Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yin, J.; Zhang, C.; Zhang, Q.; Long, F.; Hu, W.; Zhou, Y.; Mou, F.; Zhong, Y.; Wu, B.; Zhu, M.; Zou, L.; Zhu, X.
    A single amino acid substitution in the AAA-type ATPase LRD6-6 activates immune responses but decreases grain quality in rice (2024), Front. Plant Sci., 15, 1451897.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.4.6 generation of recombinant truncated His-tagged LRD6-6(125-487)P382S (covering the ATPase domain) protein Oryza sativa Japonica Group

Protein Variants

EC Number Protein Variants Comment Organism
3.6.4.6 additional information the spotted leaf mutant which we named spl-A (spotted leaf mutant from A814) is isolated from ethyl methanesulfonate (EMS) treated japonica rice A814 for its visible lesion mimic phenotype on the leaves when growing in the field. The mutation of the Spl-A locus has been previously reported as lesion mimic resembling (lmr), lesion resembling disease 6-6 (lrd6-6) and spotted leaf 4 (spl4) in rice Oryza sativa Japonica Group
3.6.4.6 P382S naturally occurring mutation, single nucleotide mutation of C1144T, cloning of the Spl-A gene by combining map-based cloning strategy and BSA sequencing. The mutation impairs LRD6-6 ATPase activity and disrupts its interaction with endosomal sorting complex required for transport (ESCRT)-III subunits OsSNF7.1/7.2/7.3, and thus, leading to compromise of multivesicular bodies (MVBs)-mediated vesicle trafficking and accumulation of ubiquitinated proteins in both leaves and seeds of the rice spl mutant named spl-A. Therefore, the immune response of mutant spl-A is activated, and the growth and grain quality are deteriorated Oryza sativa Japonica Group

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.4.6 Mg2+ required Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.4.6 ATP + H2O Oryza sativa Japonica Group
-
 ADP + phosphate
-
 ir
3.6.4.6 ATP + H2O Oryza sativa Japonica Group A814
-
 ADP + phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.6.4.6 Oryza sativa Japonica Group
-
-
-
3.6.4.6 Oryza sativa Japonica Group A814
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.4.6 leaf
-
 Oryza sativa Japonica Group
-
3.6.4.6 seedling
-
 Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.4.6 ATP + H2O
-
 Oryza sativa Japonica Group ADP + phosphate
-
 ir
3.6.4.6 ATP + H2O
-
 Oryza sativa Japonica Group A814 ADP + phosphate
-
 ir
3.6.4.6 additional information activity measurement by malachite green-based colorimetric method Oryza sativa Japonica Group ?
-
-
3.6.4.6 additional information activity measurement by malachite green-based colorimetric method Oryza sativa Japonica Group A814 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
3.6.4.6 AAA-type ATPase
-
 Oryza sativa Japonica Group
3.6.4.6 LRD6-6
-
 Oryza sativa Japonica Group

General Information

EC Number General Information Comment Organism
3.6.4.6 evolution the enzyme belongs to the AAA-type (ATPase associated with a variety of cell activities) ATPase superfamily Oryza sativa Japonica Group
3.6.4.6 malfunction rice spl mutant named spl-A (spotted leaf mutant from A814) shows autoimmunity, broad-spectrum disease resistance and growth deterioration including decreased rice quality. A single nucleotide mutation of C1144T leads to change of the 382nd proline to serine in the gene encoding the ATPases associated with diverse cellular activities (AAA)-type ATPase LRD6-6, the mutation is responsible for the phenotype of the spl-A mutant. The immune response of spl-A is activated, and the growth and grain quality are deteriorated Oryza sativa Japonica Group
3.6.4.6 additional information the conserved 382nd amino acid residue proline is essential for the full function of the AAA-type ATPase LRD6-6 Oryza sativa Japonica Group