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Literature summary extracted from

  • Read, B.J.; Cadzow, A.F.; Alphey, M.S.; Mitchell, J.B.O.; da Silva, R.G.
    Crystal structure, steady-state, and pre-steady-state kinetics of Acinetobacter baumannii ATP phosphoribosyltransferase (2024), Biochemistry, 63, 230-240.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.17 hanging drop vapor diffusion method, using 0.2 M sodium nitrate, 0.1 M bis-Tris propane pH 8.5, and 20% (w/v) polyethylene glycol 3350 Acinetobacter baumannii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.17 L-histidine histidine allosterically inhibits the enzyme in a negative feedback control mechanism Acinetobacter baumannii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.17 0.096
-
 5-phospho-alpha-D-ribose 1-diphosphate full length enzyme, with ADP as cosubstrate, in the presence of Mg2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 0.14
-
 5-phospho-alpha-D-ribose 1-diphosphate full length enzyme, with ATP as cosubstrate, in the presence of Mg2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 0.19
-
 ATP full length enzyme, in the presence of Mg2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 0.36
-
 ADP full length enzyme, in the presence of Mg2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 0.39
-
 ATP subunit HisGs, in the presence of Mn2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 0.44
-
 5-phospho-alpha-D-ribose 1-diphosphate subunit HisGs, with ADP as cosubstrate,in the presence of Mn2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 0.6
-
 5-phospho-alpha-D-ribose 1-diphosphate subunit HisGs, with ATP as cosubstrate, in the presence of Mg2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 0.6
-
 5-phospho-alpha-D-ribose 1-diphosphate subunit HisGs, with ATP as cosubstrate, in the presence of Mn2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 0.83
-
 ATP subunit HisGs, in the presence of Mg2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 1.2
-
 5-phospho-alpha-D-ribose 1-diphosphate subunit HisGs, with ADP as cosubstrate, in the presence of Mg2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 1.5
-
 ADP subunit HisGs, in the presence of Mg2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 2.2
-
 ADP subunit HisGs, in the presence of Mn2+, at pH 7.5 and 25°C Acinetobacter baumannii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.17 Mg2+ dependent on Acinetobacter baumannii
2.4.2.17 Mn2+ Mn2+ enhances subunit HisGS catalysis at 25°C Acinetobacter baumannii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate Acinetobacter baumannii
-
 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate Acinetobacter baumannii ACICU
-
 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.17 Acinetobacter baumannii A0A7U3XWM9 AND B2HWK9 subunits HisG and HisZ
-
2.4.2.17 Acinetobacter baumannii ACICU A0A7U3XWM9 AND B2HWK9 subunits HisG and HisZ
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.17 ADP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Acinetobacter baumannii 1-(5-phospho-beta-D-ribosyl)-ADP + diphosphate
-
 r
2.4.2.17 ADP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Acinetobacter baumannii ACICU 1-(5-phospho-beta-D-ribosyl)-ADP + diphosphate
-
 r
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Acinetobacter baumannii 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Acinetobacter baumannii ACICU 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.2.17 heterooctamer the catalytic subunit, HisGs, is allosterically activated upon binding of the regulatory subunit, HisZ, to form the heterooctameric holoenzyme Acinetobacter baumannii

Synonyms

EC Number Synonyms Comment Organism
2.4.2.17 ATPPRT
-
 Acinetobacter baumannii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.17 0.384
-
 ATP subunit HisGs, in the presence of Mg2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 0.48
-
 ADP subunit HisGs, in the presence of Mg2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 0.94
-
 ATP subunit HisGs, in the presence of Mn2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 3.3
-
 ADP subunit HisGs, in the presence of Mn2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 10.8
-
 ATP full length enzyme, in the presence of Mg2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 16.6
-
 ADP full length enzyme, in the presence of Mg2+, at pH 7.5 and 25°C Acinetobacter baumannii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.17 8 9.5
-
 Acinetobacter baumannii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.17 0.32
-
 ADP subunit HisGs, in the presence of Mg2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 0.4
-
 5-phospho-alpha-D-ribose 1-diphosphate subunit HisGs, with ADP as cosubstrate, in the presence of Mg2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 0.46
-
 ATP subunit HisGs, in the presence of Mg2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 0.64
-
 5-phospho-alpha-D-ribose 1-diphosphate subunit HisGs, with ATP as cosubstrate, in the presence of Mg2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 1.5
-
 ADP subunit HisGs, in the presence of Mn2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 1.6
-
 5-phospho-alpha-D-ribose 1-diphosphate subunit HisGs, with ATP as cosubstrate, in the presence of Mn2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 2.4
-
 ATP subunit HisGs, in the presence of Mn2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 8
-
 5-phospho-alpha-D-ribose 1-diphosphate subunit HisGs, with ADP as cosubstrate,in the presence of Mn2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 46
-
 ADP full length enzyme, in the presence of Mg2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 57
-
 ATP full length enzyme, in the presence of Mg2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 77
-
 5-phospho-alpha-D-ribose 1-diphosphate full length enzyme, with ATP as cosubstrate, in the presence of Mg2+, at pH 7.5 and 25°C Acinetobacter baumannii
2.4.2.17 170
-
 5-phospho-alpha-D-ribose 1-diphosphate full length enzyme, with ADP as cosubstrate, in the presence of Mg2+, at pH 7.5 and 25°C Acinetobacter baumannii