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Literature summary extracted from

  • Brown, J.L.
    Purification and properties of dipeptidase M from Escherichia coli B (1973), J. Biol. Chem., 248, 409-416.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.13.12 generally unstable Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.13.12 EDTA Mn2+ may restore enzyme activity after EDTA treatment Escherichia coli
3.4.13.12 iodoacetate 100% inhibition at 10 mM, 10 mM mercaptoethanol restores activity Escherichia coli
3.4.13.12 p-chloromercuribenzoate 100% inhibition at 0.1 mM Escherichia coli
3.4.13.12 Zn2+
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.13.12 0.13
-
 Met-Val
-
 Escherichia coli
3.4.13.12 0.25 0.31 Met-Ala
-
 Escherichia coli
3.4.13.12 0.42 1.02 Met-Ile
-
 Escherichia coli
3.4.13.12 0.45
-
 Met-Leu
-
 Escherichia coli
3.4.13.12 0.53
-
 Met-Thr
-
 Escherichia coli
3.4.13.12 0.57 0.6 Met-Ser
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.13.12 Mn2+ also protects against heat inactivation Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.13.12 47000
-
 2 * 47000, SDS-PAGE Escherichia coli
3.4.13.12 93000 95000 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.13.12 Met-amino acid + H2O Escherichia coli
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.12 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.13.12
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.13.12 1100
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.4.13.12 4°C, 10 mM imidazole buffer, pH 7.2, 2 mg/ml bovine serum albumin, 30 days, 50% loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.12 Ala-Met + H2O
-
 Escherichia coli Ala + Met
-
 ir
3.4.13.12 Gly-Gly + H2O
-
 Escherichia coli Gly + Gly
-
 ir
3.4.13.12 Leu-Ala + H2O
-
 Escherichia coli Leu + Ala
-
 ir
3.4.13.12 Met-Ala + H2O
-
 Escherichia coli Met + Ala
-
 ir
3.4.13.12 Met-amino acid + H2O
-
 Escherichia coli ?
-
 ?
3.4.13.12 Met-Gly + H2O
-
 Escherichia coli Met + Gly
-
 ir
3.4.13.12 Met-Ile + H2O
-
 Escherichia coli Met + Ile
-
 ir
3.4.13.12 Met-Leu + H2O
-
 Escherichia coli Met + Leu
-
 ir
3.4.13.12 Met-Ser + H2O
-
 Escherichia coli Met + Ser
-
 ir
3.4.13.12 Met-Thr + H2O
-
 Escherichia coli Met + Thr
-
 ir
3.4.13.12 Met-Val + H2O
-
 Escherichia coli Met + Val
-
 ?
3.4.13.12 Ser-Ala + H2O
-
 Escherichia coli Ser + Ala
-
 ir
3.4.13.12 Thr-Ala + H2O
-
 Escherichia coli Thr + Ala
-
 ir

Subunits

EC Number Subunits Comment Organism
3.4.13.12 dimer 2 * 47000, SDS-PAGE Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.13.12 50
-
 below, 30 min, Mn2+ protects against heat inactivation Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.13.12 7.8 9 substrate Met-Ala Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.13.12 5.5 9.5 substrate Met-Ala Escherichia coli