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Literature summary extracted from

  • Park, H.L.; Yoo, Y.; Bhoo, S.H.; Lee, T.H.; Lee, S.W.; Cho, M.H.
    Two chalcone synthase isozymes participate redundantly in UV-induced sakuranetin synthesis in rice (2020), Int. J. Mol. Sci., 21, 3777.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.74 expressed in Escherichia coli BL21(DE3) cells Oryza sativa Japonica Group

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.74 0.02764
-
 4-coumaroyl-CoA isoform CHS8, at pH 7.2 and 30°C Oryza sativa Japonica Group
2.3.1.74 0.04544
-
 4-coumaroyl-CoA isoform CHS24, at pH 7.2 and 30°C Oryza sativa Japonica Group
2.3.1.74 0.04742
-
 malonyl-CoA isoform CHS24, at pH 7.2 and 30°C Oryza sativa Japonica Group
2.3.1.74 0.05938
-
 malonyl-CoA isoform CHS8, at pH 7.2 and 30°C Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.74 3 malonyl-CoA + 4-coumaroyl-CoA Oryza sativa Japonica Group
-
 4 CoA + naringenin chalcone + 3 CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.74 Oryza sativa Japonica Group
-
 cultivar Donjin
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.74 Ni-NTA agarose chromatography Oryza sativa Japonica Group

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.74 leaf sheath highest expression of isoform CHS24 Oryza sativa Japonica Group
-
2.3.1.74 panicle
-
 Oryza sativa Japonica Group
-
2.3.1.74 shoot
-
 Oryza sativa Japonica Group
-
2.3.1.74 stem
-
 Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.74 3 malonyl-CoA + 4-coumaroyl-CoA
-
 Oryza sativa Japonica Group 4 CoA + naringenin chalcone + 3 CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.74 ? x * 43900, isoform CHS8, calculated from amino acid sequence Oryza sativa Japonica Group
2.3.1.74 ? x * 43400, isoform CHS24, calculated from amino acid sequence Oryza sativa Japonica Group

Synonyms

EC Number Synonyms Comment Organism
2.3.1.74 CHS24 isoform Oryza sativa Japonica Group
2.3.1.74 Chs8 isoform Oryza sativa Japonica Group

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.74 0.000248
-
 4-coumaroyl-CoA isoform CHS8, at pH 7.2 and 30°C Oryza sativa Japonica Group
2.3.1.74 0.000862
-
 4-coumaroyl-CoA isoform CHS24, at pH 7.2 and 30°C Oryza sativa Japonica Group

Expression

EC Number Organism Comment Expression
2.3.1.74 Oryza sativa Japonica Group isoform CHS8 expression is markedly increased in the chs24 mutant under UV stress conditions compared to that in the wild type down

General Information

EC Number General Information Comment Organism
2.3.1.74 physiological function isoform CHS24 acts as the main chalcone isomerase isozyme and both isoforms CHS24 and CHS8 redundantly contribute to the UV-induced production of sakuranetin in rice leaves Oryza sativa Japonica Group

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.74 0.009
-
 4-coumaroyl-CoA isoform CHS8, at pH 7.2 and 30°C Oryza sativa Japonica Group
2.3.1.74 0.019
-
 4-coumaroyl-CoA isoform CHS24, at pH 7.2 and 30°C Oryza sativa Japonica Group