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Literature summary extracted from

  • Wu, W.; Zhuang, Y.; Chen, D.; Ruan, Y.; Li, F.; Jackson, K.; Liu, C.W.; East, A.; Wen, J.; Tatsis, E.; Poole, P.S.; Xu, P.; Murray, J.D.
    Methylated chalcones are required for rhizobial nod gene induction in the Medicago truncatula rhizosphere (2024), New Phytol., 242, 2195-2206.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.154 gene MtOMT1, quantitative real-time PCR enzyme epression analysis, recombinant expression of His-tagged enzyme in Escherichia coli, recombinant expression in nodulated Medicago truncatula roots using Agrobacterium rhizogenes hairy root transformation Medicago truncatula
2.1.1.154 gene MtOMT3, quantitative real-time PCR enzyme epression analysis, recombinant expression of His-tagged enzyme in Escherichia coli, recombinant expression in nodulated Medicago truncatula roots using Agrobacterium rhizogenes hairy root transformation Medicago truncatula

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.154 additional information identification of the homozygous mutant lines NF10582 (chomt1-1, insertion at position 395), NF12032 (chomt1-2, insertion at position 95 bp), and NF16030 (chomt1-3, insertion at position 43 bp). Mutant roots have reduced nod gene-inducing flavonoids in their rhizospheres Medicago truncatula
2.1.1.154 additional information no mutants with insertions in ChOMT3 are found Medicago truncatula

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.154 0.0715
-
 isoliquiritigenin pH 7.5, 25°C, recombinant isozyme OMT1 Medicago truncatula
2.1.1.154 0.0809
-
 isoliquiritigenin pH 7.5, 25°C, recombinant isozyme OMT1 Medicago truncatula

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.154 S-adenosyl-L-methionine + isoliquiritigenin Medicago truncatula
-
 S-adenosyl-L-homocysteine + 2'-O-methylisoliquiritigenin
-
 ?
2.1.1.154 S-adenosyl-L-methionine + isoliquiritigenin Medicago truncatula R108
-
 S-adenosyl-L-homocysteine + 2'-O-methylisoliquiritigenin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.154 Medicago truncatula
-
-
-
2.1.1.154 Medicago truncatula R108
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.154 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Medicago truncatula

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.154 root hair ChOMT1 is specifically expressed in infected root hairs Medicago truncatula
-
2.1.1.154 root hair
-
 Medicago truncatula
-
2.1.1.154 seedling
-
 Medicago truncatula
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.154 additional information isozymes chalcone-OMT 1 (ChOMT1) and ChOMT3, but not OMT2, 4, and 5 in Medicago truncatula are able to produce 4,4'-dihydroxy-2'-methoxychalcone (DHMC) Medicago truncatula ?
-
-
2.1.1.154 additional information isozymes chalcone-OMT 1 (ChOMT1) and ChOMT3, but not OMT2, 4, and 5 in Medicago truncatula are able to produce 4,4'-dihydroxy-2'-methoxychalcone (DHMC) Medicago truncatula R108 ?
-
-
2.1.1.154 S-adenosyl-L-methionine + isoliquiritigenin
-
 Medicago truncatula S-adenosyl-L-homocysteine + 2'-O-methylisoliquiritigenin
-
 ?
2.1.1.154 S-adenosyl-L-methionine + isoliquiritigenin
-
 Medicago truncatula R108 S-adenosyl-L-homocysteine + 2'-O-methylisoliquiritigenin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.154 chalcone-O-methyltransferase
-
 Medicago truncatula
2.1.1.154 chalcone-O-methyltransferase 1
-
 Medicago truncatula
2.1.1.154 chalcone-O-methyltransferase 3
-
 Medicago truncatula
2.1.1.154 ChOMT1
-
 Medicago truncatula
2.1.1.154 ChOMT3
-
 Medicago truncatula
2.1.1.154 MtOMT1
-
 Medicago truncatula
2.1.1.154 MtOMT3
-
 Medicago truncatula

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.154 25
-
 assay at Medicago truncatula

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.154 0.12
-
 isoliquiritigenin pH 7.5, 25°C, recombinant isozyme OMT1 Medicago truncatula
2.1.1.154 0.15
-
 isoliquiritigenin pH 7.5, 25°C, recombinant isozyme OMT1 Medicago truncatula

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.154 7.5
-
 assay at Medicago truncatula

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.154 S-adenosyl-L-methionine
-
 Medicago truncatula

General Information

EC Number General Information Comment Organism
2.1.1.154 malfunction loss of ChOMT1 greatly reduces root DHMC levels Medicago truncatula
2.1.1.154 additional information methylation of isoliquiritigenin by MsChOMT involves the catalytic residues His278, Glu306 and Glu337. Three key substrate binding residues, Met189, Met329 and Thr332, which ensure the A-ring 2'-hydroxy is firmly positioned for deprotonation and methylation, are conserved Medicago truncatula
2.1.1.154 physiological function 2'-O-methylisoliquiritigenin strongly induces the nod gene in Medicago truncatula. ChOMT1 is important for nod gene activation in the rhizosphere, and ChOMT1 and 3 promote nodulation Medicago truncatula