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Literature summary extracted from

  • Park, S.I.; Park, H.L.; Bhoo, S.H.; Lee, S.W.; Cho, M.H.
    Biochemical and molecular characterization of the rice chalcone isomerase family (2021), Plants (Basel), 10, 2064.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.6 expressed in Escherichia coli BL21(DE3) and Rosetta 2 (DE3) cells Oryza sativa Japonica Group

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5.1.6 0.0116
-
 naringenin chalcone at pH 7.5, temperature not specified in the publication Oryza sativa Japonica Group
5.5.1.6 0.05095
-
 isoliquiritigenin at pH 7.5, temperature not specified in the publication Oryza sativa Japonica Group

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.6 Oryza sativa Japonica Group Q84T92 cultivar Dongjin
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.6 Ni-NTA agarose column chromatography Oryza sativa Japonica Group

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.5.1.6 leaf high expression Oryza sativa Japonica Group
-
5.5.1.6 panicle low expression Oryza sativa Japonica Group
-
5.5.1.6 root
-
 Oryza sativa Japonica Group
-
5.5.1.6 shoot
-
 Oryza sativa Japonica Group
-
5.5.1.6 stem
-
 Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.6 isoliquiritigenin
-
 Oryza sativa Japonica Group liquiritigenin
-
 ?
5.5.1.6 additional information isoforms CHI1, CHI6, and CHI7 have no activity Oryza sativa Japonica Group ?
-
-
5.5.1.6 naringenin chalcone isoform CHI3 exhibits a strong preference to naringenin chalcone compared to isoliquiritigenin Oryza sativa Japonica Group (2S)-naringenin
-
 ?

Subunits

EC Number Subunits Comment Organism
5.5.1.6 ? x * 28000, isoform CHI3, SDS-PAGE Oryza sativa Japonica Group

Synonyms

EC Number Synonyms Comment Organism
5.5.1.6 Chi3
-
 Oryza sativa Japonica Group

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5.1.6 0.00009214
-
 isoliquiritigenin at pH 7.5, temperature not specified in the publication Oryza sativa Japonica Group
5.5.1.6 69.35
-
 naringenin chalcone at pH 7.5, temperature not specified in the publication Oryza sativa Japonica Group

Expression

EC Number Organism Comment Expression
5.5.1.6 Oryza sativa Japonica Group isoform CHI3 expression is induced immediately in response to ultra-violet stress up

General Information

EC Number General Information Comment Organism
5.5.1.6 physiological function isoform CHI3 is involved in the biosynthesis of sakuranetin in rice Oryza sativa Japonica Group

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.5.1.6 0.001809
-
 isoliquiritigenin at pH 7.5, temperature not specified in the publication Oryza sativa Japonica Group
5.5.1.6 5978
-
 naringenin chalcone at pH 7.5, temperature not specified in the publication Oryza sativa Japonica Group