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Literature summary extracted from

  • Stafforini, D.M.; Rollins, E.N.; Prescott, S.M.; McIntyre, T.M.
    The platelet-activating factor acetylhydrolase from human erythrocytes. Purification and properties (1993), J. Biol. Chem., 268, 3857-3865.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.1.47 highly sensitive to proteolysis Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.47 5,5'-dithiobis(2-nitrobenzoate)
-
 Homo sapiens
3.1.1.47 diethyldicarbonate
-
 Homo sapiens
3.1.1.47 dithioerythritol
-
 Homo sapiens
3.1.1.47 NaF
-
 Homo sapiens
3.1.1.47 p-bromophenacyl bromide
-
 Homo sapiens
3.1.1.47 p-nitroguanidinobenzoate
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.47 additional information no stimulation by Ca2+ or Mg2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.47 25000
-
 x * 25000 + x * 31000, the 25000 Da component represents the catalytic unit, SDS-PAGE Homo sapiens
3.1.1.47 31000
-
 x * 25000 + x * 31000, the 25000 Da component represents the catalytic unit, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.47 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O Homo sapiens i.e. PAF ?
-
 ?
3.1.1.47 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O Homo sapiens the substrate mediates a wide spectrum of actions, including hypotension, changes in smooth muscle tone, vascular permeability, signalling in inflammation and allergy. The intracelluar form of the enzyme regulates the accumulation of the substrate, e.g. in macrophages and platelets ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.47 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.47 from erythrocytes Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.47 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.47 0.805
-
 purified from erythrocytes Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.47 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O i.e. PAF Homo sapiens 1-O-alkyl-sn-glycero-3-phosphocholine + acetate
-
 ?
3.1.1.47 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O i.e. PAF Homo sapiens ?
-
 ?
3.1.1.47 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O the substrate mediates a wide spectrum of actions, including hypotension, changes in smooth muscle tone, vascular permeability, signalling in inflammation and allergy. The intracelluar form of the enzyme regulates the accumulation of the substrate, e.g. in macrophages and platelets Homo sapiens ?
-
 ?
3.1.1.47 1-palmitoyl-2-oxopentanoyl-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens 1-palmitoyl-sn-glycero-3-phosphocholine + 2-oxopentanoate
-
 ?
3.1.1.47 additional information the erythrocyte enzyme has a marked selectivity for short-chain acyl chains at sn-2 position, the linkage at the sn-1 postion may be of the ester or ether type and does not influence the catalytic effeciency Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.47 ? x * 25000 + x * 31000, the 25000 Da component represents the catalytic unit, SDS-PAGE Homo sapiens