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Literature summary extracted from

  • Shioi, Y.; Sasa, T.
    Purification of solubilized chlorophyllase from Chlorella protothecoides (1986), Methods Enzymol., 123, 421-427.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.14 synthesis useful tool for preparing chlorophyllides and chlorophyll derivatives esterified with various alcohols Auxenochlorella protothecoides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.14 0.002
-
chlorophyll a
-
Auxenochlorella protothecoides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.14 additional information Auxenochlorella protothecoides the enzyme participates in the phytyl ester formation in the final step of chlorophyll biosynthesis ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.14 Auxenochlorella protothecoides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.14
-
Auxenochlorella protothecoides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.14 0.96
-
-
Auxenochlorella protothecoides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.14 chlorophyll a + H2O
-
Auxenochlorella protothecoides chlorophyllide a + phytol
-
?
3.1.1.14 chlorophyll aXXX ester + alcohol
-
Auxenochlorella protothecoides chlorophyllide a(alcohol) + phytol
-
?
3.1.1.14 additional information the enzyme participates in the phytyl ester formation in the final step of chlorophyll biosynthesis Auxenochlorella protothecoides ?
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.14 6 8.5
-
Auxenochlorella protothecoides