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Literature summary extracted from

  • Yamane, M.; Takenoya, M.; Yajima, S.; Sue, M.
    Molecular and structural characterization of agmatine coumaroyltransferase in Triticeae, the key regulator of hydroxycinnamic acid amide accumulation (2021), Phytochemistry, 189, 112825.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.64 expressed in Escherichia coli BL21(DE3)pLysS cells Oryza sativa Japonica Group
2.3.1.64 expressed in Escherichia coli BL21CodonPlus(DE3)RP cells Hordeum murinum
2.3.1.64 expressed in Escherichia coli BL21CodonPlus(DE3)RP cells Hordeum vulgare
2.3.1.64 expressed in Escherichia coli BL21CodonPlus(DE3)RP cells Triticum aestivum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.64 isoform ACT2, hanging drop vapor diffusion method, using 100 mM MMT buffer pH 6.5, 25% (w/v) polyethylene glycol 1500 Triticum aestivum

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.64 E372A the mutant shows strongly reduced activity compared to the wild type enzyme Hordeum vulgare
2.3.1.64 E374A the mutant shows strongly reduced activity compared to the wild type enzyme Hordeum vulgare
2.3.1.64 W376A the mutant shows strongly reduced activity compared to the wild type enzyme Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.64 0.00036
-
 4-coumaroyl-CoA wild type enzyme, at pH 7.5 and 25°C Hordeum murinum
2.3.1.64 0.00056
-
 cinnamoyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 0.00057
-
 feruloyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 0.00061
-
 4-coumaroyl-CoA mutant enzyme E372A of isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 0.00074
-
 cinnamoyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 0.000789
-
 4-coumaroyl-CoA mutant enzyme W376A of isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 0.0008
-
 feruloyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 0.00088
-
 feruloyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 0.00092
-
 4-coumaroyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 0.00097
-
 4-coumaroyl-CoA wild type enzyme, at pH 7.5 and 25°C Oryza sativa Japonica Group
2.3.1.64 0.00106
-
 4-coumaroyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 0.00108
-
 4-coumaroyl-CoA mutant enzyme E374A of isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 0.00112
-
 cinnamoyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 0.00123
-
 4-coumaroyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 0.00126
-
 cinnamoyl-CoA wild type enzyme, at pH 7.5 and 25°C Hordeum murinum
2.3.1.64 0.00187
-
 caffeoyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 0.00191
-
 caffeoyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 0.00199
-
 caffeoyl-CoA wild type enzyme, at pH 7.5 and 25°C Oryza sativa Japonica Group
2.3.1.64 0.00201
-
 cinnamoyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 0.00221
-
 4-coumaroyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 0.00233
-
 cinnamoyl-CoA wild type enzyme, at pH 7.5 and 25°C Oryza sativa Japonica Group
2.3.1.64 0.00256
-
 feruloyl-CoA wild type enzyme, at pH 7.5 and 25°C Oryza sativa Japonica Group
2.3.1.64 0.0028
-
 caffeoyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 0.00285
-
 caffeoyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 0.00291
-
 caffeoyl-CoA wild type enzyme, at pH 7.5 and 25°C Hordeum murinum
2.3.1.64 0.00313
-
 agmatine wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 0.00387
-
 agmatine wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 0.0571
-
 agmatine mutant enzyme E374A of isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 0.131
-
 agmatine wild type enzyme isoform ACT2, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 0.146
-
 agmatine mutant enzyme E372A of isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 0.162
-
 agmatine wild type enzyme isoform ACT2, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 0.205
-
 agmatine wild type enzyme, at pH 7.5 and 25°C Hordeum murinum
2.3.1.64 125
-
 feruloyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 149
-
 feruloyl-CoA wild type enzyme, at pH 7.5 and 25°C Hordeum murinum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.64 4-coumaroyl-CoA + agmatine Hordeum murinum
-
 CoA + N-(4-guanidinobutyl)-4-hydroxycinnamamide
-
 ?
2.3.1.64 4-coumaroyl-CoA + agmatine Triticum aestivum
-
 CoA + N-(4-guanidinobutyl)-4-hydroxycinnamamide
-
 ?
2.3.1.64 4-coumaroyl-CoA + agmatine Hordeum vulgare
-
 CoA + N-(4-guanidinobutyl)-4-hydroxycinnamamide
-
 ?
2.3.1.64 4-coumaroyl-CoA + agmatine Oryza sativa Japonica Group
-
 CoA + N-(4-guanidinobutyl)-4-hydroxycinnamamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.64 Hordeum murinum
-
-
-
2.3.1.64 Hordeum vulgare A9ZPJ6 isoform ACT1-1; cultivars Betzes, Fibersnow, and Wasedori-nijo
-
2.3.1.64 Hordeum vulgare A9ZPJ7 isoform ACT2; cultivars Betzes, Fibersnow, and Wasedori-nijo
-
2.3.1.64 Oryza sativa Japonica Group Q7XPK7 cultivar Nipponbare
-
2.3.1.64 Triticum aestivum A0A3B6DKZ2 cultivar Chinese Spring
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.64 isoform ACT2 is purified by Talon metal affinity resin column chromatography, anion exchange chromatography, and gel filtration Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.64 shoot
-
 Hordeum murinum
-
2.3.1.64 shoot
-
 Triticum aestivum
-
2.3.1.64 shoot
-
 Hordeum vulgare
-
2.3.1.64 shoot
-
 Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.64 4-coumaroyl-CoA + agmatine
-
 Hordeum murinum CoA + N-(4-guanidinobutyl)-4-hydroxycinnamamide
-
 ?
2.3.1.64 4-coumaroyl-CoA + agmatine
-
 Triticum aestivum CoA + N-(4-guanidinobutyl)-4-hydroxycinnamamide
-
 ?
2.3.1.64 4-coumaroyl-CoA + agmatine
-
 Hordeum vulgare CoA + N-(4-guanidinobutyl)-4-hydroxycinnamamide
-
 ?
2.3.1.64 4-coumaroyl-CoA + agmatine
-
 Oryza sativa Japonica Group CoA + N-(4-guanidinobutyl)-4-hydroxycinnamamide
-
 ?
2.3.1.64 4-coumaroyl-CoA + putrescine
-
 Oryza sativa Japonica Group ?
-
 ?
2.3.1.64 4-coumaroyl-CoA + putrescine
-
 Hordeum murinum CoA + coumaroylputrescine
-
 ?
2.3.1.64 4-coumaroyl-CoA + putrescine
-
 Triticum aestivum CoA + coumaroylputrescine
-
 ?
2.3.1.64 4-coumaroyl-CoA + putrescine
-
 Hordeum vulgare CoA + coumaroylputrescine
-
 ?
2.3.1.64 4-coumaroyl-CoA + spermidine
-
 Oryza sativa Japonica Group ?
-
 ?
2.3.1.64 4-coumaroyl-CoA + spermidine
-
 Hordeum murinum CoA + coumaroylspermidine
-
 ?
2.3.1.64 4-coumaroyl-CoA + spermidine
-
 Triticum aestivum CoA + coumaroylspermidine
-
 ?
2.3.1.64 4-coumaroyl-CoA + spermidine
-
 Hordeum vulgare CoA + coumaroylspermidine
-
 ?
2.3.1.64 4-coumaroyl-CoA + spermine
-
 Oryza sativa Japonica Group ?
-
 ?
2.3.1.64 4-coumaroyl-CoA + spermine
-
 Hordeum murinum CoA + coumaroylspermine
-
 ?
2.3.1.64 4-coumaroyl-CoA + spermine
-
 Triticum aestivum CoA + coumaroylspermine
-
 ?
2.3.1.64 4-coumaroyl-CoA + spermine
-
 Hordeum vulgare CoA + coumaroylspermine
-
 ?
2.3.1.64 caffeoyl-CoA + agmatine
-
 Hordeum murinum CoA + caffeoylagmatine
-
 ?
2.3.1.64 caffeoyl-CoA + agmatine
-
 Triticum aestivum CoA + caffeoylagmatine
-
 ?
2.3.1.64 caffeoyl-CoA + agmatine
-
 Hordeum vulgare CoA + caffeoylagmatine
-
 ?
2.3.1.64 caffeoyl-CoA + agmatine
-
 Oryza sativa Japonica Group ?
-
 ?
2.3.1.64 cinnamoyl-CoA + agmatine
-
 Oryza sativa Japonica Group ?
-
 ?
2.3.1.64 cinnamoyl-CoA + agmatine
-
 Hordeum murinum CoA + cinnamoylagmatine
-
 ?
2.3.1.64 cinnamoyl-CoA + agmatine
-
 Triticum aestivum CoA + cinnamoylagmatine
-
 ?
2.3.1.64 cinnamoyl-CoA + agmatine
-
 Hordeum vulgare CoA + cinnamoylagmatine
-
 ?
2.3.1.64 feruloyl-CoA + agmatine
-
 Hordeum murinum CoA + feruloylagmatine
-
 ?
2.3.1.64 feruloyl-CoA + agmatine
-
 Triticum aestivum CoA + feruloylagmatine
-
 ?
2.3.1.64 feruloyl-CoA + agmatine
-
 Hordeum vulgare CoA + feruloylagmatine
-
 ?
2.3.1.64 feruloyl-CoA + agmatine
-
 Oryza sativa Japonica Group ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.64 ACT
-
 Hordeum murinum
2.3.1.64 ACT1-1 isoform Triticum aestivum
2.3.1.64 ACT1-1 isoform Hordeum vulgare
2.3.1.64 ACT2 isoform Triticum aestivum
2.3.1.64 ACT2 isoform Hordeum vulgare
2.3.1.64 agmatine p-coumaroyltransferase
-
 Hordeum murinum
2.3.1.64 agmatine p-coumaroyltransferase
-
 Triticum aestivum
2.3.1.64 agmatine p-coumaroyltransferase
-
 Hordeum vulgare
2.3.1.64 agmatine p-coumaroyltransferase
-
 Oryza sativa Japonica Group
2.3.1.64 AHT1
-
 Oryza sativa Japonica Group

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.64 0.008
-
 caffeoyl-CoA wild type enzyme, at pH 7.5 and 25°C Oryza sativa Japonica Group
2.3.1.64 0.01
-
 feruloyl-CoA wild type enzyme, at pH 7.5 and 25°C Oryza sativa Japonica Group
2.3.1.64 0.013
-
 4-coumaroyl-CoA wild type enzyme, at pH 7.5 and 25°C Oryza sativa Japonica Group
2.3.1.64 0.023
-
 cinnamoyl-CoA wild type enzyme, at pH 7.5 and 25°C Oryza sativa Japonica Group
2.3.1.64 0.068
-
 4-coumaroyl-CoA mutant enzyme W376A of isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 1.17
-
 agmatine mutant enzyme E372A of isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 1.33
-
 4-coumaroyl-CoA wild type enzyme, at pH 7.5 and 25°C Hordeum murinum
2.3.1.64 1.38
-
 4-coumaroyl-CoA mutant enzyme E372A of isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 3.22
-
 4-coumaroyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 3.92
-
 agmatine wild type enzyme isoform ACT2, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 4.73
-
 4-coumaroyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 6.28
-
 caffeoyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 6.4
-
 agmatine wild type enzyme, at pH 7.5 and 25°C Hordeum murinum
2.3.1.64 8.53
-
 feruloyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 8.79
-
 caffeoyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 9.3
-
 4-coumaroyl-CoA mutant enzyme E374A of isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 9.43
-
 4-coumaroyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 10
-
 caffeoyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 10.9
-
 agmatine mutant enzyme E374A of isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 12.2
-
 agmatine wild type enzyme isoform ACT2, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 13.1
-
 caffeoyl-CoA wild type enzyme, at pH 7.5 and 25°C Hordeum murinum
2.3.1.64 16.3
-
 cinnamoyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 16.7
-
 feruloyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 16.9
-
 cinnamoyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 18.1
-
 feruloyl-CoA wild type enzyme, at pH 7.5 and 25°C Hordeum murinum
2.3.1.64 19.8
-
 agmatine wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 20.1
-
 caffeoyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 21.9
-
 cinnamoyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 22.7
-
 feruloyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 24.1
-
 cinnamoyl-CoA wild type enzyme, at pH 7.5 and 25°C Hordeum murinum
2.3.1.64 34
-
 feruloyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 51
-
 4-coumaroyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 54.5
-
 agmatine wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 110
-
 cinnamoyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.64 4
-
 feruloyl-CoA wild type enzyme, at pH 7.5 and 25°C Oryza sativa Japonica Group
2.3.1.64 4
-
 caffeoyl-CoA wild type enzyme, at pH 7.5 and 25°C Oryza sativa Japonica Group
2.3.1.64 8
-
 agmatine mutant enzyme E372A of isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 10
-
 cinnamoyl-CoA wild type enzyme, at pH 7.5 and 25°C Oryza sativa Japonica Group
2.3.1.64 13
-
 4-coumaroyl-CoA wild type enzyme, at pH 7.5 and 25°C Oryza sativa Japonica Group
2.3.1.64 30
-
 agmatine wild type enzyme isoform ACT2, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 31
-
 agmatine wild type enzyme, at pH 7.5 and 25°C Hordeum murinum
2.3.1.64 75
-
 agmatine wild type enzyme isoform ACT2, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 86
-
 4-coumaroyl-CoA mutant enzyme W376A of isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 190
-
 agmatine mutant enzyme E374A of isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 1460
-
 4-coumaroyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 2270
-
 4-coumaroyl-CoA mutant enzyme E372A of isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 3140
-
 caffeoyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 3360
-
 caffeoyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 3510
-
 caffeoyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 3720
-
 4-coumaroyl-CoA wild type enzyme, at pH 7.5 and 25°C Hordeum murinum
2.3.1.64 4470
-
 4-coumaroyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 4510
-
 caffeoyl-CoA wild type enzyme, at pH 7.5 and 25°C Hordeum murinum
2.3.1.64 6330
-
 agmatine wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 7670
-
 4-coumaroyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 8590
-
 4-coumaroyl-CoA mutant enzyme E374A of isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 10500
-
 caffeoyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 10700
-
 feruloyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 12200
-
 feruloyl-CoA wild type enzyme, at pH 7.5 and 25°C Hordeum murinum
2.3.1.64 13400
-
 feruloyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 14100
-
 agmatine wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 15100
-
 cinnamoyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 19200
-
 cinnamoyl-CoA wild type enzyme, at pH 7.5 and 25°C Hordeum murinum
2.3.1.64 22000
-
 cinnamoyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 25900
-
 feruloyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Triticum aestivum
2.3.1.64 39000
-
 cinnamoyl-CoA wild type enzyme isoform ACT2, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 54600
-
 cinnamoyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 55400
-
 4-coumaroyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare
2.3.1.64 59600
-
 feruloyl-CoA wild type enzyme isoform ACT1-1, at pH 7.5 and 25°C Hordeum vulgare