclepains from the latex of Asclepias syriaca L. (milkweed) (1979), Biochim. Biophys. Acta, 578, 13-22.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.7 iodoacetic acid
-
 Asclepias syriaca
3.4.22.7 PCMB
-
 Asclepias syriaca
3.4.22.7 Sodium tetrathionate
-
 Asclepias syriaca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.7 19000
-
 asclepain B5, gel filtration Asclepias syriaca
3.4.22.7 23000
-
 1 * 23000, carboxymethylated asclepain A3 and carboxymethylated asclepain B5, SDS-PAGE Asclepias syriaca
3.4.22.7 24000
-
 asclepain A3, gel filtration Asclepias syriaca

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.7 Asclepias syriaca
-
 asclepain A3 and B5
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.7 no modification no bound carbohydrate Asclepias syriaca

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.7
-
 Asclepias syriaca

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.7 latex
-
 Asclepias syriaca
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.7 4100
-
 asclepain A3 Asclepias syriaca
3.4.22.7 6300
-
 ascepain B5 Asclepias syriaca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.7 benzoyl-L-Arg-p-nitroanilide + H2O
-
 Asclepias syriaca benzoyl-L-Arg + 4-nitroaniline
-
 ?
3.4.22.7 casein + H2O
-
 Asclepias syriaca ?
-
 ?
3.4.22.7 N-carbobenzoxy-Gly p-nitrophenyl ester + H2O
-
 Asclepias syriaca N-carbobenzoxy-Gly + p-nitrophenol
-
 ?
3.4.22.7 N-carbobenzoxy-Leu p-nitrophenyl ester + H2O
-
 Asclepias syriaca N-carbobenzoxy-Leu + p-nitrophenol
-
 ?
3.4.22.7 N-carbobenzoxy-Tyr p-nitrophenyl ester + H2O
-
 Asclepias syriaca N-carbobenzoxy-Tyr + p-nitrophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.7 monomer 1 * 23000, carboxymethylated asclepain A3 and carboxymethylated asclepain B5, SDS-PAGE Asclepias syriaca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.7 7 7.5 hydrolysis of casein, asclepain B5 Asclepias syriaca
3.4.22.7 7.5 8.5 hydrolysis of casein, asclepain A3 Asclepias syriaca