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Literature summary extracted from

  • Omar, I.; Crotti, M.; Li, C.; Pisak, K.; Czemerys, B.; Ferla, S.; van Noord, A.; Paul, C.E.; Karu, K.; Ozbalci, C.; Eggert, U.; Lloyd, R.; Barry, S.M.; Castagnolo, D.
    Insights into E. coli cyclopropane fatty acid synthase (CFAS) towards enantioselective carbene free biocatalytic cyclopropanation (2024), Angew. Chem. Int. Ed. Engl., 63, e202403493.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.16 expressed in Escherichia coli T7 cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.16 C345A the mutant shows little change from the wild type enzyme Escherichia coli
2.1.1.16 C354S the mutant shows little change from the wild type enzyme Escherichia coli
2.1.1.16 S270A the mutant enzyme is still able to cyclopropanate phosphatidylglycerol with comparable conversion to the wild type enzyme Escherichia coli
2.1.1.16 S270G the mutant enzyme is still able to cyclopropanate phosphatidylglycerol with comparable conversion to the wild type enzyme Escherichia coli
2.1.1.16 Y137F the mutation completely abolishes any catalytic activity on all phospholipids Escherichia coli
2.1.1.16 Y350F the mutant enzyme is still able to cyclopropanate phosphatidylglycerol with comparable conversion to the wild type enzyme, although the dicyclopropanated/monocyclopropanated ratio changed so that less dicyclopropanated is produced indicating a reduced affinity of the mutant for the monocyclopropanated lipid Escherichia coli
2.1.1.16 Y350F the mutation does not influence the binding mode of phosphatidylglycerol Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.16 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.16 3 S-adenosyl-L-methionine + 3 (2R)-3-[[(2,3-dihydroxypropoxy)(hydroxy)phosphoryl]oxy]-2-[[(9Z)-octadec-9-enoyl]oxy]propyl (9Z)-octadec-9-enoate
-
 Escherichia coli 3 S-adenosyl-L-homocysteine + (2R)-3-[[(2,3-dihydroxypropoxy)(hydroxy)phosphoryl]oxy]-2-[[8-(2-octylcyclopropyl)octanoyl]oxy]propyl (9Z)-octadec-9-enoate + (11R)-14,17,18-trihydroxy-1-(2-octylcyclopropyl)-8,14-dioxo-9,13,15-trioxa-14lambda5-phosphaoctadecan-11-yl (9Z)-octadec-9-enoate + (2R)-3-[[(2,3-dihydroxypropoxy)(hydroxy)phosphoryl]oxy]-2-[[8-(2-octylcyclopropyl)octanoyl]oxy]propyl 8-(2-octylcyclopropyl)octanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.16 homodimer
-
 Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.1.1.16 CFAS
-
 Escherichia coli
2.1.1.16 cyclopropane fatty acid synthase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.16 S-adenosyl-L-methionine
-
 Escherichia coli