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Literature summary extracted from
- Discovery of an epidermal stearoyl-acyl carrier protein thioesterase. Its potential role in wax biosynthesis (1995), J. Biol. Chem., 270, 16962-16969.
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.14 | 40000 | - |
2 * 40000, enzyme from leaf epidermis, SDS-PAGE | Allium ampeloprasum |
| 3.1.2.14 | 84000 | - |
enzyme from leaf epidermis, gel filtration | Allium ampeloprasum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.2.14 | stearoyl-[acyl-carrier protein] + H2O | Allium ampeloprasum | synthesis of fatty acids for wax biosynthesis | ? | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.2.14 | Allium ampeloprasum | - |
- |
- |
| 3.1.2.14 | Brassica rapa subsp. oleifera | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.2.14 | from leaf epidermis | Allium ampeloprasum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.2.14 | leaf epidermis | stearoyl-ACP thioestersae is predominantly expressed in epidermis | Allium ampeloprasum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.14 | 0.144 | - |
enzyme from leaf epidermis | Allium ampeloprasum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.2.14 | stearoyl-[acyl-carrier protein] + H2O | highly specific for, leaf epidermis enzyme | Allium ampeloprasum | stearic acid + acyl-carrier protein | - |
? | |
| 3.1.2.14 | stearoyl-[acyl-carrier protein] + H2O | synthesis of fatty acids for wax biosynthesis | Allium ampeloprasum | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.2.14 | dimer | 2 * 40000, enzyme from leaf epidermis, SDS-PAGE | Allium ampeloprasum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.14 | 8.5 | - |
enzyme from leaf epidermis | Allium ampeloprasum |
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