Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Van Ende, M.; Timmermans, B.; Vanreppelen, G.; Siscar-Lewin, S.; Fischer, D.; Wijnants, S.; Romero, C.L.; Yazdani, S.; Rogiers, O.; Demuyser, L.; Van Zeebroeck, G.; Cen, Y.; Kuchler, K.; Brunke, S.; Van Dijck, P.
    The involvement of the Candida glabrata trehalase enzymes in stress resistance and gut colonization (2021), Virulence, 12, 329-345.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.28 gene ATH1, DNA and amino acid sequence determination and analysis, evolutionary relationship and phylogenetic tree Nakaseomyces glabratus
3.2.1.28 gene NTH1, DNA and amino acid sequence determination and analysis, evolutionary relationship and phylogenetic tree Nakaseomyces glabratus
3.2.1.28 gene NTH2, DNA and amino acid sequence determination and analysis, evolutionary relationship and phylogenetic tree Nakaseomyces glabratus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.28 additional information trehalase deletion strains are constructed in the ATCC2001 his3DELTA trp1DELTA leu2DELTA background, deletion of trehalase genes ath1, nth1, and nth2, construction of single and triple mutants, phenotypes, overview. The single Ath1 deletion strain shows a similar colonization as the wild type strain, while the triple deletion strain is not able to establish a stable colonization of the GI-tract, as over 66% of the mice clear the Candida glabrata administered at day 21. Deletion of ATH1 and/or NTH2 does not affect the growth in the presence of these stressors, while strains lacking NTH1 are more sensitive toward oxidative stress Nakaseomyces glabratus
3.2.1.28 additional information trehalase deletion strains are constructed in the ATCC2001 his3DELTA trp1DELTA leu2DELTA background, deletion of trehalase genes ath1, nth1, and nth2, construction of single and triple mutants, phenotypes, overview. Colonization in the GI tract is not maintained in the triple trehalase deletion strain. Deletion of ATH1 and/or NTH2 does not affect the growth in the presence of these stressors, while strains lacking NTH1 are more sensitive toward oxidative stress. This phenotype is observed in all strains lacking NTH1 with no additive effect of the other trehalases. All nth1DELTA strains show a decreased survival after acute exposure to different concentrations of hydrogen peroxide. The disruption of NTH1 does not affect growth in the presence of salt nor cell wall stress Nakaseomyces glabratus
3.2.1.28 additional information trehalase deletion strains are constructed in the ATCC2001 his3DELTA trp1DELTA leu2DELTA background, deletion of trehalase genes ath1, nth1, and nth2, construction of single and triple mutants, phenotypes, overview. Colonization in the GI tract is not maintained in the triple trehalase deletion strain. Deletion of ATH1 and/or NTH2 does not affect the growth in the presence of these stressors, while strains lacking NTH1 are more sensitive toward oxidative stress Nakaseomyces glabratus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.28 cell wall bound, orientated to the periplasmic space/ extracellular side Nakaseomyces glabratus 5618
-
3.2.1.28 cytoplasm
-
 Nakaseomyces glabratus 5737
-
3.2.1.28 extracellular
-
 Nakaseomyces glabratus
-
-
3.2.1.28 additional information isozyme Ath1 is predicted to contain one transmembrane (TM) domain at the N-terminus (between positions 83 and 103). The orthologous N-terminus and TM domain of ScAth1 confers the extracellular localization. Ath1 is not anchored to the cell membrane but is rather present in the periplasmic space or in the cell wall Nakaseomyces glabratus
-
-
3.2.1.28 additional information Nth1 amino acid sequence contains neither a transmembrane domain nor a signal sequence Nakaseomyces glabratus
-
-
3.2.1.28 additional information Nth2 amino acid sequence contains neither a transmembrane domain nor a signal sequence Nakaseomyces glabratus
-
-
3.2.1.28 periplasm
-
 Nakaseomyces glabratus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.28 Ca2+ required Nakaseomyces glabratus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.28 alpha,alpha-trehalose + H2O Nakaseomyces glabratus
-
 alpha-D-glucose + beta-D-glucose
-
 ?
3.2.1.28 alpha,alpha-trehalose + H2O Nakaseomyces glabratus CBS 138
-
 alpha-D-glucose + beta-D-glucose
-
 ?
3.2.1.28 alpha,alpha-trehalose + H2O Nakaseomyces glabratus ATCC 2001
-
 alpha-D-glucose + beta-D-glucose
-
 ?
3.2.1.28 alpha,alpha-trehalose + H2O Nakaseomyces glabratus BCRC 20586
-
 alpha-D-glucose + beta-D-glucose
-
 ?
3.2.1.28 alpha,alpha-trehalose + H2O Nakaseomyces glabratus JCM 3761
-
 alpha-D-glucose + beta-D-glucose
-
 ?
3.2.1.28 alpha,alpha-trehalose + H2O Nakaseomyces glabratus NBRC 0622
-
 alpha-D-glucose + beta-D-glucose
-
 ?
3.2.1.28 alpha,alpha-trehalose + H2O Nakaseomyces glabratus NRRL Y-65
-
 alpha-D-glucose + beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Nakaseomyces glabratus Q6FMU4
-
-
3.2.1.28 Nakaseomyces glabratus A0A0W0DQ98
-
-
3.2.1.28 Nakaseomyces glabratus Q6FWN0
-
-
3.2.1.28 Nakaseomyces glabratus ATCC 2001 Q6FMU4
-
-
3.2.1.28 Nakaseomyces glabratus ATCC 2001 A0A0W0DQ98
-
-
3.2.1.28 Nakaseomyces glabratus ATCC 2001 Q6FWN0
-
-
3.2.1.28 Nakaseomyces glabratus BCRC 20586 Q6FMU4
-
-
3.2.1.28 Nakaseomyces glabratus BCRC 20586 A0A0W0DQ98
-
-
3.2.1.28 Nakaseomyces glabratus BCRC 20586 Q6FWN0
-
-
3.2.1.28 Nakaseomyces glabratus CBS 138 Q6FMU4
-
-
3.2.1.28 Nakaseomyces glabratus CBS 138 A0A0W0DQ98
-
-
3.2.1.28 Nakaseomyces glabratus CBS 138 Q6FWN0
-
-
3.2.1.28 Nakaseomyces glabratus JCM 3761 Q6FMU4
-
-
3.2.1.28 Nakaseomyces glabratus JCM 3761 A0A0W0DQ98
-
-
3.2.1.28 Nakaseomyces glabratus JCM 3761 Q6FWN0
-
-
3.2.1.28 Nakaseomyces glabratus NBRC 0622 Q6FMU4
-
-
3.2.1.28 Nakaseomyces glabratus NBRC 0622 A0A0W0DQ98
-
-
3.2.1.28 Nakaseomyces glabratus NBRC 0622 Q6FWN0
-
-
3.2.1.28 Nakaseomyces glabratus NRRL Y-65 Q6FMU4
-
-
3.2.1.28 Nakaseomyces glabratus NRRL Y-65 A0A0W0DQ98
-
-
3.2.1.28 Nakaseomyces glabratus NRRL Y-65 Q6FWN0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 alpha,alpha-trehalose + H2O
-
 Nakaseomyces glabratus alpha-D-glucose + beta-D-glucose
-
 ?
3.2.1.28 alpha,alpha-trehalose + H2O
-
 Nakaseomyces glabratus CBS 138 alpha-D-glucose + beta-D-glucose
-
 ?
3.2.1.28 alpha,alpha-trehalose + H2O
-
 Nakaseomyces glabratus ATCC 2001 alpha-D-glucose + beta-D-glucose
-
 ?
3.2.1.28 alpha,alpha-trehalose + H2O
-
 Nakaseomyces glabratus BCRC 20586 alpha-D-glucose + beta-D-glucose
-
 ?
3.2.1.28 alpha,alpha-trehalose + H2O
-
 Nakaseomyces glabratus JCM 3761 alpha-D-glucose + beta-D-glucose
-
 ?
3.2.1.28 alpha,alpha-trehalose + H2O
-
 Nakaseomyces glabratus NBRC 0622 alpha-D-glucose + beta-D-glucose
-
 ?
3.2.1.28 alpha,alpha-trehalose + H2O
-
 Nakaseomyces glabratus NRRL Y-65 alpha-D-glucose + beta-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.28 ? x * 136500, about, sequence calculation Nakaseomyces glabratus
3.2.1.28 ? x * 87400, about, sequence calculation Nakaseomyces glabratus
3.2.1.28 ? x * 86500, about, sequence calculation Nakaseomyces glabratus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.28 Ath1
-
 Nakaseomyces glabratus
3.2.1.28 NTH1
-
 Nakaseomyces glabratus
3.2.1.28 NTH2
-
 Nakaseomyces glabratus
3.2.1.28 trehalase
-
 Nakaseomyces glabratus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.28 30
-
 assay at Nakaseomyces glabratus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.28 90
-
 purified recombinant enzyme, 5 min, inactivation Nakaseomyces glabratus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 4.5
-
 assay at Nakaseomyces glabratus

General Information

EC Number General Information Comment Organism
3.2.1.28 evolution isozymes Nth1 and Nth2 are highly similar with one another (71% amino acid identity), indicating that these enzymes are most probably the result of the whole genome duplication event Nakaseomyces glabratus
3.2.1.28 physiological function isozyme Ath1 is required for growth on extracellular trehalose as a carbon source. Ath1 is present at the extracellular side of the cells and hydrolyzes trehalose in the surrounding medium after which glucose is taken up by the glucose transporters. Trehalase plays only a minor role in the virulence of Candida glabrata in a mouse systemic infection model Nakaseomyces glabratus
3.2.1.28 physiological function trehalase plays only a minor role in the virulence of Candida glabrata in a mouse systemic infection model Nakaseomyces glabratus