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Literature summary extracted from
- Streptococcal proteinase (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 3, 609-647.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.10 | dithiothreitol | reduction is essential for catalytic activity | Streptococcus sp. |  |
| 3.4.22.10 | thioethanol | reduction is essential for catalytic activity, once the enzyme is activated by the reducing agent, the presence of the activator is no longer necessary for catalytic activity | Streptococcus sp. | |
| 3.4.22.10 | thioglycolic acid | reduction is essential for catalytic activity | Streptococcus sp. | |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.4.22.10 | - |
Streptococcus sp. |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.22.10 | 0.1 N HCl or 0.01 N NaOH, room temperature, 2 h, denaturation | Streptococcus sp. |
| 3.4.22.10 | 0.1 N HCl, 2 h, denaturation of the zymogen | Streptococcus sp. |
| 3.4.22.10 | 0.5% SDS, denaturation | Streptococcus sp. |
| 3.4.22.10 | 8 M urea plus reducing agent, 20% of proteolytic activity is provided | Streptococcus sp. |
| 3.4.22.10 | EDTA, 0.001 M, stabilizes | Streptococcus sp. |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.10 | 2,4-dinitrofluorobenzene | - |
Streptococcus sp. | |
| 3.4.22.10 | Benzyloxycarbonyl-Gly-Phe | competitive against benzyloxycarbonyl-Phe-Tyr | Streptococcus sp. | |
| 3.4.22.10 | iodoacetamide | - |
Streptococcus sp. | |
| 3.4.22.10 | iodoacetic acid | - |
Streptococcus sp. | |
| 3.4.22.10 | methyliodine | - |
Streptococcus sp. | |
| 3.4.22.10 | NEM | - |
Streptococcus sp. | |
| 3.4.22.10 | Sodium tetrathionate | - |
Streptococcus sp. | |
| 3.4.22.10 | Tosyl-norleucine-Phe | competitive against benzyloxycarbonyl-norleucine-Phe | Streptococcus sp. | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.22.10 | additional information | - |
additional information | effect of pH-value on the Km-value for benzyloxycarbonyl-Lys-p-nitrophenyl ester | Streptococcus sp. | |
| 3.4.22.10 | 0.22 | - |
benzyloxycarbonyl-Lys-p-nitrophenyl ester | pH 4.27 | Streptococcus sp. | |
| 3.4.22.10 | 0.262 | - |
benzyloxycarbonyl-Lys-p-nitrophenyl ester | pH 6.35 | Streptococcus sp. | |
| 3.4.22.10 | 0.494 | - |
benzyloxycarbonyl-Lys-p-nitrophenyl ester | pH 4.49 | Streptococcus sp. | |
| 3.4.22.10 | 2.28 | - |
benzyloxycarbonyl-Lys-Phe | - |
Streptococcus sp. | |
| 3.4.22.10 | 2.64 | - |
Nalpha-benzyloxycarbonyl-Nepsilon-t-butyloxycarbonyl-Lys-Phe | - |
Streptococcus sp. | |
| 3.4.22.10 | 3.88 | - |
benzyloxycarbonyl-Glu-Phe | - |
Streptococcus sp. | |
| 3.4.22.10 | 5.05 | - |
Nalpha-benzyloxycarbonyl-Lys-Phe | - |
Streptococcus sp. | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.10 | bisulfite | reduction is essential for catalytic activity | Streptococcus sp. | |
| 3.4.22.10 | cyanide | reduction is essential for catalytic activity | Streptococcus sp. | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.10 | 32000 | - |
high-speed equilibrium ultracentrifugation | Streptococcus sp. |
| 3.4.22.10 | 33590 | - |
determination of amino acid composition | Streptococcus sp. |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.10 | Streptococcus sp. | - |
hemolytic | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.22.10 | side-chain modification | - |
Streptococcus sp. |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 3.4.22.10 | 90% renaturation provided the denatured zymogen is solubilized with urea or guanidinium chloride and given time to refold | Streptococcus sp. |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.22.10 | -20°C, stable for at least 2 years | Streptococcus sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.10 | benzyloxycarbonyl-Glu-p-nitrophenyl ester + H2O | - |
Streptococcus sp. | ? | - |
? | |
| 3.4.22.10 | benzyloxycarbonyl-Glu-Phe + H2O | - |
Streptococcus sp. | ? | - |
? | |
| 3.4.22.10 | benzyloxycarbonyl-Gly-p-nitrophenyl ester + H2O | - |
Streptococcus sp. | benzyloxycarbonyl-Gly + p-nitrophenol | - |
? | |
| 3.4.22.10 | benzyloxycarbonyl-Nle-p-nitrophenyl ester + H2O | - |
Streptococcus sp. | ? | - |
? | |
| 3.4.22.10 | benzyloxycarbonyl-Nle-Phe + H2O | - |
Streptococcus sp. | ? | - |
? | |
| 3.4.22.10 | N-benzyloxycarbonyl-Gly-p-nitrophenyl ester + H2O | - |
Streptococcus sp. | N-benzyloxycarbonyl-Gly + 4-nitrophenol | - |
? | |
| 3.4.22.10 | Nalpha-benzyloxycarbonyl-Lys-Phe + H2O | - |
Streptococcus sp. | ? | - |
? | |
| 3.4.22.10 | Nalpha-benzyloxycarbonyl-Nepsilon-t-butyloxycarbonyl-Lys-Phe + H2O | - |
Streptococcus sp. | ? | - |
? | |
| 3.4.22.10 | Nalpha-benzyloxycarbonyl-Nepsilon-tosyl-Lys-p-nitrophenyl ester + H2O | - |
Streptococcus sp. | Nalpha-benzyloxycarbonyl-Nepsilon-tosyl-Lys + p-nitrophenol | - |
? | |
| 3.4.22.10 | Nalpha-benzyloxycarbonyl-Phe-Tyr + H2O | - |
Streptococcus sp. | ? | - |
? | |
| 3.4.22.10 | Nepsilon-benzyloxycarbonyl-Lys-p-nitrophenyl ester + H2O | - |
Streptococcus sp. | Nepsilon-benzyloxycarbonyl-Lys + 4-nitrophenol | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.10 | 25 | - |
0.1 N HCl, 2 h, denaturation of the zymogen | Streptococcus sp. |
| 3.4.22.10 | 60 | - |
irreversible inactivation | Streptococcus sp. |
| 3.4.22.10 | 100 | - |
pH 6.7, 10 min, denaturation of the zymogen | Streptococcus sp. |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.22.10 | additional information | - |
additional information | effect of pH-value on the turnover numer for benzyloxycarbonyl-Lys-p-nitrophenyl ester | Streptococcus sp. | |
| 3.4.22.10 | 1.09 | - |
benzyloxycarbonyl-Lys-Phe | - |
Streptococcus sp. | |
| 3.4.22.10 | 2.3 | - |
benzyloxycarbonyl-Glu-Phe | - |
Streptococcus sp. | |
| 3.4.22.10 | 5.05 | - |
Nalpha-benzyloxycarbonyl-Lys-Phe | - |
Streptococcus sp. | |
| 3.4.22.10 | 8.6 | - |
Nalpha-benzyloxycarbonyl-Nepsilon-t-butyloxycarbonyl-Lys-Phe | - |
Streptococcus sp. | |
| 3.4.22.10 | 29.7 | - |
benzyloxycarbonyl-Lys-p-nitrophenyl ester | pH 4.27 | Streptococcus sp. | |
| 3.4.22.10 | 89.8 | - |
benzyloxycarbonyl-Lys-p-nitrophenyl ester | pH 4.49 | Streptococcus sp. | |
| 3.4.22.10 | 142 | - |
benzyloxycarbonyl-Lys-p-nitrophenyl ester | pH 6.35 | Streptococcus sp. | |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.10 | 4.5 | 9.5 | unstable below pH 4.5 and above pH 9.5 | Streptococcus sp. |
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