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Literature summary extracted from

  • Kakudo, S.; Kikuchi, N.; Kitadokoro, K.; Fujiwaera, T.; Nakamura, E.; Okamoto, H.; Shin, M.; Tamaki, M.; Teraoka, H.; Tsuzuki, H.; Yoshida, N.
    Purification, characterization, cloning, and expression of a glutamic acid-specific protease from Bacillus licheniformis ATCC 14580 (1992), J. Biol. Chem., 267, 23782-23788.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.19
-
 Bacillus licheniformis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.21.19
-
 Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.19 0.5
-
 benzyloxycarbonyl-Leu-Leu-Glu-p-nitroanilide
-
 Staphylococcus aureus
3.4.21.19 1.2
-
 benzyloxycarbonyl-Phe-Leu-Glu-p-nitroanilide
-
 Staphylococcus aureus
3.4.21.19 1.5
-
 benzyloxycarbonyl-Ala-Leu-Glu-p-nitroanilide
-
 Bacillus licheniformis
3.4.21.19 1.8
-
 benzyloxycarbonyl-Glu-p-nitroanilide
-
 Bacillus licheniformis
3.4.21.19 2.8
-
 benzyloxycarbonyl-Leu-Leu-Glu-p-nitroanilide
-
 Bacillus licheniformis
3.4.21.19 3.5
-
 benzyloxycarbonyl-Ala-Ala-Glu-4-nitroanilide
-
 Bacillus licheniformis
3.4.21.19 3.9
-
 benzyloxycarbonyl-Ala-Leu-Glu-p-nitroanilide
-
 Staphylococcus aureus
3.4.21.19 3.9
-
 benzyloxycarbonyl-Phe-Leu-Glu-p-nitroanilide
-
 Bacillus licheniformis
3.4.21.19 4.2
-
 benzyloxycarbonyl-Ala-Glu-p-nitroanilide
-
 Bacillus licheniformis
3.4.21.19 4.9
-
 t-butyloxycarbonyl-Ala-Ala-Glu-p-nitroanilide
-
 Bacillus licheniformis
3.4.21.19 5.5
-
 benzyloxycarbonyl-Leu-Glu-p-nitroanilide
-
 Bacillus licheniformis
3.4.21.19 7.6
-
 benzyloxycarbonyl-Ala-Ala-Glu-4-nitroanilide
-
 Staphylococcus aureus
3.4.21.19 11.6
-
 t-butyloxycarbonyl-Ala-Ala-Asp-p-nitroanilide
-
 Bacillus licheniformis
3.4.21.19 13.3
-
 benzyloxycarbonyl-Ala-Glu-p-nitroanilide
-
 Staphylococcus aureus
3.4.21.19 16.5
-
 benzyloxycarbonyl-Leu-Glu-p-nitroanilide
-
 Staphylococcus aureus
3.4.21.19 19.7
-
 t-butyloxycarbonyl-Ala-Ala-Glu-p-nitroanilide
-
 Staphylococcus aureus
3.4.21.19 24.5
-
 t-butyloxycarbonyl-Ala-Ala-Asp-p-nitroanilide
-
 Staphylococcus aureus
3.4.21.19 28.4
-
 benzyloxycarbonyl-Glu-p-nitroanilide
-
 Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.19 25000
-
 x * 25000, SDS-PAGE Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.19 Bacillus licheniformis
-
 ATCC 14580
-
3.4.21.19 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.19
-
 Bacillus licheniformis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.19 additional information
-
-
 Staphylococcus aureus
3.4.21.19 additional information
-
-
 Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.19 acetyl-Asp-p-nitroanilide + H2O
-
 Staphylococcus aureus acetyl-Glu + p-nitroaniline
-
 ?
3.4.21.19 acetyl-Asp-p-nitroanilide + H2O
-
 Bacillus licheniformis acetyl-Glu + p-nitroaniline
-
 ?
3.4.21.19 acetyl-Glu-p-nitroanilide + H2O
-
 Staphylococcus aureus acetyl-Glu + p-nitroaniline
-
 ?
3.4.21.19 acetyl-Glu-p-nitroanilide + H2O
-
 Bacillus licheniformis acetyl-Glu + p-nitroaniline
-
 ?
3.4.21.19 benzyloxycarbonyl-Ala-Ala-Glu-4-nitroanilide + H2O
-
 Staphylococcus aureus benzyloxycarbonyl-Ala-Ala-Glu + 4-nitroaniline
-
 ?
3.4.21.19 benzyloxycarbonyl-Ala-Ala-Glu-4-nitroanilide + H2O
-
 Bacillus licheniformis benzyloxycarbonyl-Ala-Ala-Glu + 4-nitroaniline
-
 ?
3.4.21.19 benzyloxycarbonyl-Ala-Glu-p-nitroanilide + H2O
-
 Staphylococcus aureus benzyloxycarbonyl-Ala-Glu + p-nitroaniline
-
 ?
3.4.21.19 benzyloxycarbonyl-Ala-Glu-p-nitroanilide + H2O
-
 Bacillus licheniformis benzyloxycarbonyl-Ala-Glu + p-nitroaniline
-
 ?
3.4.21.19 benzyloxycarbonyl-Ala-Leu-Glu-p-nitroanilide + H2O
-
 Staphylococcus aureus benzyloxycarbonyl-Ala-Leu-Glu + p-nitroaniline
-
 ?
3.4.21.19 benzyloxycarbonyl-Ala-Leu-Glu-p-nitroanilide + H2O
-
 Bacillus licheniformis benzyloxycarbonyl-Ala-Leu-Glu + p-nitroaniline
-
 ?
3.4.21.19 benzyloxycarbonyl-Glu-p-nitroanilide + H2O
-
 Staphylococcus aureus benzyloxycarbonyl-Glu + p-nitroaniline
-
 ?
3.4.21.19 benzyloxycarbonyl-Glu-p-nitroanilide + H2O
-
 Bacillus licheniformis benzyloxycarbonyl-Glu + p-nitroaniline
-
 ?
3.4.21.19 benzyloxycarbonyl-Leu-Glu-p-nitroanilide + H2O
-
 Staphylococcus aureus benzyloxycarbonyl-Leu-Glu + p-nitroaniline
-
 ?
3.4.21.19 benzyloxycarbonyl-Leu-Glu-p-nitroanilide + H2O
-
 Bacillus licheniformis benzyloxycarbonyl-Leu-Glu + p-nitroaniline
-
 ?
3.4.21.19 benzyloxycarbonyl-Leu-Leu-Glu-p-nitroanilide + H2O
-
 Staphylococcus aureus benzyloxycarbonyl-Leu-Leu-Glu + p-nitroaniline
-
 ?
3.4.21.19 benzyloxycarbonyl-Leu-Leu-Glu-p-nitroanilide + H2O
-
 Bacillus licheniformis benzyloxycarbonyl-Leu-Leu-Glu + p-nitroaniline
-
 ?
3.4.21.19 benzyloxycarbonyl-Phe-Leu-Glu-p-nitroanilide + H2O
-
 Staphylococcus aureus benzyloxycarbonyl-Phe-Leu-Glu + p-nitroaniline
-
 ?
3.4.21.19 benzyloxycarbonyl-Phe-Leu-Glu-p-nitroanilide + H2O
-
 Bacillus licheniformis benzyloxycarbonyl-Phe-Leu-Glu + p-nitroaniline
-
 ?
3.4.21.19 human parathyroid hormone(13-34) + H2O
-
 Staphylococcus aureus peptides peptide fragments Lys1-Glu6, Arg8-Glu10 and Lys1-Glu10 are produced after 6 min ?
3.4.21.19 human parathyroid hormone(13-34) + H2O
-
 Bacillus licheniformis peptides peptide fragments Lys1-Glu7, Arg8-Glu10 and Trp11-Phe22 are produced after 6 min ?
3.4.21.19 t-butyloxycarbonyl-Ala-Ala-Asp-p-nitroanilide + H2O
-
 Staphylococcus aureus t-butyloxycarbonyl-Ala-Ala-Asp + p-nitroaniline
-
 ?
3.4.21.19 t-butyloxycarbonyl-Ala-Ala-Asp-p-nitroanilide + H2O
-
 Bacillus licheniformis t-butyloxycarbonyl-Ala-Ala-Asp + p-nitroaniline
-
 ?
3.4.21.19 t-butyloxycarbonyl-Ala-Ala-Glu-p-nitroanilide + H2O
-
 Staphylococcus aureus t-butyloxycarbonyl-Ala-Ala-Glu + p-nitroaniline
-
 ?
3.4.21.19 t-butyloxycarbonyl-Ala-Ala-Glu-p-nitroanilide + H2O
-
 Bacillus licheniformis t-butyloxycarbonyl-Ala-Ala-Glu + p-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.19 ? x * 25000, SDS-PAGE Bacillus licheniformis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.19 0.16
-
 t-butyloxycarbonyl-Ala-Ala-Asp-p-nitroanilide
-
 Bacillus licheniformis
3.4.21.19 0.28
-
 benzyloxycarbonyl-Glu-p-nitroanilide
-
 Bacillus licheniformis
3.4.21.19 0.29
-
 benzyloxycarbonyl-Glu-p-nitroanilide
-
 Staphylococcus aureus
3.4.21.19 0.54
-
 t-butyloxycarbonyl-Ala-Ala-Asp-p-nitroanilide
-
 Staphylococcus aureus
3.4.21.19 5.6
-
 benzyloxycarbonyl-Ala-Glu-p-nitroanilide
-
 Staphylococcus aureus
3.4.21.19 5.9
-
 benzyloxycarbonyl-Ala-Glu-p-nitroanilide
-
 Bacillus licheniformis
3.4.21.19 11.9
-
 t-butyloxycarbonyl-Ala-Ala-Glu-p-nitroanilide
-
 Bacillus licheniformis
3.4.21.19 20.8
-
 benzyloxycarbonyl-Ala-Ala-Glu-4-nitroanilide
-
 Staphylococcus aureus
3.4.21.19 22.6
-
 benzyloxycarbonyl-Leu-Glu-p-nitroanilide
-
 Bacillus licheniformis
3.4.21.19 24
-
 t-butyloxycarbonyl-Ala-Ala-Glu-p-nitroanilide
-
 Staphylococcus aureus
3.4.21.19 30.2
-
 benzyloxycarbonyl-Leu-Leu-Glu-p-nitroanilide
-
 Staphylococcus aureus
3.4.21.19 31.4
-
 benzyloxycarbonyl-Leu-Glu-p-nitroanilide
-
 Staphylococcus aureus
3.4.21.19 33.2
-
 benzyloxycarbonyl-Phe-Leu-Glu-p-nitroanilide
-
 Staphylococcus aureus
3.4.21.19 40.9
-
 benzyloxycarbonyl-Phe-Leu-Glu-p-nitroanilide
-
 Bacillus licheniformis
3.4.21.19 40.9
-
 benzyloxycarbonyl-Ala-Ala-Glu-4-nitroanilide
-
 Bacillus licheniformis
3.4.21.19 50.8
-
 benzyloxycarbonyl-Leu-Leu-Glu-p-nitroanilide
-
 Bacillus licheniformis
3.4.21.19 60.9
-
 benzyloxycarbonyl-Ala-Leu-Glu-p-nitroanilide
-
 Bacillus licheniformis
3.4.21.19 131
-
 benzyloxycarbonyl-Ala-Leu-Glu-p-nitroanilide
-
 Bacillus licheniformis