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Literature summary extracted from
- Sinapine esterase. 1. Characterization of sinapine esterase from cotyledons of Raphanus sativus (1979), Z. Naturforsch. C, 34, 715-720.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.49 | Acetylcholine | stimulation | Raphanus sativus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.49 | choline chloride | 100 mM 60% inhibition | Raphanus sativus | |
| 3.1.1.49 | diethyl p-nitrophenylphosphate | - |
Raphanus sativus | |
| 3.1.1.49 | diisopropyl fluorophosphate | - |
Raphanus sativus | |
| 3.1.1.49 | physostigmine | i.e. eserine | Raphanus sativus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.49 | 0.0195 | - |
Sinapoylcholine | - |
Raphanus sativus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.49 | Raphanus sativus | - |
red radish | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.49 | - |
Raphanus sativus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.49 | seed | - |
Raphanus sativus | - |
| 3.1.1.49 | seedling | - |
Raphanus sativus | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.1.49 | -20°C, tricine buffer, several weeks | Raphanus sativus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.49 | sinapoylcholine + H2O | - |
Raphanus sativus | sinapic acid + choline | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.49 | 35 | - |
- |
Raphanus sativus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.49 | 24 | 40 | less than 50% of maximal activity above and below | Raphanus sativus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.49 | 8.5 | - |
- |
Raphanus sativus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.49 | 5.5 | 10 | less than 50% of maximal activity above and below | Raphanus sativus |
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