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Literature summary extracted from

  • Zhang, K.; Shiraki, M.; Saito, T.
    Purification of an extracellular D-(-)-3-hydroxybutyrate oligomer hydrolase from Pseudomonas sp. strain A1 and cloning and sequencing of its gene (1997), J. Bacteriol., 179, 72-77.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.22 expression in Escherichia coli Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.22 diisopropylfluorophosphate
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.22 0.17
-
 (R)-3-((R)-3-hydroxybutanoyloxy)2butanoate
-
 Pseudomonas sp.
3.1.1.22 0.18
-
 (R)-3-((R)-3-hydroxybutanoyloxy)butanoate
-
 Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.22 extracellular
-
 Pseudomonas sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.22 70000
-
 1 * 70000, SDS-PAGE Pseudomonas sp.
3.1.1.22 72880
-
 calculation from sequence of cDNA Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)3butanoate Pseudomonas sp. degradation of poly-(3-hydroxybutyrate) ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.22 Pseudomonas sp.
-
 strain a
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.22
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
3.1.1.22 -20°C, at least 6 months Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)11butanoate + H2O
-
 Pseudomonas sp. (R)-3-hydroxybutanoate
-
 ?
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O
-
 Pseudomonas sp. (R)-3-hydroxybutanoate
-
 ?
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)3butanoate degradation of poly-(3-hydroxybutyrate) Pseudomonas sp. ?
-
 ?
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)3butanoate + H2O
-
 Pseudomonas sp. (R)-3-hydroxybutanoate
-
 ?
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)4butanoate + H2O
-
 Pseudomonas sp. (R)-3-hydroxybutanoate
-
 ?
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)7butanoate + H2O
-
 Pseudomonas sp. (R)-3-hydroxybutanoate
-
 ?
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O i.e. D-(-)-3-[hydroxybutuyryloxy]butyric acid Pseudomonas sp. (R)-3-hydroxybutanoate
-
 ?
3.1.1.22 D-(-)3-(3-O-methyloxybutyryl)hydroxybutyrate + H2O
-
 Pseudomonas sp. ?
-
 ?
3.1.1.22 p-nitrophenyl acetate + H2O
-
 Pseudomonas sp. p-nitrophenol + acetate
-
 ?
3.1.1.22 p-nitrophenyl butyrate + H2O
-
 Pseudomonas sp. p-nitrophenol + butanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.22 monomer 1 * 70000, SDS-PAGE Pseudomonas sp.