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Literature summary extracted from

  • Baiya, S.; Pengthaisong, S.; Kitjaruwankul, S.; Ketudat, J.
    Structural analysis of rice Os4BGlu18 monolignol beta-glucosidase (2021), PLoS ONE, 16, e0241325.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.126 gene BGLU18, recombinant expression of N-terminally thioredoxin and His6-tagged Os4BGlu18 in Escherichia coli Oryza sativa Japonica Group

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.126 purified recombinant free Os4BGlu18 and in complex with delta-gluconolactone, hanging drop vapor diffusion method, mixing of 3 mg/ml protein in 100 mM MES, pH 6.0, with reservoir solution containing 21% PEG 3350, 100 mM MES, pH 6.0, at 15°C, method optimization, crystals of the Os4BGlu18 are soaked in 1 mM delta-gluconolactone in cryoprotectant for 20 min before vitrification to obtain the Os4BGlu18-DG complex, X-ray diffraction structure determination and analysis at 1.7 and 2.1 A resolution, respectively, structure modelling Oryza sativa Japonica Group

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.126 additional information Arabidopsis thaliana bglu45 mutant is rescued by expression of Os4BGlu18 displaying lower levels for all three substrates, coniferin, syringin, and 4-coumaryl alcohol glucoside, when compared with non-rescued line Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.126 4-coumaryl alcohol glucoside + H2O Oryza sativa Japonica Group
-
 D-glucose + 4-coumaryl alcohol
-
 ?
3.2.1.126 coniferin + H2O Oryza sativa Japonica Group
-
 D-glucose + coniferyl alcohol
-
 ?
3.2.1.126 additional information Oryza sativa Japonica Group rice Os4BGlu18 beta-glucosidase catalyzes hydrolysis of the monolignol glucosides, coniferin, syringin, and 4-coumaryl alcohol glucoside more efficiently than other natural substrates ?
-
-
3.2.1.126 syringin + H2O Oryza sativa Japonica Group
-
 D-glucose + sinapyl alcohol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.126 Oryza sativa Japonica Group Q7XSK0 EC 3.2.1.21
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.126 recombinant N-terminally thioredoxin and His6-tagged Os4BGlu18 from Escherichia coli by nickel affinity chromatography, and cleavage of the tag by enterokinase, followed by dialysis and ultrafiltration Oryza sativa Japonica Group

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.126 4-coumaryl alcohol glucoside + H2O
-
 Oryza sativa Japonica Group D-glucose + 4-coumaryl alcohol
-
 ?
3.2.1.126 coniferin + H2O
-
 Oryza sativa Japonica Group D-glucose + coniferyl alcohol
-
 ?
3.2.1.126 additional information rice Os4BGlu18 beta-glucosidase catalyzes hydrolysis of the monolignol glucosides, coniferin, syringin, and 4-coumaryl alcohol glucoside more efficiently than other natural substrates Oryza sativa Japonica Group ?
-
-
3.2.1.126 syringin + H2O
-
 Oryza sativa Japonica Group D-glucose + sinapyl alcohol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.126 beta-glucosidase 18
-
 Oryza sativa Japonica Group
3.2.1.126 BGLU18
-
 Oryza sativa Japonica Group
3.2.1.126 GH1 beta-glucosidase
-
 Oryza sativa Japonica Group
3.2.1.126 monolignol beta-glucosidase
-
 Oryza sativa Japonica Group
3.2.1.126 Os4BGlu18
-
 Oryza sativa Japonica Group

General Information

EC Number General Information Comment Organism
3.2.1.126 evolution the enzyme belongs to the glycoside hydrolase family 1, GH1 Oryza sativa Japonica Group
3.2.1.126 additional information the Os4BGlu18 structure exhibits the typical (beta/alpha)8 TIM barrel of glycoside hydrolase family 1 (GH1), but the four variable loops and two disulfide bonds appear significantly different from other known structures of GH1 beta-glucosidases. Molecular docking studies of the Os4BGlu18 structure with monolignol substrate ligands places the glycone in a similar position to the delta-gluconolactone in the complex structure and reveals the interactions between protein and ligands. Molecular docking, multiple sequence alignment, and homology modeling identify amino acid residues at the aglycone-binding site involved in substrate specificity for monolignol beta-glucosides. Molecular dynamics simulation. Structural basis of substrate recognition and hydrolysis by monolignol beta-glucosidases, overview Oryza sativa Japonica Group
3.2.1.126 physiological function rice Os4BGlu18 is a monolignol beta-glucosidase which exhibits high substrate specificity for the monolignol glucosides, coniferin, syringin, and p-coumaryl alcohol glucoside Oryza sativa Japonica Group

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.126 1.4
-
 4-coumaryl alcohol glucoside recombinant enzyme, pH and temperature not specified in the publication Oryza sativa Japonica Group
3.2.1.126 24
-
 syringin recombinant enzyme, pH and temperature not specified in the publication Oryza sativa Japonica Group
3.2.1.126 31.9
-
 coniferin recombinant enzyme, pH and temperature not specified in the publication Oryza sativa Japonica Group