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Literature summary extracted from

  • McDowall, M.A.
    Anionic proteinase from Actinidia chinensis. Preparation and properties of the crystalline enzyme (1970), Eur. J. Biochem., 14, 214-221.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.22.14
-
 Actinidia chinensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.14 4,4'-dithiodipyridine
-
 Actinidia chinensis
3.4.22.14 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Actinidia chinensis
3.4.22.14 iodoacetate
-
 Actinidia chinensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.14 89
-
 benzoyl-L-Arg ethyl ester pH 5.6, 25°C, in 0.3 M KCl Actinidia chinensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.14 12800
-
 gel filtration Actinidia chinensis

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.14 Actinidia chinensis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.14 fruit
-
 Actinidia chinensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.14 additional information
-
-
 Actinidia chinensis

Storage Stability

EC Number Storage Stability Organism
3.4.22.14 4°C, crystalline enzyme, resuspended in dialysis buffer at pH 4, stable for several months Actinidia chinensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.14 Benzoyl-L-Arg ethyl ester + H2O
-
 Actinidia chinensis Benzoyl-L-Arg + ethanol
-
 ?
3.4.22.14 Gelatin + H2O
-
 Actinidia chinensis ?
-
 ?
3.4.22.14 p-toluene-sulphonyl-L-glutamine p-nitrophenyl ester + H2O
-
 Actinidia chinensis p-toluene-sulphonyl-L-glutamine + 4-nitrophenol
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.14 2.6
-
 benzoyl-L-Arg ethyl ester pH 5.6, 25°C, in 0.3 M KCl Actinidia chinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.14 5 6
-
 Actinidia chinensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.14 3.5 8.8 about 45% of maximal activity at pH 3.5 and at pH 8.8 Actinidia chinensis