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Literature summary extracted from

  • Cooper, C.; Bryant, M.; Hogan, N.; Johann, T.
    Investigations of amino acids in the 5-formyltetrahydrofolate binding site of 5,10-methenyltetrahydrofolate synthetase from Mycoplasma pneumonia (2019), Protein J., 38, 409-418.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.3.3.2 E55A no residual activity. Residue is important for binding 5-formyltetrahydrofolate in the active site and/or for substrate turnover of the enzyme Mycoplasmoides pneumoniae
6.3.3.2 E55Q no residual activity. Residue is important for binding 5-formyltetrahydrofolate in the active site and/or for substrate turnover of the enzyme Mycoplasmoides pneumoniae
6.3.3.2 F118A mutation results in substantial increases in apparent Km value for both 5-formyltetrahydrofolate and ATP, but does not substantially affect catalytic turnover. Residue is important for binding 5-formyltetrahydrofolate in the active site and/or for substrate turnover of the enzyme Mycoplasmoides pneumoniae
6.3.3.2 Q144A substantial increase in Km values Mycoplasmoides pneumoniae
6.3.3.2 Y122A increase in Km values Mycoplasmoides pneumoniae
6.3.3.2 Y122F increase in Km values Mycoplasmoides pneumoniae
6.3.3.2 Y123A no residual activity. Residue is important for binding 5-formyltetrahydrofolate in the active site and/or for substrate turnover of the enzyme Mycoplasmoides pneumoniae
6.3.3.2 Y123F increase in Km values Mycoplasmoides pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.3.2 0.076
-
 ATP wild-type, pH 6, 37°C Mycoplasmoides pneumoniae
6.3.3.2 0.38
-
 ATP mutant Y123F, pH 6, 37°C Mycoplasmoides pneumoniae
6.3.3.2 0.5 2 ATP mutant Y122F, pH 6, 37°C Mycoplasmoides pneumoniae
6.3.3.2 1 2 5-formyltetrahydrofolate mutant Q144A, pH 6, 37°C Mycoplasmoides pneumoniae
6.3.3.2 1.4
-
 ATP mutant Q144A, pH 6, 37°C Mycoplasmoides pneumoniae
6.3.3.2 2
-
 5-formyltetrahydrofolate mutant Y122F, pH 6, 37°C Mycoplasmoides pneumoniae
6.3.3.2 2.5
-
 5-formyltetrahydrofolate wild-type, pH 6, 37°C Mycoplasmoides pneumoniae
6.3.3.2 2.9
-
 ATP mutant F118A, pH 6, 37°C Mycoplasmoides pneumoniae
6.3.3.2 3.5
-
 ATP mutant Y122A, pH 6, 37°C Mycoplasmoides pneumoniae
6.3.3.2 3.7
-
 5-formyltetrahydrofolate mutant Y122A, pH 6, 37°C Mycoplasmoides pneumoniae
6.3.3.2 5.2
-
 5-formyltetrahydrofolate mutant Y123F, pH 6, 37°C Mycoplasmoides pneumoniae
6.3.3.2 20
-
 5-formyltetrahydrofolate mutant F118A, pH 6, 37°C Mycoplasmoides pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
6.3.3.2 Mycoplasmoides pneumoniae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.3.2 ATP + 5-formyltetrahydrofolate
-
 Mycoplasmoides pneumoniae ADP + phosphate + 5,10-methenyltetrahydrofolate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.3.2 0.74
-
 ATP mutant F118A, pH 6, 37°C Mycoplasmoides pneumoniae
6.3.3.2 0.97
-
 ATP mutant Y122A, pH 6, 37°C Mycoplasmoides pneumoniae
6.3.3.2 1
-
 ATP wild-type, pH 6, 37°C Mycoplasmoides pneumoniae
6.3.3.2 1.2
-
 ATP mutant Y123F, pH 6, 37°C Mycoplasmoides pneumoniae
6.3.3.2 1.7
-
 ATP mutant Y122F, pH 6, 37°C Mycoplasmoides pneumoniae
6.3.3.2 3.1
-
 ATP mutant Q144A, pH 6, 37°C Mycoplasmoides pneumoniae