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Literature summary extracted from
- Bacterial peptidases. 3. An enzyme specific for N-acyl linkages to alanine (1969), J. Biol. Chem., 244, 4467-4472.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.17.6 | Benzoyl-Ala | at least 30% inhibition at 0.3 mM; competitive, Ki: 0.009 mM | Soil bacterium |  |
| 3.4.17.6 | benzoyl-Leu-Ala | at least 30% inhibition at 0.3 mM | Soil bacterium | |
| 3.4.17.6 | carboxyl-terminal Ala containing compounds | - |
Soil bacterium | |
| 3.4.17.6 | Gly-Ala | non-competitive, Ki: 0.13 mM | Soil bacterium | |
| 3.4.17.6 | His-Ala | non-competitive, Ki: 0.34 mM | Soil bacterium | |
| 3.4.17.6 | additional information | not: compounds containing carboxyl-terminal Met or Gly | Soil bacterium | |
| 3.4.17.6 | Phe-Ala | at least 30% inhibition at 0.3 mM | Soil bacterium | |
| 3.4.17.6 | phosphate buffer | - |
Soil bacterium | |
| 3.4.17.6 | Tyr-Ala | at least 30% inhibition at 0.3 mM | Soil bacterium | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.17.6 | 4.3 | - |
N-benzoyl-L-aminobutyric acid | - |
Soil bacterium | |
| 3.4.17.6 | 4.3 | - |
N-Benzoyl-Ala | - |
Soil bacterium | |
| 3.4.17.6 | 6.7 | - |
N-benzoyl-Gly | - |
Soil bacterium | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.17.6 | additional information | no effect: Co2+, Mn2+, Mg2+, Fe3+, Fe2+, Cu2+, Cd2+, Ni2+ | Soil bacterium | |
| 3.4.17.6 | Zn2+ | required | Soil bacterium | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.17.6 | 4-aminobenzoylalanine + H2O | Soil bacterium | - |
? | - |
? | |
| 3.4.17.6 | peptidyl-L-alanine + H2O | Soil bacterium | peptide can be replaced by a variety of pteroyl or acyl groups, absolute specificity for alanine in carboxyl-terminal position | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.17.6 | Soil bacterium | - |
unidentified | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.17.6 | - |
Soil bacterium |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.6 | 8.3 | - |
- |
Soil bacterium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.17.6 | 4-amino-4-deoxypteroyl-Ala + H2O | - |
Soil bacterium | 4-amino-4-deoxypteroate + Ala | - |
ir | |
| 3.4.17.6 | 4-aminobenzoyl-Ala + H2O | - |
Soil bacterium | 4-aminobenzoate + Ala | - |
ir | |
| 3.4.17.6 | 4-aminobenzoylalanine + H2O | - |
Soil bacterium | ? | - |
? | |
| 3.4.17.6 | acyl-Ala + H2O | - |
Soil bacterium | ? + Ala | - |
ir | |
| 3.4.17.6 | Ala-Ala + H2O | - |
Soil bacterium | Ala + Ala | - |
ir | |
| 3.4.17.6 | benzoyl-Ala + H2O | - |
Soil bacterium | benzoate + Ala | - |
ir | |
| 3.4.17.6 | benzyloxycarbonyl-Ala + H2O | - |
Soil bacterium | benzyloxy-CHO + Ala | - |
ir | |
| 3.4.17.6 | benzyloxycarbonyl-Ala-Ala + H2O | - |
Soil bacterium | benzyloxycarbonyl-Ala + Ala | - |
ir | |
| 3.4.17.6 | benzyloxycarbonyl-Asp-Ala + H2O | - |
Soil bacterium | benzyloxycarbonyl-Asp + Ala | - |
ir | |
| 3.4.17.6 | benzyloxycarbonyl-Ile-Ala + H2O | - |
Soil bacterium | benzyloxycarbonyl-Ile + Ala | - |
ir | |
| 3.4.17.6 | benzyloxycarbonyl-Leu-Ala + H2O | - |
Soil bacterium | benzyloxycarbonyl-Leu + Ala | - |
ir | |
| 3.4.17.6 | benzyloxycarbonyl-Met-Ala + H2O | - |
Soil bacterium | benzyloxycarbonyl-Met + Ala | - |
ir | |
| 3.4.17.6 | benzyloxycarbonyl-Phe-Ala + H2O | - |
Soil bacterium | benzyloxycarbonyl-Phe + Ala | - |
ir | |
| 3.4.17.6 | benzyloxycarbonyl-Ser-Ala + H2O | - |
Soil bacterium | benzyloxycarbonyl-Ser + Ala | - |
ir | |
| 3.4.17.6 | Glu-Ala + H2O | - |
Soil bacterium | Glu + Ala | - |
ir | |
| 3.4.17.6 | Ile-Ala + H2O | - |
Soil bacterium | Ile + Ala | - |
ir | |
| 3.4.17.6 | Leu-Ala + H2O | - |
Soil bacterium | Leu + Ala | - |
ir | |
| 3.4.17.6 | Met-Ala + H2O | - |
Soil bacterium | Met + Ala | - |
ir | |
| 3.4.17.6 | N-benzoyl-Gly-Ala + H2O | - |
Soil bacterium | N-benzoyl-Gly + Ala | - |
? | |
| 3.4.17.6 | N-benzoyl-L-aminobutyric acid + H2O | - |
Soil bacterium | benzoate + L-aminobutyric acid | - |
? | |
| 3.4.17.6 | peptidyl-L-alanine + H2O | peptide can be replaced by a variety of pteroyl or acyl groups, absolute specificity for alanine in carboxyl-terminal position | Soil bacterium | ? | - |
? | |
| 3.4.17.6 | Phe-Ala + H2O | - |
Soil bacterium | Phe + Ala | - |
ir | |
| 3.4.17.6 | Ser-Ala + H2O | - |
Soil bacterium | Ser + Ala | - |
ir | |
| 3.4.17.6 | Tyr-Ala + H2O | - |
Soil bacterium | Tyr + Ala | - |
ir | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.6 | 30 | - |
assay at | Soil bacterium |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.6 | 7 | 8 | - |
Soil bacterium |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.17.6 | 0.009 | - |
Benzoyl-Ala | - |
Soil bacterium | |
| 3.4.17.6 | 0.13 | - |
Gly-Ala | non-competitive | Soil bacterium | |
| 3.4.17.6 | 0.34 | - |
His-Ala | non-competitive | Soil bacterium | |
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Release 2023.1 (January 2023)
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