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Literature summary extracted from

  • Yoshimoto, T.; Simmons, W.H.; Kita, T.; Tsuru, D.
    Post-proline cleaving enzyme from lamb brain (1981), J. Biochem., 90, 325-334.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.26 4-Chloromercuriphenylsulfonate weak Ovis aries
3.4.21.26 Chloromethylketone derivatives
-
 Ovis aries
3.4.21.26 Cu2+
-
 Ovis aries
3.4.21.26 diisopropylfluorophosphate
-
 Ovis aries
3.4.21.26 Hg2+
-
 Ovis aries
3.4.21.26 iodoacetate weak Ovis aries
3.4.21.26 p-chloromercuribenzoate
-
 Ovis aries
3.4.21.26 Pancreatic basic trypsin inhibitor
-
 Ovis aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.26 additional information
-
 additional information
-
 Ovis aries
3.4.21.26 0.48
-
 N-benzyloxycarbonyl-Gly-Pro-Ala
-
 Ovis aries

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.26 72000 74000 gel filtration Ovis aries
3.4.21.26 77000
-
 ? * 77000, SDS-PAGE Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.26 Ovis aries
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.26
-
 Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.26 brain
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.26 additional information
-
-
 Ovis aries

Storage Stability

EC Number Storage Stability Organism
3.4.21.26 4°C, 50% loss of activity after 1 week Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.26 benzyloxycarbonyl-glycyl-proline-p-nitroanilide + H2O
-
 Ovis aries ?
-
 ?
3.4.21.26 N-benzyloxycarbonyl-Gly-Pro-Ala + H2O
-
 Ovis aries ?
-
 ?
3.4.21.26 Nalpha-benzyloxycarbonyl-Gly-Pro-Leu-Gly + H2O
-
 Ovis aries ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.26 More ? * 77000, SDS-PAGE Ovis aries

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.26 45
-
-
 Ovis aries

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.26 20 25 12 h, 50% loss of activity Ovis aries

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.26 additional information
-
 additional information
-
 Ovis aries
3.4.21.26 6.1
-
 N-benzyloxycarbonyl-Gly-Pro-Ala
-
 Ovis aries
3.4.21.26 32.3
-
 N-benzyloxycarbonyl-Gly-Pro-Leu-Gly
-
 Ovis aries

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.26 7
-
-
 Ovis aries
3.4.21.26 7.2 7.5 benzyloxycarbonylglycyl-proline-p-nitroanilide Ovis aries
3.4.21.26 7.8 8 benzyloxycarbonylglycycl-prolyl-leucyl-glycinamide, benzyloxycarbonyl-glycyl-proline beta-naphthylamide Ovis aries

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.26 5.5 9 30°C, 1 h Ovis aries