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Literature summary extracted from

  • Fang, J.; Wan, C.; Wang, W.; Ma, L.; Wang, X.; Cheng, C.; Zhou, J.; Qiao, Y.; Wang, X.
    Engineering herbicide-tolerance rice expressing an acetohydroxyacid synthase with a single amino acid deletion (2020), Int. J. Mol. Sci., 21, 1265.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.6 DELTAW548 deletion of residue W548 leads to the tetramer dissociation of the enzyme without reducing the activity. The deletion contributes to multi-family herbicides tolerance Oryza sativa Japonica Group

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 bispyribac-sodium
-
 Oryza sativa Japonica Group
2.2.1.6 chlorimuron-methyl
-
 Oryza sativa Japonica Group
2.2.1.6 flucarbazone-sodium
-
 Oryza sativa Japonica Group
2.2.1.6 imazethapyr
-
 Oryza sativa Japonica Group
2.2.1.6 rimsulfuron
-
 Oryza sativa Japonica Group
2.2.1.6 sulfometuron-methyl
-
 Oryza sativa Japonica Group

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.6 1 2.1 pyruvate mutant DELTAW548, presence of imazethapyr, pH 7.5, 37°C Oryza sativa Japonica Group
2.2.1.6 1 1.3 pyruvate mutant DELTAW548, presence of bispyribac-sodium, pH 7.5, 37°C Oryza sativa Japonica Group
2.2.1.6 9.2
-
 pyruvate wild-type, pH 7.5, 37°C Oryza sativa Japonica Group
2.2.1.6 9.2
-
 pyruvate mutant DELTAW548, presence of chlorimuron-methyl, pH 7.5, 37°C Oryza sativa Japonica Group
2.2.1.6 9.3
-
 pyruvate mutant DELTAW548, presence of sulfometuron-methyl, pH 7.5, 37°C Oryza sativa Japonica Group
2.2.1.6 9.4
-
 pyruvate mutant DELTAW548, pH 7.5, 37°C Oryza sativa Japonica Group
2.2.1.6 9.4
-
 pyruvate mutant DELTAW548, presence of rimsulfuron, pH 7.5, 37°C Oryza sativa Japonica Group
2.2.1.6 9.4
-
 pyruvate mutant DELTAW548, presence of flucarbazone-sodium, pH 7.5, 37°C Oryza sativa Japonica Group

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.6 128000
-
 main peak, plus smaller peak at 256000 Da for wild-type, the only peak for mutant DELTAW548, gel filtration Oryza sativa Japonica Group
2.2.1.6 256000
-
 minor peak, plus main peak at 124000 Da for wild-type, gel filtration Oryza sativa Japonica Group

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Oryza sativa Japonica Group Q6K2E8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 2 pyruvate
-
 Oryza sativa Japonica Group 2-acetolactate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.2.1.6 ALS1
-
 Oryza sativa Japonica Group

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2.1.6 5.1
-
 pyruvate mutant DELTAW548, presence of imazethapyr, pH 7.5, 37°C Oryza sativa Japonica Group
2.2.1.6 5.8
-
 pyruvate mutant DELTAW548, presence of bispyribac-sodium, pH 7.5, 37°C Oryza sativa Japonica Group
2.2.1.6 5.9
-
 pyruvate mutant DELTAW548, presence of flucarbazone-sodium, pH 7.5, 37°C Oryza sativa Japonica Group
2.2.1.6 6.5
-
 pyruvate mutant DELTAW548, presence of rimsulfuron, pH 7.5, 37°C Oryza sativa Japonica Group
2.2.1.6 6.5
-
 pyruvate mutant DELTAW548, presence of chlorimuron-methyl, pH 7.5, 37°C Oryza sativa Japonica Group
2.2.1.6 6.6
-
 pyruvate mutant DELTAW548, pH 7.5, 37°C Oryza sativa Japonica Group
2.2.1.6 6.6
-
 pyruvate mutant DELTAW548, presence of sulfometuron-methyl, pH 7.5, 37°C Oryza sativa Japonica Group
2.2.1.6 6.7
-
 pyruvate wild-type, pH 7.5, 37°C Oryza sativa Japonica Group

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.2.1.6 0.42
-
 pyruvate mutant DELTAW548, presence of imazethapyr, pH 7.5, 37°C Oryza sativa Japonica Group
2.2.1.6 0.51
-
 pyruvate mutant DELTAW548, presence of bispyribac-sodium, pH 7.5, 37°C Oryza sativa Japonica Group
2.2.1.6 0.63
-
 pyruvate mutant DELTAW548, presence of flucarbazone-sodium, pH 7.5, 37°C Oryza sativa Japonica Group
2.2.1.6 0.69
-
 pyruvate mutant DELTAW548, presence of rimsulfuron, pH 7.5, 37°C Oryza sativa Japonica Group
2.2.1.6 0.7
-
 pyruvate mutant DELTAW548, pH 7.5, 37°C Oryza sativa Japonica Group
2.2.1.6 0.71
-
 pyruvate wild-type, pH 7.5, 37°C Oryza sativa Japonica Group
2.2.1.6 0.71
-
 pyruvate mutant DELTAW548, presence of chlorimuron-methyl, pH 7.5, 37°C Oryza sativa Japonica Group
2.2.1.6 0.72
-
 pyruvate mutant DELTAW548, presence of sulfometuron-methyl, pH 7.5, 37°C Oryza sativa Japonica Group