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Literature summary extracted from

  • Berge, R.K.; Dossland, B.
    Differences between microsomal and mitochondrial-matrix palmitoyl-coenzyme A hydrolase, and palmitoyl-L-carnitine hydrolase from rat liver (1979), Biochem. J., 181, 119-125.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.2.2 3-hydroxybutyrate stimulates the mitochondrial-matrix enzyme activity Rattus norvegicus
3.1.2.2 ADP stimulates the microsomal activity Rattus norvegicus
3.1.2.2 ATP stimulates the microsomal activity Rattus norvegicus
3.1.2.2 citrate
-
 Rattus norvegicus
3.1.2.2 cyanide
-
 Rattus norvegicus
3.1.2.2 cysteine
-
 Rattus norvegicus
3.1.2.2 dithiothreitol stimulation of the microsomal enzyme Rattus norvegicus
3.1.2.2 EDTA stimulates the activity of the mitochondrial enzyme above 2 mM Rattus norvegicus
3.1.2.2 FAD stimulates the activity of the mitochondrial-matrix enzyme up to 0.4 mM Rattus norvegicus
3.1.2.2 FMN stimulates the microsomal activity Rattus norvegicus
3.1.2.2 fumarate stimulates the microsomal activity Rattus norvegicus
3.1.2.2 malate stimulates the mitochondrial-matrix enzyme activity Rattus norvegicus
3.1.2.2 NAD+ stimulates the microsomal activity Rattus norvegicus
3.1.2.2 nicotinic acid stimulates the microsomal activity Rattus norvegicus
3.1.2.2 riboflavin
-
 Rattus norvegicus
3.1.2.2 succinate stimulates the mitochondrial-matrix enzyme activity Rattus norvegicus
3.1.2.20 3-hydroxybutyrate stimulates the mitochondrial-matrix enzyme activity Rattus norvegicus
3.1.2.20 ADP stimulates the microsomal activity Rattus norvegicus
3.1.2.20 ATP stimulates the microsomal activity Rattus norvegicus
3.1.2.20 citrate
-
 Rattus norvegicus
3.1.2.20 cyanide
-
 Rattus norvegicus
3.1.2.20 cysteine
-
 Rattus norvegicus
3.1.2.20 dithiothreitol stimulation of the microsomal enzyme Rattus norvegicus
3.1.2.20 EDTA stimulates the activity of the mitochondrial enzyme above 2 mM Rattus norvegicus
3.1.2.20 FAD stimulates the activity of the mitochondrial-matrix enzyme up to 0.4 mM Rattus norvegicus
3.1.2.20 FMN stimulates the microsomal activity Rattus norvegicus
3.1.2.20 fumarate stimulates the microsomal activity Rattus norvegicus
3.1.2.20 malate stimulates the mitochondrial-matrix enzyme activity Rattus norvegicus
3.1.2.20 NAD+ stimulates the microsomal activity Rattus norvegicus
3.1.2.20 nicotinic acid stimulates the microsomal activity Rattus norvegicus
3.1.2.20 riboflavin
-
 Rattus norvegicus
3.1.2.20 succinate stimulates the mitochondrial-matrix enzyme activity Rattus norvegicus

General Stability

EC Number General Stability Organism
3.1.2.2 thawing and freezing inactivates Rattus norvegicus
3.1.2.20 thawing and freezing inactivates Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.28 Cu2+
-
 Rattus norvegicus
3.1.1.28 cysteine
-
 Rattus norvegicus
3.1.1.28 F-
-
 Rattus norvegicus
3.1.1.28 NO3-
-
 Rattus norvegicus
3.1.1.28 SO42-
-
 Rattus norvegicus
3.1.1.28 Zn2+
-
 Rattus norvegicus
3.1.2.2 ADP inhibits the mitochondrial-matrix enzyme Rattus norvegicus
3.1.2.2 ATP inhibits the mitochondrial-matrix enzyme Rattus norvegicus
3.1.2.2 beta-hydroxybutyrate moderately inhibits the microsomal enzyme activity Rattus norvegicus
3.1.2.2 Ca2+ inhibits the microsomal enzyme Rattus norvegicus
3.1.2.2 Co2+ slightly inhibits the mitochondrial enzyme Rattus norvegicus
3.1.2.2 Cu2+
-
 Rattus norvegicus
3.1.2.2 EDTA inhibits the activity of the mitochondrial enzyme at concentrations of 0.5 mM Rattus norvegicus
3.1.2.2 F-
-
 Rattus norvegicus
3.1.2.2 FAD slightly inhibits the microsomal enzyme Rattus norvegicus
3.1.2.2 FMN inhibits the mitochondrial-matrix enzyme activity Rattus norvegicus
3.1.2.2 fumarate inhibits the mitochondrial-matrix enzyme activity Rattus norvegicus
3.1.2.2 KCl inhibits the mitochondrial enzyme above 125 mM Rattus norvegicus
3.1.2.2 Mg2+ inhibits the microsomal enzyme, at higher concentrations also the mitochondrial enzyme Rattus norvegicus
3.1.2.2 Mn2+
-
 Rattus norvegicus
3.1.2.2 NAD+ inhibits the mitochondrial-matrix enzyme Rattus norvegicus
3.1.2.2 nicotinic acid inhibits the mitochondrial-matrix enzyme activity Rattus norvegicus
3.1.2.2 NO3-
-
 Rattus norvegicus
3.1.2.2 Pb2+ slightly inhibits the mitochondrial enzyme Rattus norvegicus
3.1.2.2 SO42-
-
 Rattus norvegicus
3.1.2.2 Triton X-100
-
 Rattus norvegicus
3.1.2.2 Zn2+
-
 Rattus norvegicus
3.1.2.20 ADP inhibits the mitochondrial-matrix enzyme Rattus norvegicus
3.1.2.20 ATP inhibits the mitochondrial-matrix enzyme Rattus norvegicus
3.1.2.20 beta-hydroxybutyrate moderately inhibits the microsomal enzyme activity Rattus norvegicus
3.1.2.20 Ca2+ inhibits the microsomal enzyme Rattus norvegicus
3.1.2.20 Co2+ slightly inhibits the mitochondrial enzyme Rattus norvegicus
3.1.2.20 Cu2+
-
 Rattus norvegicus
3.1.2.20 EDTA inhibits the activity of the mitochondrial enzyme at concentrations of 0.5 mM Rattus norvegicus
3.1.2.20 F-
-
 Rattus norvegicus
3.1.2.20 FAD slightly inhibits the microsomal enzyme Rattus norvegicus
3.1.2.20 FMN inhibits the mitochondrial-matrix enzyme activity Rattus norvegicus
3.1.2.20 fumarate inhibits the mitochondrial-matrix enzyme activity Rattus norvegicus
3.1.2.20 KCl inhibits the mitochondrial enzyme above 125 mM Rattus norvegicus
3.1.2.20 Mg2+ inhibits the microsomal enzyme, at higher concentrations also the mitochondrial enzyme Rattus norvegicus
3.1.2.20 Mn2+
-
 Rattus norvegicus
3.1.2.20 NAD+ inhibits the mitochondrial-matrix enzyme Rattus norvegicus
3.1.2.20 nicotinic acid inhibits the mitochondrial-matrix enzyme activity Rattus norvegicus
3.1.2.20 NO3-
-
 Rattus norvegicus
3.1.2.20 Pb2+ slightly inhibits the mitochondrial enzyme Rattus norvegicus
3.1.2.20 SO42-
-
 Rattus norvegicus
3.1.2.20 Triton X-100
-
 Rattus norvegicus
3.1.2.20 Zn2+
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.2 0.017
-
 hexadecanoyl-CoA
-
 Rattus norvegicus
3.1.2.2 0.047
-
 hexadecanoyl-CoA for the microsomal enzyme Rattus norvegicus
3.1.2.20 0.017
-
 hexadecanoyl-CoA
-
 Rattus norvegicus
3.1.2.20 0.047
-
 hexadecanoyl-CoA for the microsomal enzyme Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.28 microsome
-
 Rattus norvegicus
-
-
3.1.2.2 microsome
-
 Rattus norvegicus
-
-
3.1.2.2 mitochondrion
-
 Rattus norvegicus 5739
-
3.1.2.20 microsome
-
 Rattus norvegicus
-
-
3.1.2.20 mitochondrion
-
 Rattus norvegicus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.28 Ca2+ 10 mM moderately stimulates Rattus norvegicus
3.1.1.28 Mn2+ stimulates Rattus norvegicus
3.1.1.28 Pb2+ stimulates Rattus norvegicus
3.1.2.2 K+
-
 Rattus norvegicus
3.1.2.2 Mg2+ stimulates the mitochondrial enzyme by 0.5mM Rattus norvegicus
3.1.2.20 K+
-
 Rattus norvegicus
3.1.2.20 Mg2+ stimulates the mitochondrial enzyme by 0.5mM Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 palmitoyl-CoA + H2O Rattus norvegicus
-
 CoA + palmitate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O Rattus norvegicus
-
 CoA + palmitate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.28 Rattus norvegicus
-
-
-
3.1.2.2 Rattus norvegicus
-
-
-
3.1.2.20 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.28 liver
-
 Rattus norvegicus
-
3.1.2.2 liver
-
 Rattus norvegicus
-
3.1.2.20 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.2 0.063
-
-
 Rattus norvegicus
3.1.2.20 0.063
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 acetyl-CoA + H2O
-
 Rattus norvegicus CoA + acetate
-
 ?
3.1.2.2 acyl-CoA + H2O long-chain fatty-acyl-CoA Rattus norvegicus CoA + a carboxylate
-
 ?
3.1.2.2 cholesteryl oleate + H2O
-
 Rattus norvegicus cholesterol + oleate
-
 ?
3.1.2.2 dipalmitoylglycero-3-phosphocholine + H2O
-
 Rattus norvegicus ?
-
 ?
3.1.2.2 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Rattus norvegicus CoA + dodecanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O best substrate Rattus norvegicus CoA + hexadecanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Rattus norvegicus CoA + hexadecanoate
-
 ?
3.1.2.2 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Rattus norvegicus CoA + octadecanoate
-
 ?
3.1.2.2 oleoyl-CoA + H2O
-
 Rattus norvegicus CoA + oleate
-
 ?
3.1.2.2 palmitoyl-CoA + H2O
-
 Rattus norvegicus CoA + palmitate
-
 ?
3.1.2.2 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Rattus norvegicus CoA + tetradecanoate
-
 ?
3.1.2.2 tripalmitoylglycerol + H2O
-
 Rattus norvegicus ?
-
 ?
3.1.2.20 acetyl-CoA + H2O
-
 Rattus norvegicus CoA + acetate
-
 ?
3.1.2.20 acyl-CoA + H2O long-chain fatty-acyl-CoA Rattus norvegicus CoA + a carboxylate
-
 ?
3.1.2.20 cholesteryl oleate + H2O
-
 Rattus norvegicus cholesterol + oleate
-
 ?
3.1.2.20 dipalmitoylglycero-3-phosphocholine + H2O
-
 Rattus norvegicus ?
-
 ?
3.1.2.20 dodecanoyl-CoA + H2O i.e. lauroyl-CoA Rattus norvegicus CoA + dodecanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O best substrate Rattus norvegicus CoA + hexadecanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Rattus norvegicus CoA + hexadecanoate
-
 ?
3.1.2.20 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Rattus norvegicus CoA + octadecanoate
-
 ?
3.1.2.20 oleoyl-CoA + H2O
-
 Rattus norvegicus CoA + oleate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O
-
 Rattus norvegicus CoA + palmitate
-
 ?
3.1.2.20 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Rattus norvegicus CoA + tetradecanoate
-
 ?
3.1.2.20 tripalmitoylglycerol + H2O
-
 Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.28 42
-
-
 Rattus norvegicus
3.1.2.2 50
-
-
 Rattus norvegicus
3.1.2.20 50
-
-
 Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.28 35
-
 stable for 5 min Rattus norvegicus
3.1.1.28 45
-
 25% loss of activity after 15 min Rattus norvegicus
3.1.2.2 35
-
 stable Rattus norvegicus
3.1.2.2 45
-
 microsomal enzyme: stable, mitochondrial enzyme: unstable Rattus norvegicus
3.1.2.20 35
-
 stable Rattus norvegicus
3.1.2.20 45
-
 microsomal enzyme: stable, mitochondrial enzyme: unstable Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.28 7 8
-
 Rattus norvegicus
3.1.2.2 7 8.2
-
 Rattus norvegicus
3.1.2.20 7 8.2
-
 Rattus norvegicus