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Literature summary extracted from

  • Uotila, L.; Koivusalo, M.
    Purification of formaldehyde and formate dehydrogenases from pea seeds by affinity chromatography and S-formylglutathione as the intermediate of formaldehyde metabolism (1979), Arch. Biochem. Biophys., 196, 33-45.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.284 enzyme is unstable in purified state Pisum sativum
1.1.1.284 freezing almost completely destroys activity Pisum sativum
1.17.1.9 rapid freezing in liquid nitrogen gives 75% recovery Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.284 additional information
-
 additional information
-
 Pisum sativum
1.17.1.9 0.45
-
 S-formylglutathione
-
 Pisum sativum
1.17.1.9 2.1
-
 formate
-
 Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.284 42100
-
 2 * 42100, SDS-PAGE Pisum sativum
1.1.1.284 82300
-
 gel filtration Pisum sativum
1.17.1.9 42400
-
 2 * 42400, SDS-PAGE Pisum sativum
1.17.1.9 80300
-
 gel filtration Pisum sativum
1.17.1.9 82300
-
 gel filtration Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.284 Pisum sativum
-
-
-
1.17.1.9 Pisum sativum
-
-
-
3.1.2.12 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.284
-
 Pisum sativum
1.17.1.9
-
 Pisum sativum
3.1.2.12 partial Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.284 seed
-
 Pisum sativum
-
1.17.1.9 seed
-
 Pisum sativum
-
3.1.2.12 seed
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.284 2.78
-
-
 Pisum sativum
1.17.1.9 4.09
-
-
 Pisum sativum

Storage Stability

EC Number Storage Stability Organism
1.1.1.284 0°C, half-life is about 10 days Pisum sativum
1.17.1.9 -70°C, without stabilizer, 85% loss of activity, 7% loss of activity in 33% glycerol solution Pisum sativum
1.17.1.9 0°C, 50% loss of activity after 1 month Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.284 formaldehyde + glutathione + NAD+
-
 Pisum sativum S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 glyoxal + glutathione + NAD+ 35% of the activity with formaldehyde Pisum sativum S-oxoacetylglutathione + NADH
-
 ?
1.1.1.284 methylglyoxal + glutathione + NAD+ 90% of the activity with formaldehyde Pisum sativum S-pyruvylglutathione + NADH
-
 ?
1.17.1.9 ethyl formate + NAD+
-
 Pisum sativum ?
-
 ?
1.17.1.9 formate + NAD+
-
 Pisum sativum CO2 + NADH + H+
-
 ?
1.17.1.9 S-formylglutathione + NAD+ lower KM-value than for formate but maximal activity is only 5.5% of that of formate Pisum sativum ?
-
 ?
1.17.1.9 S-formylthioglycolate + NAD+
-
 Pisum sativum ?
-
 ?
3.1.2.12 S-formylglutathione + H2O
-
 Pisum sativum glutathione + formate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.284 dimer 2 * 42100, SDS-PAGE Pisum sativum
1.17.1.9 dimer 2 * 42400, SDS-PAGE Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.284 6
-
 below, reaction with S-formylglutathione + NADH Pisum sativum
1.1.1.284 8 9 reaction with formaldehyde + NAD+ + glutathione Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.284 NAD+
-
 Pisum sativum
1.17.1.9 NAD+
-
 Pisum sativum